Job ID = 6626477 SRX = SRX5736460 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 21694959 spots for SRR8956872/SRR8956872.sra Written 21694959 spots for SRR8956872/SRR8956872.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626767 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:03:08 21694959 reads; of these: 21694959 (100.00%) were paired; of these: 4145286 (19.11%) aligned concordantly 0 times 9835584 (45.34%) aligned concordantly exactly 1 time 7714089 (35.56%) aligned concordantly >1 times ---- 4145286 pairs aligned concordantly 0 times; of these: 643067 (15.51%) aligned discordantly 1 time ---- 3502219 pairs aligned 0 times concordantly or discordantly; of these: 7004438 mates make up the pairs; of these: 5216502 (74.47%) aligned 0 times 495336 (7.07%) aligned exactly 1 time 1292600 (18.45%) aligned >1 times 87.98% overall alignment rate Time searching: 01:03:08 Overall time: 01:03:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1479115 / 18070887 = 0.0819 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:22:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:22:13: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:22:13: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:22:19: 1000000 INFO @ Tue, 14 Jul 2020 08:22:25: 2000000 INFO @ Tue, 14 Jul 2020 08:22:31: 3000000 INFO @ Tue, 14 Jul 2020 08:22:37: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:22:43: 5000000 INFO @ Tue, 14 Jul 2020 08:22:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:22:43: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:22:43: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:22:49: 1000000 INFO @ Tue, 14 Jul 2020 08:22:49: 6000000 INFO @ Tue, 14 Jul 2020 08:22:54: 2000000 INFO @ Tue, 14 Jul 2020 08:22:55: 7000000 INFO @ Tue, 14 Jul 2020 08:22:59: 3000000 INFO @ Tue, 14 Jul 2020 08:23:02: 8000000 INFO @ Tue, 14 Jul 2020 08:23:05: 4000000 INFO @ Tue, 14 Jul 2020 08:23:08: 9000000 INFO @ Tue, 14 Jul 2020 08:23:10: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:23:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:23:13: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:23:13: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:23:14: 10000000 INFO @ Tue, 14 Jul 2020 08:23:17: 6000000 INFO @ Tue, 14 Jul 2020 08:23:19: 1000000 INFO @ Tue, 14 Jul 2020 08:23:21: 11000000 INFO @ Tue, 14 Jul 2020 08:23:23: 7000000 INFO @ Tue, 14 Jul 2020 08:23:26: 2000000 INFO @ Tue, 14 Jul 2020 08:23:27: 12000000 INFO @ Tue, 14 Jul 2020 08:23:29: 8000000 INFO @ Tue, 14 Jul 2020 08:23:32: 3000000 INFO @ Tue, 14 Jul 2020 08:23:34: 13000000 INFO @ Tue, 14 Jul 2020 08:23:36: 9000000 INFO @ Tue, 14 Jul 2020 08:23:39: 4000000 INFO @ Tue, 14 Jul 2020 08:23:40: 14000000 INFO @ Tue, 14 Jul 2020 08:23:42: 10000000 INFO @ Tue, 14 Jul 2020 08:23:45: 5000000 INFO @ Tue, 14 Jul 2020 08:23:46: 15000000 INFO @ Tue, 14 Jul 2020 08:23:48: 11000000 INFO @ Tue, 14 Jul 2020 08:23:52: 6000000 INFO @ Tue, 14 Jul 2020 08:23:53: 16000000 INFO @ Tue, 14 Jul 2020 08:23:54: 12000000 INFO @ Tue, 14 Jul 2020 08:23:58: 7000000 INFO @ Tue, 14 Jul 2020 08:23:59: 17000000 INFO @ Tue, 14 Jul 2020 08:24:00: 13000000 INFO @ Tue, 14 Jul 2020 08:24:04: 8000000 INFO @ Tue, 14 Jul 2020 08:24:05: 18000000 INFO @ Tue, 14 Jul 2020 08:24:06: 14000000 INFO @ Tue, 14 Jul 2020 08:24:11: 9000000 INFO @ Tue, 14 Jul 2020 08:24:12: 19000000 INFO @ Tue, 14 Jul 2020 08:24:12: 15000000 INFO @ Tue, 14 Jul 2020 08:24:17: 10000000 INFO @ Tue, 14 Jul 2020 08:24:18: 20000000 INFO @ Tue, 14 Jul 2020 08:24:19: 16000000 INFO @ Tue, 14 Jul 2020 08:24:24: 11000000 INFO @ Tue, 14 Jul 2020 08:24:24: 21000000 INFO @ Tue, 14 Jul 2020 08:24:25: 17000000 INFO @ Tue, 14 Jul 2020 08:24:30: 12000000 INFO @ Tue, 14 Jul 2020 08:24:30: 22000000 INFO @ Tue, 14 Jul 2020 08:24:31: 18000000 INFO @ Tue, 14 Jul 2020 08:24:36: 23000000 INFO @ Tue, 14 Jul 2020 08:24:36: 13000000 INFO @ Tue, 14 Jul 2020 08:24:37: 19000000 INFO @ Tue, 14 Jul 2020 08:24:43: 24000000 INFO @ Tue, 14 Jul 2020 08:24:43: 14000000 INFO @ Tue, 14 Jul 2020 08:24:43: 20000000 INFO @ Tue, 14 Jul 2020 08:24:49: 25000000 INFO @ Tue, 14 Jul 2020 08:24:49: 21000000 INFO @ Tue, 14 Jul 2020 08:24:49: 15000000 INFO @ Tue, 14 Jul 2020 08:24:55: 26000000 INFO @ Tue, 14 Jul 2020 08:24:55: 22000000 INFO @ Tue, 14 Jul 2020 08:24:56: 16000000 INFO @ Tue, 14 Jul 2020 08:25:01: 23000000 INFO @ Tue, 14 Jul 2020 08:25:01: 27000000 INFO @ Tue, 14 Jul 2020 08:25:02: 17000000 INFO @ Tue, 14 Jul 2020 08:25:07: 24000000 INFO @ Tue, 14 Jul 2020 08:25:08: 28000000 INFO @ Tue, 14 Jul 2020 08:25:08: 18000000 INFO @ Tue, 14 Jul 2020 08:25:13: 25000000 INFO @ Tue, 14 Jul 2020 08:25:14: 29000000 INFO @ Tue, 14 Jul 2020 08:25:15: 19000000 INFO @ Tue, 14 Jul 2020 08:25:20: 26000000 INFO @ Tue, 14 Jul 2020 08:25:20: 30000000 INFO @ Tue, 14 Jul 2020 08:25:21: 20000000 INFO @ Tue, 14 Jul 2020 08:25:26: 27000000 INFO @ Tue, 14 Jul 2020 08:25:27: 31000000 INFO @ Tue, 14 Jul 2020 08:25:28: 21000000 INFO @ Tue, 14 Jul 2020 08:25:33: 28000000 INFO @ Tue, 14 Jul 2020 08:25:33: 32000000 INFO @ Tue, 14 Jul 2020 08:25:35: 22000000 INFO @ Tue, 14 Jul 2020 08:25:39: 33000000 INFO @ Tue, 14 Jul 2020 08:25:40: 29000000 INFO @ Tue, 14 Jul 2020 08:25:41: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:25:46: 34000000 INFO @ Tue, 14 Jul 2020 08:25:47: 30000000 INFO @ Tue, 14 Jul 2020 08:25:48: 24000000 INFO @ Tue, 14 Jul 2020 08:25:52: 35000000 INFO @ Tue, 14 Jul 2020 08:25:53: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:25:53: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:25:53: #1 total tags in treatment: 16078733 INFO @ Tue, 14 Jul 2020 08:25:53: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:25:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:25:53: 31000000 INFO @ Tue, 14 Jul 2020 08:25:54: #1 tags after filtering in treatment: 13871952 INFO @ Tue, 14 Jul 2020 08:25:54: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 14 Jul 2020 08:25:54: #1 finished! INFO @ Tue, 14 Jul 2020 08:25:54: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:25:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:25:55: #2 number of paired peaks: 95 WARNING @ Tue, 14 Jul 2020 08:25:55: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:25:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:25:55: 25000000 INFO @ Tue, 14 Jul 2020 08:26:00: 32000000 INFO @ Tue, 14 Jul 2020 08:26:01: 26000000 INFO @ Tue, 14 Jul 2020 08:26:06: 33000000 INFO @ Tue, 14 Jul 2020 08:26:08: 27000000 INFO @ Tue, 14 Jul 2020 08:26:13: 34000000 INFO @ Tue, 14 Jul 2020 08:26:14: 28000000 INFO @ Tue, 14 Jul 2020 08:26:19: 35000000 INFO @ Tue, 14 Jul 2020 08:26:21: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:26:21: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:26:21: #1 total tags in treatment: 16078733 INFO @ Tue, 14 Jul 2020 08:26:21: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:26:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:26:21: 29000000 INFO @ Tue, 14 Jul 2020 08:26:21: #1 tags after filtering in treatment: 13871952 INFO @ Tue, 14 Jul 2020 08:26:21: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 14 Jul 2020 08:26:21: #1 finished! INFO @ Tue, 14 Jul 2020 08:26:21: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:26:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:26:22: #2 number of paired peaks: 95 WARNING @ Tue, 14 Jul 2020 08:26:22: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:26:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:26:27: 30000000 INFO @ Tue, 14 Jul 2020 08:26:34: 31000000 INFO @ Tue, 14 Jul 2020 08:26:40: 32000000 INFO @ Tue, 14 Jul 2020 08:26:46: 33000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:26:53: 34000000 INFO @ Tue, 14 Jul 2020 08:26:59: 35000000 INFO @ Tue, 14 Jul 2020 08:27:00: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:27:00: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:27:00: #1 total tags in treatment: 16078733 INFO @ Tue, 14 Jul 2020 08:27:00: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:27:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:27:00: #1 tags after filtering in treatment: 13871952 INFO @ Tue, 14 Jul 2020 08:27:00: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 14 Jul 2020 08:27:00: #1 finished! INFO @ Tue, 14 Jul 2020 08:27:00: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:27:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:27:01: #2 number of paired peaks: 95 WARNING @ Tue, 14 Jul 2020 08:27:01: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:27:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736460/SRX5736460.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling