Job ID = 4178479 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 31,371,192 reads read : 31,371,192 reads written : 31,371,192 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:35 31371192 reads; of these: 31371192 (100.00%) were unpaired; of these: 705872 (2.25%) aligned 0 times 20062485 (63.95%) aligned exactly 1 time 10602835 (33.80%) aligned >1 times 97.75% overall alignment rate Time searching: 00:11:35 Overall time: 00:11:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 9386522 / 30665320 = 0.3061 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 05 Dec 2019 12:53:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 12:53:55: #1 read tag files... INFO @ Thu, 05 Dec 2019 12:53:55: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 12:54:04: 1000000 INFO @ Thu, 05 Dec 2019 12:54:13: 2000000 INFO @ Thu, 05 Dec 2019 12:54:22: 3000000 INFO @ Thu, 05 Dec 2019 12:54:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 12:54:25: #1 read tag files... INFO @ Thu, 05 Dec 2019 12:54:25: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 12:54:31: 4000000 INFO @ Thu, 05 Dec 2019 12:54:32: 1000000 INFO @ Thu, 05 Dec 2019 12:54:39: 2000000 INFO @ Thu, 05 Dec 2019 12:54:39: 5000000 INFO @ Thu, 05 Dec 2019 12:54:46: 3000000 INFO @ Thu, 05 Dec 2019 12:54:48: 6000000 INFO @ Thu, 05 Dec 2019 12:54:53: 4000000 BedGraph に変換中... INFO @ Thu, 05 Dec 2019 12:54:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 12:54:55: #1 read tag files... INFO @ Thu, 05 Dec 2019 12:54:55: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 12:54:56: 7000000 INFO @ Thu, 05 Dec 2019 12:54:58: 5000000 INFO @ Thu, 05 Dec 2019 12:55:03: 1000000 INFO @ Thu, 05 Dec 2019 12:55:04: 8000000 INFO @ Thu, 05 Dec 2019 12:55:05: 6000000 INFO @ Thu, 05 Dec 2019 12:55:10: 7000000 INFO @ Thu, 05 Dec 2019 12:55:12: 2000000 INFO @ Thu, 05 Dec 2019 12:55:12: 9000000 INFO @ Thu, 05 Dec 2019 12:55:16: 8000000 INFO @ Thu, 05 Dec 2019 12:55:20: 10000000 INFO @ Thu, 05 Dec 2019 12:55:22: 3000000 INFO @ Thu, 05 Dec 2019 12:55:22: 9000000 INFO @ Thu, 05 Dec 2019 12:55:28: 10000000 INFO @ Thu, 05 Dec 2019 12:55:29: 11000000 INFO @ Thu, 05 Dec 2019 12:55:31: 4000000 INFO @ Thu, 05 Dec 2019 12:55:33: 11000000 INFO @ Thu, 05 Dec 2019 12:55:38: 12000000 INFO @ Thu, 05 Dec 2019 12:55:39: 12000000 INFO @ Thu, 05 Dec 2019 12:55:39: 5000000 INFO @ Thu, 05 Dec 2019 12:55:46: 13000000 INFO @ Thu, 05 Dec 2019 12:55:47: 13000000 INFO @ Thu, 05 Dec 2019 12:55:48: 6000000 INFO @ Thu, 05 Dec 2019 12:55:52: 14000000 INFO @ Thu, 05 Dec 2019 12:55:55: 14000000 INFO @ Thu, 05 Dec 2019 12:55:57: 7000000 INFO @ Thu, 05 Dec 2019 12:55:58: 15000000 INFO @ Thu, 05 Dec 2019 12:56:03: 15000000 INFO @ Thu, 05 Dec 2019 12:56:04: 8000000 INFO @ Thu, 05 Dec 2019 12:56:05: 16000000 INFO @ Thu, 05 Dec 2019 12:56:11: 16000000 INFO @ Thu, 05 Dec 2019 12:56:11: 17000000 INFO @ Thu, 05 Dec 2019 12:56:12: 9000000 INFO @ Thu, 05 Dec 2019 12:56:18: 18000000 INFO @ Thu, 05 Dec 2019 12:56:19: 17000000 INFO @ Thu, 05 Dec 2019 12:56:21: 10000000 INFO @ Thu, 05 Dec 2019 12:56:24: 19000000 INFO @ Thu, 05 Dec 2019 12:56:28: 18000000 INFO @ Thu, 05 Dec 2019 12:56:31: 20000000 INFO @ Thu, 05 Dec 2019 12:56:31: 11000000 INFO @ Thu, 05 Dec 2019 12:56:36: 19000000 INFO @ Thu, 05 Dec 2019 12:56:37: 21000000 INFO @ Thu, 05 Dec 2019 12:56:39: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 12:56:39: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 12:56:39: #1 total tags in treatment: 21278798 INFO @ Thu, 05 Dec 2019 12:56:39: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 12:56:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 12:56:39: #1 tags after filtering in treatment: 21278798 INFO @ Thu, 05 Dec 2019 12:56:39: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 12:56:39: #1 finished! INFO @ Thu, 05 Dec 2019 12:56:39: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 12:56:39: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 12:56:40: 12000000 INFO @ Thu, 05 Dec 2019 12:56:41: #2 number of paired peaks: 9 WARNING @ Thu, 05 Dec 2019 12:56:41: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 12:56:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 12:56:45: 20000000 INFO @ Thu, 05 Dec 2019 12:56:49: 13000000 INFO @ Thu, 05 Dec 2019 12:56:54: 21000000 INFO @ Thu, 05 Dec 2019 12:56:56: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 12:56:56: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 12:56:56: #1 total tags in treatment: 21278798 INFO @ Thu, 05 Dec 2019 12:56:56: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 12:56:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 12:56:57: #1 tags after filtering in treatment: 21278798 INFO @ Thu, 05 Dec 2019 12:56:57: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 12:56:57: #1 finished! INFO @ Thu, 05 Dec 2019 12:56:57: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 12:56:57: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 12:56:58: 14000000 INFO @ Thu, 05 Dec 2019 12:56:58: #2 number of paired peaks: 9 WARNING @ Thu, 05 Dec 2019 12:56:58: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 12:56:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 12:57:07: 15000000 INFO @ Thu, 05 Dec 2019 12:57:16: 16000000 INFO @ Thu, 05 Dec 2019 12:57:25: 17000000 INFO @ Thu, 05 Dec 2019 12:57:34: 18000000 INFO @ Thu, 05 Dec 2019 12:57:42: 19000000 INFO @ Thu, 05 Dec 2019 12:57:51: 20000000 INFO @ Thu, 05 Dec 2019 12:57:59: 21000000 INFO @ Thu, 05 Dec 2019 12:58:02: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 12:58:02: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 12:58:02: #1 total tags in treatment: 21278798 INFO @ Thu, 05 Dec 2019 12:58:02: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 12:58:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 12:58:02: #1 tags after filtering in treatment: 21278798 INFO @ Thu, 05 Dec 2019 12:58:02: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 12:58:02: #1 finished! INFO @ Thu, 05 Dec 2019 12:58:02: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 12:58:02: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 12:58:03: #2 number of paired peaks: 9 WARNING @ Thu, 05 Dec 2019 12:58:03: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 12:58:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5535382/SRX5535382.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。