Job ID = 5720896 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-04-15T17:57:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T17:57:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 19,269,924 reads read : 19,269,924 reads written : 19,269,924 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:23 19269924 reads; of these: 19269924 (100.00%) were unpaired; of these: 519397 (2.70%) aligned 0 times 12848426 (66.68%) aligned exactly 1 time 5902101 (30.63%) aligned >1 times 97.30% overall alignment rate Time searching: 00:08:23 Overall time: 00:08:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8164591 / 18750527 = 0.4354 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 03:15:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 03:15:34: #1 read tag files... INFO @ Thu, 16 Apr 2020 03:15:34: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 03:15:41: 1000000 INFO @ Thu, 16 Apr 2020 03:15:47: 2000000 INFO @ Thu, 16 Apr 2020 03:15:54: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 03:16:00: 4000000 INFO @ Thu, 16 Apr 2020 03:16:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 03:16:04: #1 read tag files... INFO @ Thu, 16 Apr 2020 03:16:04: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 03:16:07: 5000000 INFO @ Thu, 16 Apr 2020 03:16:10: 1000000 INFO @ Thu, 16 Apr 2020 03:16:14: 6000000 INFO @ Thu, 16 Apr 2020 03:16:16: 2000000 INFO @ Thu, 16 Apr 2020 03:16:21: 7000000 INFO @ Thu, 16 Apr 2020 03:16:22: 3000000 INFO @ Thu, 16 Apr 2020 03:16:28: 4000000 INFO @ Thu, 16 Apr 2020 03:16:28: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 03:16:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 03:16:33: #1 read tag files... INFO @ Thu, 16 Apr 2020 03:16:33: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 03:16:34: 5000000 INFO @ Thu, 16 Apr 2020 03:16:35: 9000000 INFO @ Thu, 16 Apr 2020 03:16:39: 1000000 INFO @ Thu, 16 Apr 2020 03:16:40: 6000000 INFO @ Thu, 16 Apr 2020 03:16:41: 10000000 INFO @ Thu, 16 Apr 2020 03:16:45: 2000000 INFO @ Thu, 16 Apr 2020 03:16:45: #1 tag size is determined as 75 bps INFO @ Thu, 16 Apr 2020 03:16:45: #1 tag size = 75 INFO @ Thu, 16 Apr 2020 03:16:45: #1 total tags in treatment: 10585936 INFO @ Thu, 16 Apr 2020 03:16:45: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 03:16:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 03:16:45: #1 tags after filtering in treatment: 10585936 INFO @ Thu, 16 Apr 2020 03:16:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 03:16:45: #1 finished! INFO @ Thu, 16 Apr 2020 03:16:45: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 03:16:45: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 03:16:46: 7000000 INFO @ Thu, 16 Apr 2020 03:16:46: #2 number of paired peaks: 143 WARNING @ Thu, 16 Apr 2020 03:16:46: Fewer paired peaks (143) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 143 pairs to build model! INFO @ Thu, 16 Apr 2020 03:16:46: start model_add_line... INFO @ Thu, 16 Apr 2020 03:16:46: start X-correlation... INFO @ Thu, 16 Apr 2020 03:16:46: end of X-cor INFO @ Thu, 16 Apr 2020 03:16:46: #2 finished! INFO @ Thu, 16 Apr 2020 03:16:46: #2 predicted fragment length is 75 bps INFO @ Thu, 16 Apr 2020 03:16:46: #2 alternative fragment length(s) may be 75,564 bps INFO @ Thu, 16 Apr 2020 03:16:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.05_model.r WARNING @ Thu, 16 Apr 2020 03:16:46: #2 Since the d (75) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 03:16:46: #2 You may need to consider one of the other alternative d(s): 75,564 WARNING @ Thu, 16 Apr 2020 03:16:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 03:16:46: #3 Call peaks... INFO @ Thu, 16 Apr 2020 03:16:46: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 03:16:51: 3000000 INFO @ Thu, 16 Apr 2020 03:16:52: 8000000 INFO @ Thu, 16 Apr 2020 03:16:57: 4000000 INFO @ Thu, 16 Apr 2020 03:16:58: 9000000 INFO @ Thu, 16 Apr 2020 03:17:03: 5000000 INFO @ Thu, 16 Apr 2020 03:17:04: 10000000 INFO @ Thu, 16 Apr 2020 03:17:07: #1 tag size is determined as 75 bps INFO @ Thu, 16 Apr 2020 03:17:07: #1 tag size = 75 INFO @ Thu, 16 Apr 2020 03:17:07: #1 total tags in treatment: 10585936 INFO @ Thu, 16 Apr 2020 03:17:07: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 03:17:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 03:17:07: #1 tags after filtering in treatment: 10585936 INFO @ Thu, 16 Apr 2020 03:17:07: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 03:17:07: #1 finished! INFO @ Thu, 16 Apr 2020 03:17:07: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 03:17:07: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 03:17:07: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 03:17:08: #2 number of paired peaks: 143 WARNING @ Thu, 16 Apr 2020 03:17:08: Fewer paired peaks (143) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 143 pairs to build model! INFO @ Thu, 16 Apr 2020 03:17:08: start model_add_line... INFO @ Thu, 16 Apr 2020 03:17:08: start X-correlation... INFO @ Thu, 16 Apr 2020 03:17:08: end of X-cor INFO @ Thu, 16 Apr 2020 03:17:08: #2 finished! INFO @ Thu, 16 Apr 2020 03:17:08: #2 predicted fragment length is 75 bps INFO @ Thu, 16 Apr 2020 03:17:08: #2 alternative fragment length(s) may be 75,564 bps INFO @ Thu, 16 Apr 2020 03:17:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.10_model.r WARNING @ Thu, 16 Apr 2020 03:17:08: #2 Since the d (75) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 03:17:08: #2 You may need to consider one of the other alternative d(s): 75,564 WARNING @ Thu, 16 Apr 2020 03:17:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 03:17:08: #3 Call peaks... INFO @ Thu, 16 Apr 2020 03:17:08: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 03:17:09: 6000000 INFO @ Thu, 16 Apr 2020 03:17:14: 7000000 INFO @ Thu, 16 Apr 2020 03:17:17: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.05_peaks.xls INFO @ Thu, 16 Apr 2020 03:17:17: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 03:17:18: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.05_summits.bed INFO @ Thu, 16 Apr 2020 03:17:18: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (2867 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 03:17:20: 8000000 INFO @ Thu, 16 Apr 2020 03:17:25: 9000000 INFO @ Thu, 16 Apr 2020 03:17:30: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 03:17:31: 10000000 INFO @ Thu, 16 Apr 2020 03:17:34: #1 tag size is determined as 75 bps INFO @ Thu, 16 Apr 2020 03:17:34: #1 tag size = 75 INFO @ Thu, 16 Apr 2020 03:17:34: #1 total tags in treatment: 10585936 INFO @ Thu, 16 Apr 2020 03:17:34: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 03:17:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 03:17:34: #1 tags after filtering in treatment: 10585936 INFO @ Thu, 16 Apr 2020 03:17:34: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 03:17:34: #1 finished! INFO @ Thu, 16 Apr 2020 03:17:34: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 03:17:34: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 03:17:35: #2 number of paired peaks: 143 WARNING @ Thu, 16 Apr 2020 03:17:35: Fewer paired peaks (143) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 143 pairs to build model! INFO @ Thu, 16 Apr 2020 03:17:35: start model_add_line... INFO @ Thu, 16 Apr 2020 03:17:35: start X-correlation... INFO @ Thu, 16 Apr 2020 03:17:35: end of X-cor INFO @ Thu, 16 Apr 2020 03:17:35: #2 finished! INFO @ Thu, 16 Apr 2020 03:17:35: #2 predicted fragment length is 75 bps INFO @ Thu, 16 Apr 2020 03:17:35: #2 alternative fragment length(s) may be 75,564 bps INFO @ Thu, 16 Apr 2020 03:17:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.20_model.r WARNING @ Thu, 16 Apr 2020 03:17:35: #2 Since the d (75) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 03:17:35: #2 You may need to consider one of the other alternative d(s): 75,564 WARNING @ Thu, 16 Apr 2020 03:17:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 03:17:35: #3 Call peaks... INFO @ Thu, 16 Apr 2020 03:17:35: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 03:17:41: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.10_peaks.xls INFO @ Thu, 16 Apr 2020 03:17:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 03:17:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.10_summits.bed INFO @ Thu, 16 Apr 2020 03:17:41: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (1100 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 03:17:56: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 03:18:06: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.20_peaks.xls INFO @ Thu, 16 Apr 2020 03:18:06: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 03:18:06: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX5775924/SRX5775924.20_summits.bed INFO @ Thu, 16 Apr 2020 03:18:06: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (408 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。