Job ID = 6626521 SRX = SRX5736495 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 17147601 spots for SRR8956906/SRR8956906.sra Written 17147601 spots for SRR8956906/SRR8956906.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626735 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:35:38 17147601 reads; of these: 17147601 (100.00%) were paired; of these: 3977339 (23.19%) aligned concordantly 0 times 9754532 (56.89%) aligned concordantly exactly 1 time 3415730 (19.92%) aligned concordantly >1 times ---- 3977339 pairs aligned concordantly 0 times; of these: 1189765 (29.91%) aligned discordantly 1 time ---- 2787574 pairs aligned 0 times concordantly or discordantly; of these: 5575148 mates make up the pairs; of these: 3934079 (70.56%) aligned 0 times 701007 (12.57%) aligned exactly 1 time 940062 (16.86%) aligned >1 times 88.53% overall alignment rate Time searching: 00:35:38 Overall time: 00:35:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2896070 / 14348122 = 0.2018 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:04:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:04:15: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:04:15: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:04:22: 1000000 INFO @ Tue, 14 Jul 2020 08:04:28: 2000000 INFO @ Tue, 14 Jul 2020 08:04:35: 3000000 INFO @ Tue, 14 Jul 2020 08:04:41: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:04:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:04:45: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:04:45: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:04:48: 5000000 INFO @ Tue, 14 Jul 2020 08:04:52: 1000000 INFO @ Tue, 14 Jul 2020 08:04:55: 6000000 INFO @ Tue, 14 Jul 2020 08:04:59: 2000000 INFO @ Tue, 14 Jul 2020 08:05:01: 7000000 INFO @ Tue, 14 Jul 2020 08:05:06: 3000000 INFO @ Tue, 14 Jul 2020 08:05:08: 8000000 INFO @ Tue, 14 Jul 2020 08:05:13: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:05:15: 9000000 INFO @ Tue, 14 Jul 2020 08:05:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:05:15: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:05:15: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:05:20: 5000000 INFO @ Tue, 14 Jul 2020 08:05:22: 1000000 INFO @ Tue, 14 Jul 2020 08:05:22: 10000000 INFO @ Tue, 14 Jul 2020 08:05:26: 6000000 INFO @ Tue, 14 Jul 2020 08:05:28: 2000000 INFO @ Tue, 14 Jul 2020 08:05:29: 11000000 INFO @ Tue, 14 Jul 2020 08:05:33: 7000000 INFO @ Tue, 14 Jul 2020 08:05:35: 3000000 INFO @ Tue, 14 Jul 2020 08:05:37: 12000000 INFO @ Tue, 14 Jul 2020 08:05:40: 8000000 INFO @ Tue, 14 Jul 2020 08:05:42: 4000000 INFO @ Tue, 14 Jul 2020 08:05:44: 13000000 INFO @ Tue, 14 Jul 2020 08:05:46: 9000000 INFO @ Tue, 14 Jul 2020 08:05:48: 5000000 INFO @ Tue, 14 Jul 2020 08:05:51: 14000000 INFO @ Tue, 14 Jul 2020 08:05:52: 10000000 INFO @ Tue, 14 Jul 2020 08:05:54: 6000000 INFO @ Tue, 14 Jul 2020 08:05:58: 15000000 INFO @ Tue, 14 Jul 2020 08:05:59: 11000000 INFO @ Tue, 14 Jul 2020 08:06:01: 7000000 INFO @ Tue, 14 Jul 2020 08:06:05: 12000000 INFO @ Tue, 14 Jul 2020 08:06:05: 16000000 INFO @ Tue, 14 Jul 2020 08:06:07: 8000000 INFO @ Tue, 14 Jul 2020 08:06:11: 13000000 INFO @ Tue, 14 Jul 2020 08:06:12: 17000000 INFO @ Tue, 14 Jul 2020 08:06:14: 9000000 INFO @ Tue, 14 Jul 2020 08:06:18: 14000000 INFO @ Tue, 14 Jul 2020 08:06:19: 18000000 INFO @ Tue, 14 Jul 2020 08:06:20: 10000000 INFO @ Tue, 14 Jul 2020 08:06:24: 15000000 INFO @ Tue, 14 Jul 2020 08:06:26: 11000000 INFO @ Tue, 14 Jul 2020 08:06:26: 19000000 INFO @ Tue, 14 Jul 2020 08:06:30: 16000000 INFO @ Tue, 14 Jul 2020 08:06:33: 12000000 INFO @ Tue, 14 Jul 2020 08:06:33: 20000000 INFO @ Tue, 14 Jul 2020 08:06:37: 17000000 INFO @ Tue, 14 Jul 2020 08:06:39: 13000000 INFO @ Tue, 14 Jul 2020 08:06:40: 21000000 INFO @ Tue, 14 Jul 2020 08:06:43: 18000000 INFO @ Tue, 14 Jul 2020 08:06:46: 14000000 INFO @ Tue, 14 Jul 2020 08:06:47: 22000000 INFO @ Tue, 14 Jul 2020 08:06:49: 19000000 INFO @ Tue, 14 Jul 2020 08:06:52: 15000000 INFO @ Tue, 14 Jul 2020 08:06:54: 23000000 INFO @ Tue, 14 Jul 2020 08:06:56: 20000000 INFO @ Tue, 14 Jul 2020 08:06:58: 16000000 INFO @ Tue, 14 Jul 2020 08:07:01: 24000000 INFO @ Tue, 14 Jul 2020 08:07:03: 21000000 INFO @ Tue, 14 Jul 2020 08:07:05: 17000000 INFO @ Tue, 14 Jul 2020 08:07:05: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:07:05: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:07:05: #1 total tags in treatment: 10462451 INFO @ Tue, 14 Jul 2020 08:07:05: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:07:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:07:05: #1 tags after filtering in treatment: 9530267 INFO @ Tue, 14 Jul 2020 08:07:05: #1 Redundant rate of treatment: 0.09 INFO @ Tue, 14 Jul 2020 08:07:05: #1 finished! INFO @ Tue, 14 Jul 2020 08:07:05: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:07:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:07:06: #2 number of paired peaks: 79 WARNING @ Tue, 14 Jul 2020 08:07:06: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:07:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:07:09: 22000000 INFO @ Tue, 14 Jul 2020 08:07:11: 18000000 INFO @ Tue, 14 Jul 2020 08:07:15: 23000000 INFO @ Tue, 14 Jul 2020 08:07:17: 19000000 INFO @ Tue, 14 Jul 2020 08:07:21: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:07:24: 20000000 INFO @ Tue, 14 Jul 2020 08:07:25: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:07:25: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:07:25: #1 total tags in treatment: 10462451 INFO @ Tue, 14 Jul 2020 08:07:25: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:07:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:07:25: #1 tags after filtering in treatment: 9530267 INFO @ Tue, 14 Jul 2020 08:07:25: #1 Redundant rate of treatment: 0.09 INFO @ Tue, 14 Jul 2020 08:07:25: #1 finished! INFO @ Tue, 14 Jul 2020 08:07:25: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:07:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:07:26: #2 number of paired peaks: 79 WARNING @ Tue, 14 Jul 2020 08:07:26: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:07:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:07:30: 21000000 INFO @ Tue, 14 Jul 2020 08:07:36: 22000000 INFO @ Tue, 14 Jul 2020 08:07:41: 23000000 INFO @ Tue, 14 Jul 2020 08:07:47: 24000000 INFO @ Tue, 14 Jul 2020 08:07:50: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:07:50: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:07:50: #1 total tags in treatment: 10462451 INFO @ Tue, 14 Jul 2020 08:07:50: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:07:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:07:50: #1 tags after filtering in treatment: 9530267 INFO @ Tue, 14 Jul 2020 08:07:50: #1 Redundant rate of treatment: 0.09 INFO @ Tue, 14 Jul 2020 08:07:50: #1 finished! INFO @ Tue, 14 Jul 2020 08:07:50: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:07:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:07:51: #2 number of paired peaks: 79 WARNING @ Tue, 14 Jul 2020 08:07:51: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:07:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736495/SRX5736495.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。