Job ID = 6626502 SRX = SRX5736480 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 16267768 spots for SRR8956891/SRR8956891.sra Written 16267768 spots for SRR8956891/SRR8956891.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626722 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:37:35 16267768 reads; of these: 16267768 (100.00%) were paired; of these: 2091199 (12.85%) aligned concordantly 0 times 9785423 (60.15%) aligned concordantly exactly 1 time 4391146 (26.99%) aligned concordantly >1 times ---- 2091199 pairs aligned concordantly 0 times; of these: 450274 (21.53%) aligned discordantly 1 time ---- 1640925 pairs aligned 0 times concordantly or discordantly; of these: 3281850 mates make up the pairs; of these: 2439041 (74.32%) aligned 0 times 400934 (12.22%) aligned exactly 1 time 441875 (13.46%) aligned >1 times 92.50% overall alignment rate Time searching: 00:37:35 Overall time: 00:37:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1079319 / 14520148 = 0.0743 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:55:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:55:49: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:55:49: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:55:55: 1000000 INFO @ Tue, 14 Jul 2020 07:56:01: 2000000 INFO @ Tue, 14 Jul 2020 07:56:06: 3000000 INFO @ Tue, 14 Jul 2020 07:56:12: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:56:18: 5000000 INFO @ Tue, 14 Jul 2020 07:56:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:56:18: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:56:18: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:56:26: 6000000 INFO @ Tue, 14 Jul 2020 07:56:26: 1000000 INFO @ Tue, 14 Jul 2020 07:56:33: 7000000 INFO @ Tue, 14 Jul 2020 07:56:35: 2000000 INFO @ Tue, 14 Jul 2020 07:56:41: 8000000 INFO @ Tue, 14 Jul 2020 07:56:43: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:56:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:56:48: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:56:48: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:56:49: 9000000 INFO @ Tue, 14 Jul 2020 07:56:52: 4000000 INFO @ Tue, 14 Jul 2020 07:56:56: 1000000 INFO @ Tue, 14 Jul 2020 07:56:56: 10000000 INFO @ Tue, 14 Jul 2020 07:57:00: 5000000 INFO @ Tue, 14 Jul 2020 07:57:04: 11000000 INFO @ Tue, 14 Jul 2020 07:57:04: 2000000 INFO @ Tue, 14 Jul 2020 07:57:08: 6000000 INFO @ Tue, 14 Jul 2020 07:57:12: 12000000 INFO @ Tue, 14 Jul 2020 07:57:13: 3000000 INFO @ Tue, 14 Jul 2020 07:57:17: 7000000 INFO @ Tue, 14 Jul 2020 07:57:20: 13000000 INFO @ Tue, 14 Jul 2020 07:57:21: 4000000 INFO @ Tue, 14 Jul 2020 07:57:25: 8000000 INFO @ Tue, 14 Jul 2020 07:57:28: 14000000 INFO @ Tue, 14 Jul 2020 07:57:29: 5000000 INFO @ Tue, 14 Jul 2020 07:57:33: 9000000 INFO @ Tue, 14 Jul 2020 07:57:36: 15000000 INFO @ Tue, 14 Jul 2020 07:57:37: 6000000 INFO @ Tue, 14 Jul 2020 07:57:42: 10000000 INFO @ Tue, 14 Jul 2020 07:57:44: 16000000 INFO @ Tue, 14 Jul 2020 07:57:46: 7000000 INFO @ Tue, 14 Jul 2020 07:57:50: 11000000 INFO @ Tue, 14 Jul 2020 07:57:52: 17000000 INFO @ Tue, 14 Jul 2020 07:57:54: 8000000 INFO @ Tue, 14 Jul 2020 07:57:58: 12000000 INFO @ Tue, 14 Jul 2020 07:57:59: 18000000 INFO @ Tue, 14 Jul 2020 07:58:02: 9000000 INFO @ Tue, 14 Jul 2020 07:58:06: 13000000 INFO @ Tue, 14 Jul 2020 07:58:07: 19000000 INFO @ Tue, 14 Jul 2020 07:58:10: 10000000 INFO @ Tue, 14 Jul 2020 07:58:14: 14000000 INFO @ Tue, 14 Jul 2020 07:58:15: 20000000 INFO @ Tue, 14 Jul 2020 07:58:18: 11000000 INFO @ Tue, 14 Jul 2020 07:58:21: 15000000 INFO @ Tue, 14 Jul 2020 07:58:23: 21000000 INFO @ Tue, 14 Jul 2020 07:58:27: 12000000 INFO @ Tue, 14 Jul 2020 07:58:29: 16000000 INFO @ Tue, 14 Jul 2020 07:58:30: 22000000 INFO @ Tue, 14 Jul 2020 07:58:34: 13000000 INFO @ Tue, 14 Jul 2020 07:58:37: 17000000 INFO @ Tue, 14 Jul 2020 07:58:38: 23000000 INFO @ Tue, 14 Jul 2020 07:58:42: 14000000 INFO @ Tue, 14 Jul 2020 07:58:45: 18000000 INFO @ Tue, 14 Jul 2020 07:58:47: 24000000 INFO @ Tue, 14 Jul 2020 07:58:50: 15000000 INFO @ Tue, 14 Jul 2020 07:58:54: 19000000 INFO @ Tue, 14 Jul 2020 07:58:54: 25000000 INFO @ Tue, 14 Jul 2020 07:58:58: 16000000 INFO @ Tue, 14 Jul 2020 07:59:02: 20000000 INFO @ Tue, 14 Jul 2020 07:59:02: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:59:06: 17000000 INFO @ Tue, 14 Jul 2020 07:59:10: 27000000 INFO @ Tue, 14 Jul 2020 07:59:10: 21000000 INFO @ Tue, 14 Jul 2020 07:59:14: 18000000 INFO @ Tue, 14 Jul 2020 07:59:17: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 07:59:17: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 07:59:17: #1 total tags in treatment: 13115797 INFO @ Tue, 14 Jul 2020 07:59:17: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:59:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:59:17: #1 tags after filtering in treatment: 11669531 INFO @ Tue, 14 Jul 2020 07:59:17: #1 Redundant rate of treatment: 0.11 INFO @ Tue, 14 Jul 2020 07:59:17: #1 finished! INFO @ Tue, 14 Jul 2020 07:59:17: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:59:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:59:18: #2 number of paired peaks: 26 WARNING @ Tue, 14 Jul 2020 07:59:18: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:59:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:59:18: 22000000 INFO @ Tue, 14 Jul 2020 07:59:22: 19000000 INFO @ Tue, 14 Jul 2020 07:59:27: 23000000 INFO @ Tue, 14 Jul 2020 07:59:31: 20000000 INFO @ Tue, 14 Jul 2020 07:59:34: 24000000 INFO @ Tue, 14 Jul 2020 07:59:39: 21000000 INFO @ Tue, 14 Jul 2020 07:59:42: 25000000 INFO @ Tue, 14 Jul 2020 07:59:48: 22000000 INFO @ Tue, 14 Jul 2020 07:59:51: 26000000 INFO @ Tue, 14 Jul 2020 07:59:57: 23000000 INFO @ Tue, 14 Jul 2020 07:59:59: 27000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:00:04: 24000000 INFO @ Tue, 14 Jul 2020 08:00:07: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:00:07: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:00:07: #1 total tags in treatment: 13115797 INFO @ Tue, 14 Jul 2020 08:00:07: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:00:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:00:07: #1 tags after filtering in treatment: 11669531 INFO @ Tue, 14 Jul 2020 08:00:07: #1 Redundant rate of treatment: 0.11 INFO @ Tue, 14 Jul 2020 08:00:07: #1 finished! INFO @ Tue, 14 Jul 2020 08:00:07: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:00:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:00:08: #2 number of paired peaks: 26 WARNING @ Tue, 14 Jul 2020 08:00:08: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:00:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:00:12: 25000000 INFO @ Tue, 14 Jul 2020 08:00:20: 26000000 INFO @ Tue, 14 Jul 2020 08:00:27: 27000000 INFO @ Tue, 14 Jul 2020 08:00:35: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:00:35: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:00:35: #1 total tags in treatment: 13115797 INFO @ Tue, 14 Jul 2020 08:00:35: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:00:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:00:35: #1 tags after filtering in treatment: 11669531 INFO @ Tue, 14 Jul 2020 08:00:35: #1 Redundant rate of treatment: 0.11 INFO @ Tue, 14 Jul 2020 08:00:35: #1 finished! INFO @ Tue, 14 Jul 2020 08:00:35: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:00:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:00:35: #2 number of paired peaks: 26 WARNING @ Tue, 14 Jul 2020 08:00:35: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:00:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736480/SRX5736480.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling