Job ID = 14172374 SRX = SRX5681714 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10791076 spots for SRR8895589/SRR8895589.sra Written 10791076 spots for SRR8895589/SRR8895589.sra Read 5182403 spots for SRR8895590/SRR8895590.sra Written 5182403 spots for SRR8895590/SRR8895590.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172992 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:43:45 15973479 reads; of these: 15973479 (100.00%) were paired; of these: 1887122 (11.81%) aligned concordantly 0 times 11710213 (73.31%) aligned concordantly exactly 1 time 2376144 (14.88%) aligned concordantly >1 times ---- 1887122 pairs aligned concordantly 0 times; of these: 822152 (43.57%) aligned discordantly 1 time ---- 1064970 pairs aligned 0 times concordantly or discordantly; of these: 2129940 mates make up the pairs; of these: 1406476 (66.03%) aligned 0 times 376423 (17.67%) aligned exactly 1 time 347041 (16.29%) aligned >1 times 95.60% overall alignment rate Time searching: 00:43:45 Overall time: 00:43:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 605130 / 14815608 = 0.0408 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:08:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:08:20: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:08:20: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:08:29: 1000000 INFO @ Sat, 11 Dec 2021 16:08:39: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:08:48: 3000000 INFO @ Sat, 11 Dec 2021 16:08:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:08:50: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:08:50: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:08:58: 4000000 INFO @ Sat, 11 Dec 2021 16:09:00: 1000000 INFO @ Sat, 11 Dec 2021 16:09:08: 5000000 INFO @ Sat, 11 Dec 2021 16:09:10: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 16:09:18: 6000000 INFO @ Sat, 11 Dec 2021 16:09:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 16:09:20: #1 read tag files... INFO @ Sat, 11 Dec 2021 16:09:20: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 16:09:20: 3000000 INFO @ Sat, 11 Dec 2021 16:09:29: 1000000 INFO @ Sat, 11 Dec 2021 16:09:29: 7000000 INFO @ Sat, 11 Dec 2021 16:09:31: 4000000 INFO @ Sat, 11 Dec 2021 16:09:37: 2000000 INFO @ Sat, 11 Dec 2021 16:09:40: 8000000 INFO @ Sat, 11 Dec 2021 16:09:41: 5000000 INFO @ Sat, 11 Dec 2021 16:09:46: 3000000 INFO @ Sat, 11 Dec 2021 16:09:50: 9000000 INFO @ Sat, 11 Dec 2021 16:09:52: 6000000 INFO @ Sat, 11 Dec 2021 16:09:55: 4000000 INFO @ Sat, 11 Dec 2021 16:10:01: 10000000 INFO @ Sat, 11 Dec 2021 16:10:03: 7000000 INFO @ Sat, 11 Dec 2021 16:10:04: 5000000 INFO @ Sat, 11 Dec 2021 16:10:12: 11000000 INFO @ Sat, 11 Dec 2021 16:10:13: 6000000 INFO @ Sat, 11 Dec 2021 16:10:14: 8000000 INFO @ Sat, 11 Dec 2021 16:10:22: 7000000 INFO @ Sat, 11 Dec 2021 16:10:23: 12000000 INFO @ Sat, 11 Dec 2021 16:10:24: 9000000 INFO @ Sat, 11 Dec 2021 16:10:31: 8000000 INFO @ Sat, 11 Dec 2021 16:10:34: 13000000 INFO @ Sat, 11 Dec 2021 16:10:35: 10000000 INFO @ Sat, 11 Dec 2021 16:10:39: 9000000 INFO @ Sat, 11 Dec 2021 16:10:45: 14000000 INFO @ Sat, 11 Dec 2021 16:10:46: 11000000 INFO @ Sat, 11 Dec 2021 16:10:48: 10000000 INFO @ Sat, 11 Dec 2021 16:10:55: 15000000 INFO @ Sat, 11 Dec 2021 16:10:57: 11000000 INFO @ Sat, 11 Dec 2021 16:10:57: 12000000 INFO @ Sat, 11 Dec 2021 16:11:06: 12000000 INFO @ Sat, 11 Dec 2021 16:11:06: 16000000 INFO @ Sat, 11 Dec 2021 16:11:08: 13000000 INFO @ Sat, 11 Dec 2021 16:11:15: 13000000 INFO @ Sat, 11 Dec 2021 16:11:17: 17000000 INFO @ Sat, 11 Dec 2021 16:11:18: 14000000 INFO @ Sat, 11 Dec 2021 16:11:24: 14000000 INFO @ Sat, 11 Dec 2021 16:11:28: 18000000 INFO @ Sat, 11 Dec 2021 16:11:29: 15000000 INFO @ Sat, 11 Dec 2021 16:11:33: 15000000 INFO @ Sat, 11 Dec 2021 16:11:39: 16000000 INFO @ Sat, 11 Dec 2021 16:11:39: 19000000 INFO @ Sat, 11 Dec 2021 16:11:41: 16000000 INFO @ Sat, 11 Dec 2021 16:11:50: 17000000 INFO @ Sat, 11 Dec 2021 16:11:50: 17000000 INFO @ Sat, 11 Dec 2021 16:11:50: 20000000 INFO @ Sat, 11 Dec 2021 16:11:59: 18000000 INFO @ Sat, 11 Dec 2021 16:12:01: 18000000 INFO @ Sat, 11 Dec 2021 16:12:01: 21000000 INFO @ Sat, 11 Dec 2021 16:12:08: 19000000 INFO @ Sat, 11 Dec 2021 16:12:11: 19000000 INFO @ Sat, 11 Dec 2021 16:12:12: 22000000 INFO @ Sat, 11 Dec 2021 16:12:17: 20000000 INFO @ Sat, 11 Dec 2021 16:12:22: 20000000 INFO @ Sat, 11 Dec 2021 16:12:23: 23000000 INFO @ Sat, 11 Dec 2021 16:12:26: 21000000 INFO @ Sat, 11 Dec 2021 16:12:33: 21000000 INFO @ Sat, 11 Dec 2021 16:12:34: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 16:12:35: 22000000 INFO @ Sat, 11 Dec 2021 16:12:43: 23000000 INFO @ Sat, 11 Dec 2021 16:12:44: 22000000 INFO @ Sat, 11 Dec 2021 16:12:45: 25000000 INFO @ Sat, 11 Dec 2021 16:12:53: 24000000 INFO @ Sat, 11 Dec 2021 16:12:54: 23000000 INFO @ Sat, 11 Dec 2021 16:12:56: 26000000 INFO @ Sat, 11 Dec 2021 16:13:01: 25000000 INFO @ Sat, 11 Dec 2021 16:13:05: 24000000 INFO @ Sat, 11 Dec 2021 16:13:06: 27000000 INFO @ Sat, 11 Dec 2021 16:13:10: 26000000 INFO @ Sat, 11 Dec 2021 16:13:16: 25000000 INFO @ Sat, 11 Dec 2021 16:13:17: 28000000 INFO @ Sat, 11 Dec 2021 16:13:19: 27000000 INFO @ Sat, 11 Dec 2021 16:13:27: 26000000 INFO @ Sat, 11 Dec 2021 16:13:28: 28000000 INFO @ Sat, 11 Dec 2021 16:13:28: 29000000 INFO @ Sat, 11 Dec 2021 16:13:31: #1 tag size is determined as 100 bps INFO @ Sat, 11 Dec 2021 16:13:31: #1 tag size = 100 INFO @ Sat, 11 Dec 2021 16:13:31: #1 total tags in treatment: 13504985 INFO @ Sat, 11 Dec 2021 16:13:31: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:13:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:13:32: #1 tags after filtering in treatment: 12294940 INFO @ Sat, 11 Dec 2021 16:13:32: #1 Redundant rate of treatment: 0.09 INFO @ Sat, 11 Dec 2021 16:13:32: #1 finished! INFO @ Sat, 11 Dec 2021 16:13:32: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:13:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:13:32: #2 number of paired peaks: 2 WARNING @ Sat, 11 Dec 2021 16:13:32: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 16:13:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 16:13:35: 29000000 INFO @ Sat, 11 Dec 2021 16:13:37: 27000000 INFO @ Sat, 11 Dec 2021 16:13:38: #1 tag size is determined as 100 bps INFO @ Sat, 11 Dec 2021 16:13:38: #1 tag size = 100 INFO @ Sat, 11 Dec 2021 16:13:38: #1 total tags in treatment: 13504985 INFO @ Sat, 11 Dec 2021 16:13:38: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:13:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:13:38: #1 tags after filtering in treatment: 12294940 INFO @ Sat, 11 Dec 2021 16:13:38: #1 Redundant rate of treatment: 0.09 INFO @ Sat, 11 Dec 2021 16:13:38: #1 finished! INFO @ Sat, 11 Dec 2021 16:13:38: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:13:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:13:39: #2 number of paired peaks: 2 WARNING @ Sat, 11 Dec 2021 16:13:39: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 16:13:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 16:13:47: 28000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 16:13:56: 29000000 INFO @ Sat, 11 Dec 2021 16:13:59: #1 tag size is determined as 100 bps INFO @ Sat, 11 Dec 2021 16:13:59: #1 tag size = 100 INFO @ Sat, 11 Dec 2021 16:13:59: #1 total tags in treatment: 13504985 INFO @ Sat, 11 Dec 2021 16:13:59: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 16:13:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 16:14:00: #1 tags after filtering in treatment: 12294940 INFO @ Sat, 11 Dec 2021 16:14:00: #1 Redundant rate of treatment: 0.09 INFO @ Sat, 11 Dec 2021 16:14:00: #1 finished! INFO @ Sat, 11 Dec 2021 16:14:00: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 16:14:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 16:14:00: #2 number of paired peaks: 2 WARNING @ Sat, 11 Dec 2021 16:14:00: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 16:14:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling