Job ID = 14172339 SRX = SRX5681710 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9738101 spots for SRR8895581/SRR8895581.sra Written 9738101 spots for SRR8895581/SRR8895581.sra 2021-12-11T06:10:13 fastq-dump.2.10.7 fatal: SIGNAL - Segmentation fault fastq-dump quit with error code 1 fastq に変換しました。 bowtie でマッピング中... Your job 14172927 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Error: Read SRR8895582.1064175 1064175 length=100 has more quality values than read characters. terminate called after throwing an instance of 'int' (ERR): bowtie2-align died with signal 6 (ABRT) (core dumped) マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 440086 / 10063034 = 0.0437 in library ' ' awk: cmd. line:1: (FILENAME=- FNR=1) fatal: division by zero attempted BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:51:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:51:27: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:51:27: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:51:37: 1000000 INFO @ Sat, 11 Dec 2021 15:51:47: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:51:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:51:56: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:51:56: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:51:58: 3000000 INFO @ Sat, 11 Dec 2021 15:52:07: 1000000 INFO @ Sat, 11 Dec 2021 15:52:08: 4000000 INFO @ Sat, 11 Dec 2021 15:52:18: 2000000 INFO @ Sat, 11 Dec 2021 15:52:19: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:52:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:52:26: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:52:26: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:52:29: 3000000 INFO @ Sat, 11 Dec 2021 15:52:30: 6000000 INFO @ Sat, 11 Dec 2021 15:52:40: 1000000 INFO @ Sat, 11 Dec 2021 15:52:40: 7000000 INFO @ Sat, 11 Dec 2021 15:52:41: 4000000 INFO @ Sat, 11 Dec 2021 15:52:51: 8000000 INFO @ Sat, 11 Dec 2021 15:52:52: 5000000 INFO @ Sat, 11 Dec 2021 15:52:54: 2000000 INFO @ Sat, 11 Dec 2021 15:53:02: 9000000 INFO @ Sat, 11 Dec 2021 15:53:04: 6000000 INFO @ Sat, 11 Dec 2021 15:53:08: 3000000 INFO @ Sat, 11 Dec 2021 15:53:13: 10000000 INFO @ Sat, 11 Dec 2021 15:53:16: 7000000 INFO @ Sat, 11 Dec 2021 15:53:22: 4000000 INFO @ Sat, 11 Dec 2021 15:53:24: 11000000 INFO @ Sat, 11 Dec 2021 15:53:27: 8000000 INFO @ Sat, 11 Dec 2021 15:53:35: 5000000 INFO @ Sat, 11 Dec 2021 15:53:35: 12000000 INFO @ Sat, 11 Dec 2021 15:53:39: 9000000 INFO @ Sat, 11 Dec 2021 15:53:47: 13000000 INFO @ Sat, 11 Dec 2021 15:53:48: 6000000 INFO @ Sat, 11 Dec 2021 15:53:51: 10000000 INFO @ Sat, 11 Dec 2021 15:53:58: 14000000 INFO @ Sat, 11 Dec 2021 15:54:02: 7000000 INFO @ Sat, 11 Dec 2021 15:54:03: 11000000 INFO @ Sat, 11 Dec 2021 15:54:09: 15000000 INFO @ Sat, 11 Dec 2021 15:54:15: 12000000 INFO @ Sat, 11 Dec 2021 15:54:15: 8000000 INFO @ Sat, 11 Dec 2021 15:54:21: 16000000 INFO @ Sat, 11 Dec 2021 15:54:26: 13000000 INFO @ Sat, 11 Dec 2021 15:54:28: 9000000 INFO @ Sat, 11 Dec 2021 15:54:32: 17000000 INFO @ Sat, 11 Dec 2021 15:54:38: 14000000 INFO @ Sat, 11 Dec 2021 15:54:41: 10000000 INFO @ Sat, 11 Dec 2021 15:54:44: 18000000 INFO @ Sat, 11 Dec 2021 15:54:50: 15000000 INFO @ Sat, 11 Dec 2021 15:54:54: 11000000 INFO @ Sat, 11 Dec 2021 15:54:55: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 15:55:01: 16000000 INFO @ Sat, 11 Dec 2021 15:55:06: #1 tag size is determined as 100 bps INFO @ Sat, 11 Dec 2021 15:55:06: #1 tag size = 100 INFO @ Sat, 11 Dec 2021 15:55:06: #1 total tags in treatment: 9235654 INFO @ Sat, 11 Dec 2021 15:55:06: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:55:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:55:06: #1 tags after filtering in treatment: 8585654 INFO @ Sat, 11 Dec 2021 15:55:06: #1 Redundant rate of treatment: 0.07 INFO @ Sat, 11 Dec 2021 15:55:06: #1 finished! INFO @ Sat, 11 Dec 2021 15:55:06: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:55:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:55:07: #2 number of paired peaks: 24 WARNING @ Sat, 11 Dec 2021 15:55:07: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 15:55:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:55:07: 12000000 INFO @ Sat, 11 Dec 2021 15:55:12: 17000000 INFO @ Sat, 11 Dec 2021 15:55:20: 13000000 INFO @ Sat, 11 Dec 2021 15:55:22: 18000000 INFO @ Sat, 11 Dec 2021 15:55:33: 14000000 INFO @ Sat, 11 Dec 2021 15:55:33: 19000000 INFO @ Sat, 11 Dec 2021 15:55:42: #1 tag size is determined as 100 bps INFO @ Sat, 11 Dec 2021 15:55:42: #1 tag size = 100 INFO @ Sat, 11 Dec 2021 15:55:42: #1 total tags in treatment: 9235654 INFO @ Sat, 11 Dec 2021 15:55:42: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:55:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:55:42: #1 tags after filtering in treatment: 8585654 INFO @ Sat, 11 Dec 2021 15:55:42: #1 Redundant rate of treatment: 0.07 INFO @ Sat, 11 Dec 2021 15:55:42: #1 finished! INFO @ Sat, 11 Dec 2021 15:55:42: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:55:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:55:43: #2 number of paired peaks: 24 WARNING @ Sat, 11 Dec 2021 15:55:43: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 15:55:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:55:46: 15000000 INFO @ Sat, 11 Dec 2021 15:55:59: 16000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 15:56:12: 17000000 INFO @ Sat, 11 Dec 2021 15:56:24: 18000000 INFO @ Sat, 11 Dec 2021 15:56:37: 19000000 INFO @ Sat, 11 Dec 2021 15:56:48: #1 tag size is determined as 100 bps INFO @ Sat, 11 Dec 2021 15:56:48: #1 tag size = 100 INFO @ Sat, 11 Dec 2021 15:56:48: #1 total tags in treatment: 9235654 INFO @ Sat, 11 Dec 2021 15:56:48: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:56:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:56:48: #1 tags after filtering in treatment: 8585654 INFO @ Sat, 11 Dec 2021 15:56:48: #1 Redundant rate of treatment: 0.07 INFO @ Sat, 11 Dec 2021 15:56:48: #1 finished! INFO @ Sat, 11 Dec 2021 15:56:48: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:56:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:56:49: #2 number of paired peaks: 24 WARNING @ Sat, 11 Dec 2021 15:56:49: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 15:56:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling