Job ID = 10165807 SRX = SRX5681703 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 28479118 spots for SRR8895565/SRR8895565.sra Written 28479118 spots for SRR8895565/SRR8895565.sra Read 28683016 spots for SRR8895566/SRR8895566.sra Written 28683016 spots for SRR8895566/SRR8895566.sra Read 24227461 spots for SRR8895567/SRR8895567.sra Written 24227461 spots for SRR8895567/SRR8895567.sra fastq に変換しました。 bowtie でマッピング中... Your job 10167009 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 01:29:24 81389595 reads; of these: 81389595 (100.00%) were paired; of these: 32216861 (39.58%) aligned concordantly 0 times 42112219 (51.74%) aligned concordantly exactly 1 time 7060515 (8.67%) aligned concordantly >1 times ---- 32216861 pairs aligned concordantly 0 times; of these: 1298070 (4.03%) aligned discordantly 1 time ---- 30918791 pairs aligned 0 times concordantly or discordantly; of these: 61837582 mates make up the pairs; of these: 57751194 (93.39%) aligned 0 times 2667202 (4.31%) aligned exactly 1 time 1419186 (2.30%) aligned >1 times 64.52% overall alignment rate Time searching: 01:29:25 Overall time: 01:29:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 15749400 / 50222717 = 0.3136 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:58:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:58:36: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:58:36: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:58:43: 1000000 INFO @ Thu, 08 Oct 2020 21:58:50: 2000000 INFO @ Thu, 08 Oct 2020 21:58:57: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:59:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:59:04: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:59:04: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:59:04: 4000000 INFO @ Thu, 08 Oct 2020 21:59:11: 1000000 INFO @ Thu, 08 Oct 2020 21:59:12: 5000000 INFO @ Thu, 08 Oct 2020 21:59:18: 2000000 INFO @ Thu, 08 Oct 2020 21:59:20: 6000000 INFO @ Thu, 08 Oct 2020 21:59:25: 3000000 INFO @ Thu, 08 Oct 2020 21:59:28: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 21:59:32: 4000000 INFO @ Thu, 08 Oct 2020 21:59:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 21:59:35: #1 read tag files... INFO @ Thu, 08 Oct 2020 21:59:35: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 21:59:36: 8000000 INFO @ Thu, 08 Oct 2020 21:59:40: 5000000 INFO @ Thu, 08 Oct 2020 21:59:42: 1000000 INFO @ Thu, 08 Oct 2020 21:59:44: 9000000 INFO @ Thu, 08 Oct 2020 21:59:47: 6000000 INFO @ Thu, 08 Oct 2020 21:59:50: 2000000 INFO @ Thu, 08 Oct 2020 21:59:52: 10000000 INFO @ Thu, 08 Oct 2020 21:59:54: 7000000 INFO @ Thu, 08 Oct 2020 21:59:57: 3000000 INFO @ Thu, 08 Oct 2020 21:59:59: 11000000 INFO @ Thu, 08 Oct 2020 22:00:02: 8000000 INFO @ Thu, 08 Oct 2020 22:00:05: 4000000 INFO @ Thu, 08 Oct 2020 22:00:07: 12000000 INFO @ Thu, 08 Oct 2020 22:00:09: 9000000 INFO @ Thu, 08 Oct 2020 22:00:12: 5000000 INFO @ Thu, 08 Oct 2020 22:00:15: 13000000 INFO @ Thu, 08 Oct 2020 22:00:17: 10000000 INFO @ Thu, 08 Oct 2020 22:00:20: 6000000 INFO @ Thu, 08 Oct 2020 22:00:22: 14000000 INFO @ Thu, 08 Oct 2020 22:00:24: 11000000 INFO @ Thu, 08 Oct 2020 22:00:28: 7000000 INFO @ Thu, 08 Oct 2020 22:00:30: 15000000 INFO @ Thu, 08 Oct 2020 22:00:32: 12000000 INFO @ Thu, 08 Oct 2020 22:00:35: 8000000 INFO @ Thu, 08 Oct 2020 22:00:38: 16000000 INFO @ Thu, 08 Oct 2020 22:00:39: 13000000 INFO @ Thu, 08 Oct 2020 22:00:42: 9000000 INFO @ Thu, 08 Oct 2020 22:00:45: 17000000 INFO @ Thu, 08 Oct 2020 22:00:47: 14000000 INFO @ Thu, 08 Oct 2020 22:00:50: 10000000 INFO @ Thu, 08 Oct 2020 22:00:53: 18000000 INFO @ Thu, 08 Oct 2020 22:00:54: 15000000 INFO @ Thu, 08 Oct 2020 22:00:57: 11000000 INFO @ Thu, 08 Oct 2020 22:01:01: 19000000 INFO @ Thu, 08 Oct 2020 22:01:02: 16000000 INFO @ Thu, 08 Oct 2020 22:01:04: 12000000 INFO @ Thu, 08 Oct 2020 22:01:08: 20000000 INFO @ Thu, 08 Oct 2020 22:01:09: 17000000 INFO @ Thu, 08 Oct 2020 22:01:12: 13000000 INFO @ Thu, 08 Oct 2020 22:01:16: 21000000 INFO @ Thu, 08 Oct 2020 22:01:16: 18000000 INFO @ Thu, 08 Oct 2020 22:01:19: 14000000 INFO @ Thu, 08 Oct 2020 22:01:24: 19000000 INFO @ Thu, 08 Oct 2020 22:01:24: 22000000 INFO @ Thu, 08 Oct 2020 22:01:26: 15000000 INFO @ Thu, 08 Oct 2020 22:01:31: 20000000 INFO @ Thu, 08 Oct 2020 22:01:32: 23000000 INFO @ Thu, 08 Oct 2020 22:01:34: 16000000 INFO @ Thu, 08 Oct 2020 22:01:38: 21000000 INFO @ Thu, 08 Oct 2020 22:01:40: 24000000 INFO @ Thu, 08 Oct 2020 22:01:41: 17000000 INFO @ Thu, 08 Oct 2020 22:01:46: 22000000 INFO @ Thu, 08 Oct 2020 22:01:48: 25000000 INFO @ Thu, 08 Oct 2020 22:01:48: 18000000 INFO @ Thu, 08 Oct 2020 22:01:53: 23000000 INFO @ Thu, 08 Oct 2020 22:01:56: 26000000 INFO @ Thu, 08 Oct 2020 22:01:56: 19000000 INFO @ Thu, 08 Oct 2020 22:02:01: 24000000 INFO @ Thu, 08 Oct 2020 22:02:03: 20000000 INFO @ Thu, 08 Oct 2020 22:02:03: 27000000 INFO @ Thu, 08 Oct 2020 22:02:08: 25000000 INFO @ Thu, 08 Oct 2020 22:02:11: 21000000 INFO @ Thu, 08 Oct 2020 22:02:11: 28000000 INFO @ Thu, 08 Oct 2020 22:02:15: 26000000 INFO @ Thu, 08 Oct 2020 22:02:18: 22000000 INFO @ Thu, 08 Oct 2020 22:02:19: 29000000 INFO @ Thu, 08 Oct 2020 22:02:23: 27000000 INFO @ Thu, 08 Oct 2020 22:02:25: 23000000 INFO @ Thu, 08 Oct 2020 22:02:27: 30000000 INFO @ Thu, 08 Oct 2020 22:02:30: 28000000 INFO @ Thu, 08 Oct 2020 22:02:33: 24000000 INFO @ Thu, 08 Oct 2020 22:02:35: 31000000 INFO @ Thu, 08 Oct 2020 22:02:38: 29000000 INFO @ Thu, 08 Oct 2020 22:02:41: 25000000 INFO @ Thu, 08 Oct 2020 22:02:43: 32000000 INFO @ Thu, 08 Oct 2020 22:02:46: 30000000 INFO @ Thu, 08 Oct 2020 22:02:48: 26000000 INFO @ Thu, 08 Oct 2020 22:02:51: 33000000 INFO @ Thu, 08 Oct 2020 22:02:54: 31000000 INFO @ Thu, 08 Oct 2020 22:02:56: 27000000 INFO @ Thu, 08 Oct 2020 22:02:58: 34000000 INFO @ Thu, 08 Oct 2020 22:03:01: 32000000 INFO @ Thu, 08 Oct 2020 22:03:04: 28000000 INFO @ Thu, 08 Oct 2020 22:03:06: 35000000 INFO @ Thu, 08 Oct 2020 22:03:09: 33000000 INFO @ Thu, 08 Oct 2020 22:03:12: 29000000 INFO @ Thu, 08 Oct 2020 22:03:14: 36000000 INFO @ Thu, 08 Oct 2020 22:03:16: 34000000 INFO @ Thu, 08 Oct 2020 22:03:19: 30000000 INFO @ Thu, 08 Oct 2020 22:03:22: 37000000 INFO @ Thu, 08 Oct 2020 22:03:24: 35000000 INFO @ Thu, 08 Oct 2020 22:03:26: 31000000 INFO @ Thu, 08 Oct 2020 22:03:30: 38000000 INFO @ Thu, 08 Oct 2020 22:03:31: 36000000 INFO @ Thu, 08 Oct 2020 22:03:34: 32000000 INFO @ Thu, 08 Oct 2020 22:03:38: 39000000 INFO @ Thu, 08 Oct 2020 22:03:39: 37000000 INFO @ Thu, 08 Oct 2020 22:03:41: 33000000 INFO @ Thu, 08 Oct 2020 22:03:46: 38000000 INFO @ Thu, 08 Oct 2020 22:03:46: 40000000 INFO @ Thu, 08 Oct 2020 22:03:48: 34000000 INFO @ Thu, 08 Oct 2020 22:03:53: 39000000 INFO @ Thu, 08 Oct 2020 22:03:54: 41000000 INFO @ Thu, 08 Oct 2020 22:03:56: 35000000 INFO @ Thu, 08 Oct 2020 22:04:01: 40000000 INFO @ Thu, 08 Oct 2020 22:04:02: 42000000 INFO @ Thu, 08 Oct 2020 22:04:03: 36000000 INFO @ Thu, 08 Oct 2020 22:04:08: 41000000 INFO @ Thu, 08 Oct 2020 22:04:09: 43000000 INFO @ Thu, 08 Oct 2020 22:04:11: 37000000 INFO @ Thu, 08 Oct 2020 22:04:16: 42000000 INFO @ Thu, 08 Oct 2020 22:04:17: 44000000 INFO @ Thu, 08 Oct 2020 22:04:18: 38000000 INFO @ Thu, 08 Oct 2020 22:04:23: 43000000 INFO @ Thu, 08 Oct 2020 22:04:25: 45000000 INFO @ Thu, 08 Oct 2020 22:04:26: 39000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 22:04:31: 44000000 INFO @ Thu, 08 Oct 2020 22:04:33: 46000000 INFO @ Thu, 08 Oct 2020 22:04:33: 40000000 INFO @ Thu, 08 Oct 2020 22:04:38: 45000000 INFO @ Thu, 08 Oct 2020 22:04:41: 41000000 INFO @ Thu, 08 Oct 2020 22:04:41: 47000000 INFO @ Thu, 08 Oct 2020 22:04:46: 46000000 INFO @ Thu, 08 Oct 2020 22:04:48: 42000000 INFO @ Thu, 08 Oct 2020 22:04:49: 48000000 INFO @ Thu, 08 Oct 2020 22:04:53: 47000000 INFO @ Thu, 08 Oct 2020 22:04:56: 43000000 INFO @ Thu, 08 Oct 2020 22:04:57: 49000000 INFO @ Thu, 08 Oct 2020 22:05:01: 48000000 INFO @ Thu, 08 Oct 2020 22:05:03: 44000000 INFO @ Thu, 08 Oct 2020 22:05:05: 50000000 INFO @ Thu, 08 Oct 2020 22:05:08: 49000000 INFO @ Thu, 08 Oct 2020 22:05:11: 45000000 INFO @ Thu, 08 Oct 2020 22:05:13: 51000000 INFO @ Thu, 08 Oct 2020 22:05:16: 50000000 INFO @ Thu, 08 Oct 2020 22:05:18: 46000000 INFO @ Thu, 08 Oct 2020 22:05:22: 52000000 INFO @ Thu, 08 Oct 2020 22:05:23: 51000000 INFO @ Thu, 08 Oct 2020 22:05:26: 47000000 INFO @ Thu, 08 Oct 2020 22:05:30: 53000000 INFO @ Thu, 08 Oct 2020 22:05:31: 52000000 INFO @ Thu, 08 Oct 2020 22:05:33: 48000000 INFO @ Thu, 08 Oct 2020 22:05:38: 54000000 INFO @ Thu, 08 Oct 2020 22:05:38: 53000000 INFO @ Thu, 08 Oct 2020 22:05:41: 49000000 INFO @ Thu, 08 Oct 2020 22:05:45: 54000000 INFO @ Thu, 08 Oct 2020 22:05:46: 55000000 INFO @ Thu, 08 Oct 2020 22:05:48: 50000000 INFO @ Thu, 08 Oct 2020 22:05:53: 55000000 INFO @ Thu, 08 Oct 2020 22:05:54: 56000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 22:05:56: 51000000 INFO @ Thu, 08 Oct 2020 22:06:01: 56000000 INFO @ Thu, 08 Oct 2020 22:06:02: 57000000 INFO @ Thu, 08 Oct 2020 22:06:04: 52000000 INFO @ Thu, 08 Oct 2020 22:06:08: 57000000 INFO @ Thu, 08 Oct 2020 22:06:11: 58000000 INFO @ Thu, 08 Oct 2020 22:06:12: 53000000 INFO @ Thu, 08 Oct 2020 22:06:16: 58000000 INFO @ Thu, 08 Oct 2020 22:06:19: 54000000 INFO @ Thu, 08 Oct 2020 22:06:19: 59000000 INFO @ Thu, 08 Oct 2020 22:06:24: 59000000 INFO @ Thu, 08 Oct 2020 22:06:27: 55000000 INFO @ Thu, 08 Oct 2020 22:06:27: 60000000 INFO @ Thu, 08 Oct 2020 22:06:31: 60000000 INFO @ Thu, 08 Oct 2020 22:06:34: 56000000 INFO @ Thu, 08 Oct 2020 22:06:36: 61000000 INFO @ Thu, 08 Oct 2020 22:06:39: 61000000 INFO @ Thu, 08 Oct 2020 22:06:42: 57000000 INFO @ Thu, 08 Oct 2020 22:06:44: 62000000 INFO @ Thu, 08 Oct 2020 22:06:47: 62000000 INFO @ Thu, 08 Oct 2020 22:06:50: 58000000 INFO @ Thu, 08 Oct 2020 22:06:53: 63000000 INFO @ Thu, 08 Oct 2020 22:06:55: 63000000 INFO @ Thu, 08 Oct 2020 22:06:58: 59000000 INFO @ Thu, 08 Oct 2020 22:07:01: 64000000 INFO @ Thu, 08 Oct 2020 22:07:03: 64000000 INFO @ Thu, 08 Oct 2020 22:07:06: 60000000 INFO @ Thu, 08 Oct 2020 22:07:09: 65000000 INFO @ Thu, 08 Oct 2020 22:07:11: 65000000 INFO @ Thu, 08 Oct 2020 22:07:14: 61000000 INFO @ Thu, 08 Oct 2020 22:07:18: 66000000 INFO @ Thu, 08 Oct 2020 22:07:18: 66000000 INFO @ Thu, 08 Oct 2020 22:07:22: 62000000 INFO @ Thu, 08 Oct 2020 22:07:26: 67000000 INFO @ Thu, 08 Oct 2020 22:07:27: 67000000 INFO @ Thu, 08 Oct 2020 22:07:30: 63000000 INFO @ Thu, 08 Oct 2020 22:07:35: 68000000 INFO @ Thu, 08 Oct 2020 22:07:35: 68000000 INFO @ Thu, 08 Oct 2020 22:07:37: 64000000 INFO @ Thu, 08 Oct 2020 22:07:44: 69000000 INFO @ Thu, 08 Oct 2020 22:07:44: 69000000 INFO @ Thu, 08 Oct 2020 22:07:45: 65000000 INFO @ Thu, 08 Oct 2020 22:07:53: 70000000 INFO @ Thu, 08 Oct 2020 22:07:53: 70000000 INFO @ Thu, 08 Oct 2020 22:07:53: 66000000 INFO @ Thu, 08 Oct 2020 22:08:01: 67000000 INFO @ Thu, 08 Oct 2020 22:08:02: 71000000 INFO @ Thu, 08 Oct 2020 22:08:02: 71000000 INFO @ Thu, 08 Oct 2020 22:08:08: 68000000 INFO @ Thu, 08 Oct 2020 22:08:11: 72000000 INFO @ Thu, 08 Oct 2020 22:08:11: 72000000 INFO @ Thu, 08 Oct 2020 22:08:15: 69000000 INFO @ Thu, 08 Oct 2020 22:08:20: 73000000 INFO @ Thu, 08 Oct 2020 22:08:20: 73000000 INFO @ Thu, 08 Oct 2020 22:08:23: 70000000 INFO @ Thu, 08 Oct 2020 22:08:25: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 22:08:25: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 22:08:25: #1 total tags in treatment: 33661296 INFO @ Thu, 08 Oct 2020 22:08:25: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 22:08:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 22:08:25: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 22:08:25: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 22:08:25: #1 total tags in treatment: 33661296 INFO @ Thu, 08 Oct 2020 22:08:25: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 22:08:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 22:08:26: #1 tags after filtering in treatment: 25423039 INFO @ Thu, 08 Oct 2020 22:08:26: #1 Redundant rate of treatment: 0.24 INFO @ Thu, 08 Oct 2020 22:08:26: #1 finished! INFO @ Thu, 08 Oct 2020 22:08:26: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 22:08:26: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 22:08:26: #1 tags after filtering in treatment: 25423039 INFO @ Thu, 08 Oct 2020 22:08:26: #1 Redundant rate of treatment: 0.24 INFO @ Thu, 08 Oct 2020 22:08:26: #1 finished! INFO @ Thu, 08 Oct 2020 22:08:26: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 22:08:26: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 22:08:28: #2 number of paired peaks: 6 WARNING @ Thu, 08 Oct 2020 22:08:28: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 22:08:28: Process for pairing-model is terminated! INFO @ Thu, 08 Oct 2020 22:08:28: #2 number of paired peaks: 6 WARNING @ Thu, 08 Oct 2020 22:08:28: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 22:08:28: Process for pairing-model is terminated! INFO @ Thu, 08 Oct 2020 22:08:30: 71000000 cut: /home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.10_*.xls’: No such file or directory pass1 - making usageList (0 chroms): 1 millis rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.10_peaks.narrowPeak’: No such file or directory needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 22:08:37: 72000000 INFO @ Thu, 08 Oct 2020 22:08:43: 73000000 INFO @ Thu, 08 Oct 2020 22:08:47: #1 tag size is determined as 75 bps INFO @ Thu, 08 Oct 2020 22:08:47: #1 tag size = 75 INFO @ Thu, 08 Oct 2020 22:08:47: #1 total tags in treatment: 33661296 INFO @ Thu, 08 Oct 2020 22:08:47: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 22:08:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 22:08:47: #1 tags after filtering in treatment: 25423039 INFO @ Thu, 08 Oct 2020 22:08:47: #1 Redundant rate of treatment: 0.24 INFO @ Thu, 08 Oct 2020 22:08:47: #1 finished! INFO @ Thu, 08 Oct 2020 22:08:47: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 22:08:47: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 22:08:49: #2 number of paired peaks: 6 WARNING @ Thu, 08 Oct 2020 22:08:49: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 22:08:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5681703/SRX5681703.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling