Job ID = 10165826 SRX = SRX5681714 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10791076 spots for SRR8895589/SRR8895589.sra Written 10791076 spots for SRR8895589/SRR8895589.sra Read 5182403 spots for SRR8895590/SRR8895590.sra Written 5182403 spots for SRR8895590/SRR8895590.sra fastq に変換しました。 bowtie でマッピング中... Your job 10166407 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:32:36 15973479 reads; of these: 15973479 (100.00%) were paired; of these: 1887122 (11.81%) aligned concordantly 0 times 11710213 (73.31%) aligned concordantly exactly 1 time 2376144 (14.88%) aligned concordantly >1 times ---- 1887122 pairs aligned concordantly 0 times; of these: 822152 (43.57%) aligned discordantly 1 time ---- 1064970 pairs aligned 0 times concordantly or discordantly; of these: 2129940 mates make up the pairs; of these: 1406476 (66.03%) aligned 0 times 376423 (17.67%) aligned exactly 1 time 347041 (16.29%) aligned >1 times 95.60% overall alignment rate Time searching: 00:32:36 Overall time: 00:32:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 605130 / 14815608 = 0.0408 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:40:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:40:50: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:40:50: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:40:57: 1000000 INFO @ Thu, 08 Oct 2020 20:41:04: 2000000 INFO @ Thu, 08 Oct 2020 20:41:11: 3000000 INFO @ Thu, 08 Oct 2020 20:41:18: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:41:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:41:20: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:41:20: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:41:26: 5000000 INFO @ Thu, 08 Oct 2020 20:41:29: 1000000 INFO @ Thu, 08 Oct 2020 20:41:34: 6000000 INFO @ Thu, 08 Oct 2020 20:41:37: 2000000 INFO @ Thu, 08 Oct 2020 20:41:41: 7000000 INFO @ Thu, 08 Oct 2020 20:41:45: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:41:49: 8000000 INFO @ Thu, 08 Oct 2020 20:41:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:41:50: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:41:50: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:41:53: 4000000 INFO @ Thu, 08 Oct 2020 20:41:57: 9000000 INFO @ Thu, 08 Oct 2020 20:41:59: 1000000 INFO @ Thu, 08 Oct 2020 20:42:01: 5000000 INFO @ Thu, 08 Oct 2020 20:42:05: 10000000 INFO @ Thu, 08 Oct 2020 20:42:07: 2000000 INFO @ Thu, 08 Oct 2020 20:42:09: 6000000 INFO @ Thu, 08 Oct 2020 20:42:13: 11000000 INFO @ Thu, 08 Oct 2020 20:42:15: 3000000 INFO @ Thu, 08 Oct 2020 20:42:17: 7000000 INFO @ Thu, 08 Oct 2020 20:42:21: 12000000 INFO @ Thu, 08 Oct 2020 20:42:23: 4000000 INFO @ Thu, 08 Oct 2020 20:42:25: 8000000 INFO @ Thu, 08 Oct 2020 20:42:29: 13000000 INFO @ Thu, 08 Oct 2020 20:42:31: 5000000 INFO @ Thu, 08 Oct 2020 20:42:33: 9000000 INFO @ Thu, 08 Oct 2020 20:42:37: 14000000 INFO @ Thu, 08 Oct 2020 20:42:39: 6000000 INFO @ Thu, 08 Oct 2020 20:42:41: 10000000 INFO @ Thu, 08 Oct 2020 20:42:45: 15000000 INFO @ Thu, 08 Oct 2020 20:42:47: 7000000 INFO @ Thu, 08 Oct 2020 20:42:49: 11000000 INFO @ Thu, 08 Oct 2020 20:42:53: 16000000 INFO @ Thu, 08 Oct 2020 20:42:55: 8000000 INFO @ Thu, 08 Oct 2020 20:42:57: 12000000 INFO @ Thu, 08 Oct 2020 20:43:00: 17000000 INFO @ Thu, 08 Oct 2020 20:43:03: 9000000 INFO @ Thu, 08 Oct 2020 20:43:05: 13000000 INFO @ Thu, 08 Oct 2020 20:43:08: 18000000 INFO @ Thu, 08 Oct 2020 20:43:12: 10000000 INFO @ Thu, 08 Oct 2020 20:43:13: 14000000 INFO @ Thu, 08 Oct 2020 20:43:16: 19000000 INFO @ Thu, 08 Oct 2020 20:43:19: 11000000 INFO @ Thu, 08 Oct 2020 20:43:21: 15000000 INFO @ Thu, 08 Oct 2020 20:43:24: 20000000 INFO @ Thu, 08 Oct 2020 20:43:28: 12000000 INFO @ Thu, 08 Oct 2020 20:43:29: 16000000 INFO @ Thu, 08 Oct 2020 20:43:32: 21000000 INFO @ Thu, 08 Oct 2020 20:43:36: 13000000 INFO @ Thu, 08 Oct 2020 20:43:37: 17000000 INFO @ Thu, 08 Oct 2020 20:43:40: 22000000 INFO @ Thu, 08 Oct 2020 20:43:44: 14000000 INFO @ Thu, 08 Oct 2020 20:43:46: 18000000 INFO @ Thu, 08 Oct 2020 20:43:48: 23000000 INFO @ Thu, 08 Oct 2020 20:43:52: 15000000 INFO @ Thu, 08 Oct 2020 20:43:54: 19000000 INFO @ Thu, 08 Oct 2020 20:43:56: 24000000 INFO @ Thu, 08 Oct 2020 20:44:00: 16000000 INFO @ Thu, 08 Oct 2020 20:44:02: 20000000 INFO @ Thu, 08 Oct 2020 20:44:04: 25000000 INFO @ Thu, 08 Oct 2020 20:44:08: 17000000 INFO @ Thu, 08 Oct 2020 20:44:10: 21000000 INFO @ Thu, 08 Oct 2020 20:44:12: 26000000 INFO @ Thu, 08 Oct 2020 20:44:16: 18000000 INFO @ Thu, 08 Oct 2020 20:44:18: 22000000 INFO @ Thu, 08 Oct 2020 20:44:19: 27000000 INFO @ Thu, 08 Oct 2020 20:44:24: 19000000 INFO @ Thu, 08 Oct 2020 20:44:26: 23000000 INFO @ Thu, 08 Oct 2020 20:44:27: 28000000 INFO @ Thu, 08 Oct 2020 20:44:32: 20000000 INFO @ Thu, 08 Oct 2020 20:44:34: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:44:35: 29000000 INFO @ Thu, 08 Oct 2020 20:44:38: #1 tag size is determined as 100 bps INFO @ Thu, 08 Oct 2020 20:44:38: #1 tag size = 100 INFO @ Thu, 08 Oct 2020 20:44:38: #1 total tags in treatment: 13504985 INFO @ Thu, 08 Oct 2020 20:44:38: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:44:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:44:38: #1 tags after filtering in treatment: 12294943 INFO @ Thu, 08 Oct 2020 20:44:38: #1 Redundant rate of treatment: 0.09 INFO @ Thu, 08 Oct 2020 20:44:38: #1 finished! INFO @ Thu, 08 Oct 2020 20:44:38: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:44:38: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:44:39: #2 number of paired peaks: 2 WARNING @ Thu, 08 Oct 2020 20:44:39: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:44:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:44:40: 21000000 INFO @ Thu, 08 Oct 2020 20:44:42: 25000000 INFO @ Thu, 08 Oct 2020 20:44:47: 22000000 INFO @ Thu, 08 Oct 2020 20:44:50: 26000000 INFO @ Thu, 08 Oct 2020 20:44:55: 23000000 INFO @ Thu, 08 Oct 2020 20:44:57: 27000000 INFO @ Thu, 08 Oct 2020 20:45:03: 24000000 INFO @ Thu, 08 Oct 2020 20:45:05: 28000000 INFO @ Thu, 08 Oct 2020 20:45:11: 25000000 INFO @ Thu, 08 Oct 2020 20:45:13: 29000000 INFO @ Thu, 08 Oct 2020 20:45:16: #1 tag size is determined as 100 bps INFO @ Thu, 08 Oct 2020 20:45:16: #1 tag size = 100 INFO @ Thu, 08 Oct 2020 20:45:16: #1 total tags in treatment: 13504985 INFO @ Thu, 08 Oct 2020 20:45:16: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:45:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:45:16: #1 tags after filtering in treatment: 12294943 INFO @ Thu, 08 Oct 2020 20:45:16: #1 Redundant rate of treatment: 0.09 INFO @ Thu, 08 Oct 2020 20:45:16: #1 finished! INFO @ Thu, 08 Oct 2020 20:45:16: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:45:16: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:45:17: #2 number of paired peaks: 2 WARNING @ Thu, 08 Oct 2020 20:45:17: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:45:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:45:18: 26000000 INFO @ Thu, 08 Oct 2020 20:45:26: 27000000 INFO @ Thu, 08 Oct 2020 20:45:33: 28000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 20:45:39: 29000000 INFO @ Thu, 08 Oct 2020 20:45:42: #1 tag size is determined as 100 bps INFO @ Thu, 08 Oct 2020 20:45:42: #1 tag size = 100 INFO @ Thu, 08 Oct 2020 20:45:42: #1 total tags in treatment: 13504985 INFO @ Thu, 08 Oct 2020 20:45:42: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:45:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:45:42: #1 tags after filtering in treatment: 12294943 INFO @ Thu, 08 Oct 2020 20:45:42: #1 Redundant rate of treatment: 0.09 INFO @ Thu, 08 Oct 2020 20:45:42: #1 finished! INFO @ Thu, 08 Oct 2020 20:45:42: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:45:42: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:45:43: #2 number of paired peaks: 2 WARNING @ Thu, 08 Oct 2020 20:45:43: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:45:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681714/SRX5681714.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling