Job ID = 6626478 SRX = SRX5736461 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 32708081 spots for SRR8956873/SRR8956873.sra Written 32708081 spots for SRR8956873/SRR8956873.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626964 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:19:50 32708081 reads; of these: 32708081 (100.00%) were paired; of these: 4424960 (13.53%) aligned concordantly 0 times 18989218 (58.06%) aligned concordantly exactly 1 time 9293903 (28.41%) aligned concordantly >1 times ---- 4424960 pairs aligned concordantly 0 times; of these: 122127 (2.76%) aligned discordantly 1 time ---- 4302833 pairs aligned 0 times concordantly or discordantly; of these: 8605666 mates make up the pairs; of these: 7377846 (85.73%) aligned 0 times 619474 (7.20%) aligned exactly 1 time 608346 (7.07%) aligned >1 times 88.72% overall alignment rate Time searching: 01:19:50 Overall time: 01:19:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4411942 / 28362451 = 0.1556 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:42:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:42:29: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:42:29: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:42:36: 1000000 INFO @ Tue, 14 Jul 2020 08:42:42: 2000000 INFO @ Tue, 14 Jul 2020 08:42:49: 3000000 INFO @ Tue, 14 Jul 2020 08:42:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:42:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:42:59: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:42:59: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:43:01: 5000000 INFO @ Tue, 14 Jul 2020 08:43:06: 1000000 INFO @ Tue, 14 Jul 2020 08:43:08: 6000000 INFO @ Tue, 14 Jul 2020 08:43:12: 2000000 INFO @ Tue, 14 Jul 2020 08:43:14: 7000000 INFO @ Tue, 14 Jul 2020 08:43:19: 3000000 INFO @ Tue, 14 Jul 2020 08:43:20: 8000000 INFO @ Tue, 14 Jul 2020 08:43:25: 4000000 INFO @ Tue, 14 Jul 2020 08:43:27: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:43:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:43:29: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:43:29: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:43:31: 5000000 INFO @ Tue, 14 Jul 2020 08:43:33: 10000000 INFO @ Tue, 14 Jul 2020 08:43:36: 1000000 INFO @ Tue, 14 Jul 2020 08:43:38: 6000000 INFO @ Tue, 14 Jul 2020 08:43:40: 11000000 INFO @ Tue, 14 Jul 2020 08:43:42: 2000000 INFO @ Tue, 14 Jul 2020 08:43:44: 7000000 INFO @ Tue, 14 Jul 2020 08:43:46: 12000000 INFO @ Tue, 14 Jul 2020 08:43:49: 3000000 INFO @ Tue, 14 Jul 2020 08:43:51: 8000000 INFO @ Tue, 14 Jul 2020 08:43:53: 13000000 INFO @ Tue, 14 Jul 2020 08:43:56: 4000000 INFO @ Tue, 14 Jul 2020 08:43:58: 9000000 INFO @ Tue, 14 Jul 2020 08:44:01: 14000000 INFO @ Tue, 14 Jul 2020 08:44:04: 5000000 INFO @ Tue, 14 Jul 2020 08:44:05: 10000000 INFO @ Tue, 14 Jul 2020 08:44:08: 15000000 INFO @ Tue, 14 Jul 2020 08:44:11: 6000000 INFO @ Tue, 14 Jul 2020 08:44:12: 11000000 INFO @ Tue, 14 Jul 2020 08:44:16: 16000000 INFO @ Tue, 14 Jul 2020 08:44:18: 7000000 INFO @ Tue, 14 Jul 2020 08:44:19: 12000000 INFO @ Tue, 14 Jul 2020 08:44:23: 17000000 INFO @ Tue, 14 Jul 2020 08:44:25: 8000000 INFO @ Tue, 14 Jul 2020 08:44:26: 13000000 INFO @ Tue, 14 Jul 2020 08:44:30: 18000000 INFO @ Tue, 14 Jul 2020 08:44:32: 9000000 INFO @ Tue, 14 Jul 2020 08:44:32: 14000000 INFO @ Tue, 14 Jul 2020 08:44:37: 19000000 INFO @ Tue, 14 Jul 2020 08:44:39: 15000000 INFO @ Tue, 14 Jul 2020 08:44:39: 10000000 INFO @ Tue, 14 Jul 2020 08:44:44: 20000000 INFO @ Tue, 14 Jul 2020 08:44:45: 16000000 INFO @ Tue, 14 Jul 2020 08:44:46: 11000000 INFO @ Tue, 14 Jul 2020 08:44:50: 21000000 INFO @ Tue, 14 Jul 2020 08:44:52: 17000000 INFO @ Tue, 14 Jul 2020 08:44:52: 12000000 INFO @ Tue, 14 Jul 2020 08:44:57: 22000000 INFO @ Tue, 14 Jul 2020 08:44:59: 18000000 INFO @ Tue, 14 Jul 2020 08:44:59: 13000000 INFO @ Tue, 14 Jul 2020 08:45:04: 23000000 INFO @ Tue, 14 Jul 2020 08:45:05: 19000000 INFO @ Tue, 14 Jul 2020 08:45:06: 14000000 INFO @ Tue, 14 Jul 2020 08:45:10: 24000000 INFO @ Tue, 14 Jul 2020 08:45:12: 20000000 INFO @ Tue, 14 Jul 2020 08:45:12: 15000000 INFO @ Tue, 14 Jul 2020 08:45:17: 25000000 INFO @ Tue, 14 Jul 2020 08:45:18: 21000000 INFO @ Tue, 14 Jul 2020 08:45:19: 16000000 INFO @ Tue, 14 Jul 2020 08:45:23: 26000000 INFO @ Tue, 14 Jul 2020 08:45:25: 22000000 INFO @ Tue, 14 Jul 2020 08:45:25: 17000000 INFO @ Tue, 14 Jul 2020 08:45:30: 27000000 INFO @ Tue, 14 Jul 2020 08:45:31: 23000000 INFO @ Tue, 14 Jul 2020 08:45:32: 18000000 INFO @ Tue, 14 Jul 2020 08:45:36: 28000000 INFO @ Tue, 14 Jul 2020 08:45:37: 24000000 INFO @ Tue, 14 Jul 2020 08:45:38: 19000000 INFO @ Tue, 14 Jul 2020 08:45:42: 29000000 INFO @ Tue, 14 Jul 2020 08:45:44: 25000000 INFO @ Tue, 14 Jul 2020 08:45:45: 20000000 INFO @ Tue, 14 Jul 2020 08:45:49: 30000000 INFO @ Tue, 14 Jul 2020 08:45:50: 26000000 INFO @ Tue, 14 Jul 2020 08:45:51: 21000000 INFO @ Tue, 14 Jul 2020 08:45:55: 31000000 INFO @ Tue, 14 Jul 2020 08:45:56: 27000000 INFO @ Tue, 14 Jul 2020 08:45:58: 22000000 INFO @ Tue, 14 Jul 2020 08:46:02: 32000000 INFO @ Tue, 14 Jul 2020 08:46:03: 28000000 INFO @ Tue, 14 Jul 2020 08:46:04: 23000000 INFO @ Tue, 14 Jul 2020 08:46:08: 33000000 INFO @ Tue, 14 Jul 2020 08:46:09: 29000000 INFO @ Tue, 14 Jul 2020 08:46:10: 24000000 INFO @ Tue, 14 Jul 2020 08:46:14: 34000000 INFO @ Tue, 14 Jul 2020 08:46:15: 30000000 INFO @ Tue, 14 Jul 2020 08:46:17: 25000000 INFO @ Tue, 14 Jul 2020 08:46:21: 35000000 INFO @ Tue, 14 Jul 2020 08:46:22: 31000000 INFO @ Tue, 14 Jul 2020 08:46:23: 26000000 INFO @ Tue, 14 Jul 2020 08:46:27: 36000000 INFO @ Tue, 14 Jul 2020 08:46:28: 32000000 INFO @ Tue, 14 Jul 2020 08:46:29: 27000000 INFO @ Tue, 14 Jul 2020 08:46:33: 37000000 INFO @ Tue, 14 Jul 2020 08:46:34: 33000000 INFO @ Tue, 14 Jul 2020 08:46:35: 28000000 INFO @ Tue, 14 Jul 2020 08:46:40: 38000000 INFO @ Tue, 14 Jul 2020 08:46:40: 34000000 INFO @ Tue, 14 Jul 2020 08:46:42: 29000000 INFO @ Tue, 14 Jul 2020 08:46:46: 39000000 INFO @ Tue, 14 Jul 2020 08:46:46: 35000000 INFO @ Tue, 14 Jul 2020 08:46:48: 30000000 INFO @ Tue, 14 Jul 2020 08:46:52: 40000000 INFO @ Tue, 14 Jul 2020 08:46:53: 36000000 INFO @ Tue, 14 Jul 2020 08:46:54: 31000000 INFO @ Tue, 14 Jul 2020 08:46:58: 41000000 INFO @ Tue, 14 Jul 2020 08:46:59: 37000000 INFO @ Tue, 14 Jul 2020 08:47:01: 32000000 INFO @ Tue, 14 Jul 2020 08:47:05: 42000000 INFO @ Tue, 14 Jul 2020 08:47:05: 38000000 INFO @ Tue, 14 Jul 2020 08:47:07: 33000000 INFO @ Tue, 14 Jul 2020 08:47:11: 43000000 INFO @ Tue, 14 Jul 2020 08:47:12: 39000000 INFO @ Tue, 14 Jul 2020 08:47:13: 34000000 INFO @ Tue, 14 Jul 2020 08:47:17: 44000000 INFO @ Tue, 14 Jul 2020 08:47:18: 40000000 INFO @ Tue, 14 Jul 2020 08:47:19: 35000000 INFO @ Tue, 14 Jul 2020 08:47:23: 45000000 INFO @ Tue, 14 Jul 2020 08:47:24: 41000000 INFO @ Tue, 14 Jul 2020 08:47:26: 36000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:47:30: 46000000 INFO @ Tue, 14 Jul 2020 08:47:30: 42000000 INFO @ Tue, 14 Jul 2020 08:47:32: 37000000 INFO @ Tue, 14 Jul 2020 08:47:36: 47000000 INFO @ Tue, 14 Jul 2020 08:47:36: 43000000 INFO @ Tue, 14 Jul 2020 08:47:38: 38000000 INFO @ Tue, 14 Jul 2020 08:47:42: 48000000 INFO @ Tue, 14 Jul 2020 08:47:43: 44000000 INFO @ Tue, 14 Jul 2020 08:47:45: 39000000 INFO @ Tue, 14 Jul 2020 08:47:48: 49000000 INFO @ Tue, 14 Jul 2020 08:47:49: 45000000 INFO @ Tue, 14 Jul 2020 08:47:50: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:47:50: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:47:50: #1 total tags in treatment: 23878029 INFO @ Tue, 14 Jul 2020 08:47:50: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:47:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:47:50: #1 tags after filtering in treatment: 21098808 INFO @ Tue, 14 Jul 2020 08:47:50: #1 Redundant rate of treatment: 0.12 INFO @ Tue, 14 Jul 2020 08:47:50: #1 finished! INFO @ Tue, 14 Jul 2020 08:47:50: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:47:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:47:51: 40000000 INFO @ Tue, 14 Jul 2020 08:47:51: #2 number of paired peaks: 44 WARNING @ Tue, 14 Jul 2020 08:47:51: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:47:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:47:55: 46000000 INFO @ Tue, 14 Jul 2020 08:47:58: 41000000 INFO @ Tue, 14 Jul 2020 08:48:01: 47000000 INFO @ Tue, 14 Jul 2020 08:48:04: 42000000 INFO @ Tue, 14 Jul 2020 08:48:07: 48000000 INFO @ Tue, 14 Jul 2020 08:48:11: 43000000 INFO @ Tue, 14 Jul 2020 08:48:14: 49000000 INFO @ Tue, 14 Jul 2020 08:48:15: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:48:15: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:48:15: #1 total tags in treatment: 23878029 INFO @ Tue, 14 Jul 2020 08:48:15: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:48:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:48:15: #1 tags after filtering in treatment: 21098808 INFO @ Tue, 14 Jul 2020 08:48:15: #1 Redundant rate of treatment: 0.12 INFO @ Tue, 14 Jul 2020 08:48:15: #1 finished! INFO @ Tue, 14 Jul 2020 08:48:15: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:48:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:48:17: #2 number of paired peaks: 44 WARNING @ Tue, 14 Jul 2020 08:48:17: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:48:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:48:18: 44000000 INFO @ Tue, 14 Jul 2020 08:48:24: 45000000 INFO @ Tue, 14 Jul 2020 08:48:30: 46000000 INFO @ Tue, 14 Jul 2020 08:48:36: 47000000 INFO @ Tue, 14 Jul 2020 08:48:42: 48000000 INFO @ Tue, 14 Jul 2020 08:48:48: 49000000 INFO @ Tue, 14 Jul 2020 08:48:49: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:48:49: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:48:49: #1 total tags in treatment: 23878029 INFO @ Tue, 14 Jul 2020 08:48:49: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:48:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:48:50: #1 tags after filtering in treatment: 21098808 INFO @ Tue, 14 Jul 2020 08:48:50: #1 Redundant rate of treatment: 0.12 INFO @ Tue, 14 Jul 2020 08:48:50: #1 finished! INFO @ Tue, 14 Jul 2020 08:48:50: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:48:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:48:51: #2 number of paired peaks: 44 WARNING @ Tue, 14 Jul 2020 08:48:51: Too few paired peaks (44) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:48:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736461/SRX5736461.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。