Job ID = 6528397 SRX = SRX5717041 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:07:39 prefetch.2.10.7: 1) Downloading 'SRR8936402'... 2020-06-29T15:07:39 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:10:39 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:10:39 prefetch.2.10.7: 1) 'SRR8936402' was downloaded successfully Read 31574552 spots for SRR8936402/SRR8936402.sra Written 31574552 spots for SRR8936402/SRR8936402.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:47 31574552 reads; of these: 31574552 (100.00%) were unpaired; of these: 4190513 (13.27%) aligned 0 times 17947115 (56.84%) aligned exactly 1 time 9436924 (29.89%) aligned >1 times 86.73% overall alignment rate Time searching: 00:10:47 Overall time: 00:10:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7307913 / 27384039 = 0.2669 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:36:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:36:23: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:36:23: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:36:29: 1000000 INFO @ Tue, 30 Jun 2020 00:36:36: 2000000 INFO @ Tue, 30 Jun 2020 00:36:42: 3000000 INFO @ Tue, 30 Jun 2020 00:36:48: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:36:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:36:53: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:36:53: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:36:54: 5000000 INFO @ Tue, 30 Jun 2020 00:37:00: 1000000 INFO @ Tue, 30 Jun 2020 00:37:01: 6000000 INFO @ Tue, 30 Jun 2020 00:37:07: 2000000 INFO @ Tue, 30 Jun 2020 00:37:08: 7000000 INFO @ Tue, 30 Jun 2020 00:37:14: 3000000 INFO @ Tue, 30 Jun 2020 00:37:15: 8000000 INFO @ Tue, 30 Jun 2020 00:37:21: 4000000 BedGraph に変換中... INFO @ Tue, 30 Jun 2020 00:37:21: 9000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:37:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:37:23: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:37:23: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:37:28: 5000000 INFO @ Tue, 30 Jun 2020 00:37:28: 10000000 INFO @ Tue, 30 Jun 2020 00:37:30: 1000000 INFO @ Tue, 30 Jun 2020 00:37:35: 6000000 INFO @ Tue, 30 Jun 2020 00:37:35: 11000000 INFO @ Tue, 30 Jun 2020 00:37:38: 2000000 INFO @ Tue, 30 Jun 2020 00:37:42: 7000000 INFO @ Tue, 30 Jun 2020 00:37:43: 12000000 INFO @ Tue, 30 Jun 2020 00:37:45: 3000000 INFO @ Tue, 30 Jun 2020 00:37:49: 8000000 INFO @ Tue, 30 Jun 2020 00:37:50: 13000000 INFO @ Tue, 30 Jun 2020 00:37:52: 4000000 INFO @ Tue, 30 Jun 2020 00:37:56: 9000000 INFO @ Tue, 30 Jun 2020 00:37:57: 14000000 INFO @ Tue, 30 Jun 2020 00:37:59: 5000000 INFO @ Tue, 30 Jun 2020 00:38:03: 10000000 INFO @ Tue, 30 Jun 2020 00:38:05: 15000000 INFO @ Tue, 30 Jun 2020 00:38:07: 6000000 INFO @ Tue, 30 Jun 2020 00:38:10: 11000000 INFO @ Tue, 30 Jun 2020 00:38:12: 16000000 INFO @ Tue, 30 Jun 2020 00:38:14: 7000000 INFO @ Tue, 30 Jun 2020 00:38:17: 12000000 INFO @ Tue, 30 Jun 2020 00:38:20: 17000000 INFO @ Tue, 30 Jun 2020 00:38:21: 8000000 INFO @ Tue, 30 Jun 2020 00:38:24: 13000000 INFO @ Tue, 30 Jun 2020 00:38:27: 18000000 INFO @ Tue, 30 Jun 2020 00:38:28: 9000000 INFO @ Tue, 30 Jun 2020 00:38:31: 14000000 INFO @ Tue, 30 Jun 2020 00:38:34: 19000000 INFO @ Tue, 30 Jun 2020 00:38:35: 10000000 INFO @ Tue, 30 Jun 2020 00:38:38: 15000000 INFO @ Tue, 30 Jun 2020 00:38:42: 20000000 INFO @ Tue, 30 Jun 2020 00:38:42: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:38:42: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:38:42: #1 total tags in treatment: 20076126 INFO @ Tue, 30 Jun 2020 00:38:42: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:38:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:38:42: 11000000 INFO @ Tue, 30 Jun 2020 00:38:43: #1 tags after filtering in treatment: 20076126 INFO @ Tue, 30 Jun 2020 00:38:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:38:43: #1 finished! INFO @ Tue, 30 Jun 2020 00:38:43: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:38:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:38:44: #2 number of paired peaks: 20 WARNING @ Tue, 30 Jun 2020 00:38:44: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:38:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:38:46: 16000000 INFO @ Tue, 30 Jun 2020 00:38:49: 12000000 INFO @ Tue, 30 Jun 2020 00:38:53: 17000000 INFO @ Tue, 30 Jun 2020 00:38:57: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:39:00: 18000000 INFO @ Tue, 30 Jun 2020 00:39:04: 14000000 INFO @ Tue, 30 Jun 2020 00:39:07: 19000000 INFO @ Tue, 30 Jun 2020 00:39:11: 15000000 INFO @ Tue, 30 Jun 2020 00:39:14: 20000000 INFO @ Tue, 30 Jun 2020 00:39:14: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:39:14: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:39:14: #1 total tags in treatment: 20076126 INFO @ Tue, 30 Jun 2020 00:39:14: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:39:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:39:15: #1 tags after filtering in treatment: 20076126 INFO @ Tue, 30 Jun 2020 00:39:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:39:15: #1 finished! INFO @ Tue, 30 Jun 2020 00:39:15: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:39:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:39:16: #2 number of paired peaks: 20 WARNING @ Tue, 30 Jun 2020 00:39:16: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:39:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 5 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:39:18: 16000000 INFO @ Tue, 30 Jun 2020 00:39:24: 17000000 INFO @ Tue, 30 Jun 2020 00:39:31: 18000000 INFO @ Tue, 30 Jun 2020 00:39:37: 19000000 INFO @ Tue, 30 Jun 2020 00:39:43: 20000000 INFO @ Tue, 30 Jun 2020 00:39:44: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:39:44: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:39:44: #1 total tags in treatment: 20076126 INFO @ Tue, 30 Jun 2020 00:39:44: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:39:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:39:44: #1 tags after filtering in treatment: 20076126 INFO @ Tue, 30 Jun 2020 00:39:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:39:44: #1 finished! INFO @ Tue, 30 Jun 2020 00:39:44: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:39:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:39:46: #2 number of paired peaks: 20 WARNING @ Tue, 30 Jun 2020 00:39:46: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:39:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5717041/SRX5717041.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。