Job ID = 10165817 SRX = SRX5681710 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9738101 spots for SRR8895581/SRR8895581.sra Written 9738101 spots for SRR8895581/SRR8895581.sra 2020-10-08T10:57:14 fastq-dump.2.10.7 fatal: SIGNAL - Segmentation fault fastq-dump quit with error code 1 fastq に変換しました。 bowtie でマッピング中... Your job 10166377 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Error: Read SRR8895582.1064175 1064175 length=100 has more quality values than read characters. terminate called after throwing an instance of 'int' (ERR): bowtie2-align died with signal 6 (ABRT) (core dumped) マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 440086 / 10063034 = 0.0437 in library ' ' awk: cmd. line:1: (FILENAME=- FNR=1) fatal: division by zero attempted BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:35:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:35:17: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:35:17: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:35:24: 1000000 INFO @ Thu, 08 Oct 2020 20:35:30: 2000000 INFO @ Thu, 08 Oct 2020 20:35:36: 3000000 INFO @ Thu, 08 Oct 2020 20:35:43: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:35:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:35:46: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:35:46: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:35:49: 5000000 INFO @ Thu, 08 Oct 2020 20:35:53: 1000000 INFO @ Thu, 08 Oct 2020 20:35:56: 6000000 INFO @ Thu, 08 Oct 2020 20:36:00: 2000000 INFO @ Thu, 08 Oct 2020 20:36:03: 7000000 INFO @ Thu, 08 Oct 2020 20:36:06: 3000000 INFO @ Thu, 08 Oct 2020 20:36:10: 8000000 INFO @ Thu, 08 Oct 2020 20:36:13: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:36:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:36:16: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:36:16: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:36:17: 9000000 INFO @ Thu, 08 Oct 2020 20:36:21: 5000000 INFO @ Thu, 08 Oct 2020 20:36:24: 1000000 INFO @ Thu, 08 Oct 2020 20:36:25: 10000000 INFO @ Thu, 08 Oct 2020 20:36:29: 6000000 INFO @ Thu, 08 Oct 2020 20:36:32: 2000000 INFO @ Thu, 08 Oct 2020 20:36:32: 11000000 INFO @ Thu, 08 Oct 2020 20:36:37: 7000000 INFO @ Thu, 08 Oct 2020 20:36:39: 3000000 INFO @ Thu, 08 Oct 2020 20:36:40: 12000000 INFO @ Thu, 08 Oct 2020 20:36:45: 8000000 INFO @ Thu, 08 Oct 2020 20:36:47: 4000000 INFO @ Thu, 08 Oct 2020 20:36:48: 13000000 INFO @ Thu, 08 Oct 2020 20:36:53: 9000000 INFO @ Thu, 08 Oct 2020 20:36:55: 5000000 INFO @ Thu, 08 Oct 2020 20:36:56: 14000000 INFO @ Thu, 08 Oct 2020 20:37:01: 10000000 INFO @ Thu, 08 Oct 2020 20:37:02: 6000000 INFO @ Thu, 08 Oct 2020 20:37:04: 15000000 INFO @ Thu, 08 Oct 2020 20:37:09: 11000000 INFO @ Thu, 08 Oct 2020 20:37:10: 7000000 INFO @ Thu, 08 Oct 2020 20:37:12: 16000000 INFO @ Thu, 08 Oct 2020 20:37:16: 12000000 INFO @ Thu, 08 Oct 2020 20:37:18: 8000000 INFO @ Thu, 08 Oct 2020 20:37:20: 17000000 INFO @ Thu, 08 Oct 2020 20:37:24: 13000000 INFO @ Thu, 08 Oct 2020 20:37:25: 9000000 INFO @ Thu, 08 Oct 2020 20:37:28: 18000000 INFO @ Thu, 08 Oct 2020 20:37:32: 14000000 INFO @ Thu, 08 Oct 2020 20:37:33: 10000000 INFO @ Thu, 08 Oct 2020 20:37:36: 19000000 INFO @ Thu, 08 Oct 2020 20:37:40: 15000000 INFO @ Thu, 08 Oct 2020 20:37:41: 11000000 INFO @ Thu, 08 Oct 2020 20:37:42: #1 tag size is determined as 100 bps INFO @ Thu, 08 Oct 2020 20:37:42: #1 tag size = 100 INFO @ Thu, 08 Oct 2020 20:37:42: #1 total tags in treatment: 9235654 INFO @ Thu, 08 Oct 2020 20:37:42: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:37:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:37:43: #1 tags after filtering in treatment: 8585654 INFO @ Thu, 08 Oct 2020 20:37:43: #1 Redundant rate of treatment: 0.07 INFO @ Thu, 08 Oct 2020 20:37:43: #1 finished! INFO @ Thu, 08 Oct 2020 20:37:43: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:37:43: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:37:43: #2 number of paired peaks: 24 WARNING @ Thu, 08 Oct 2020 20:37:43: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:37:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:37:47: 16000000 INFO @ Thu, 08 Oct 2020 20:37:47: 12000000 INFO @ Thu, 08 Oct 2020 20:37:54: 13000000 INFO @ Thu, 08 Oct 2020 20:37:54: 17000000 INFO @ Thu, 08 Oct 2020 20:38:01: 14000000 INFO @ Thu, 08 Oct 2020 20:38:01: 18000000 INFO @ Thu, 08 Oct 2020 20:38:07: 15000000 INFO @ Thu, 08 Oct 2020 20:38:08: 19000000 INFO @ Thu, 08 Oct 2020 20:38:14: 16000000 INFO @ Thu, 08 Oct 2020 20:38:15: #1 tag size is determined as 100 bps INFO @ Thu, 08 Oct 2020 20:38:15: #1 tag size = 100 INFO @ Thu, 08 Oct 2020 20:38:15: #1 total tags in treatment: 9235654 INFO @ Thu, 08 Oct 2020 20:38:15: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:38:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:38:15: #1 tags after filtering in treatment: 8585654 INFO @ Thu, 08 Oct 2020 20:38:15: #1 Redundant rate of treatment: 0.07 INFO @ Thu, 08 Oct 2020 20:38:15: #1 finished! INFO @ Thu, 08 Oct 2020 20:38:15: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:38:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:38:15: #2 number of paired peaks: 24 WARNING @ Thu, 08 Oct 2020 20:38:15: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:38:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:38:20: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:38:26: 18000000 INFO @ Thu, 08 Oct 2020 20:38:33: 19000000 INFO @ Thu, 08 Oct 2020 20:38:38: #1 tag size is determined as 100 bps INFO @ Thu, 08 Oct 2020 20:38:38: #1 tag size = 100 INFO @ Thu, 08 Oct 2020 20:38:38: #1 total tags in treatment: 9235654 INFO @ Thu, 08 Oct 2020 20:38:38: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:38:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:38:38: #1 tags after filtering in treatment: 8585654 INFO @ Thu, 08 Oct 2020 20:38:38: #1 Redundant rate of treatment: 0.07 INFO @ Thu, 08 Oct 2020 20:38:38: #1 finished! INFO @ Thu, 08 Oct 2020 20:38:38: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:38:38: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:38:39: #2 number of paired peaks: 24 WARNING @ Thu, 08 Oct 2020 20:38:39: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:38:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681710/SRX5681710.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。