Job ID = 6528365 SRX = SRX5431041 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:48:43 prefetch.2.10.7: 1) Downloading 'SRR8632340'... 2020-06-29T14:48:43 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:50:24 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:50:24 prefetch.2.10.7: 1) 'SRR8632340' was downloaded successfully Read 20512187 spots for SRR8632340/SRR8632340.sra Written 20512187 spots for SRR8632340/SRR8632340.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:59 20512187 reads; of these: 20512187 (100.00%) were unpaired; of these: 866450 (4.22%) aligned 0 times 15322213 (74.70%) aligned exactly 1 time 4323524 (21.08%) aligned >1 times 95.78% overall alignment rate Time searching: 00:05:59 Overall time: 00:05:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1852061 / 19645737 = 0.0943 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:06:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:06:33: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:06:33: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:06:38: 1000000 INFO @ Tue, 30 Jun 2020 00:06:44: 2000000 INFO @ Tue, 30 Jun 2020 00:06:49: 3000000 INFO @ Tue, 30 Jun 2020 00:06:55: 4000000 INFO @ Tue, 30 Jun 2020 00:07:00: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:07:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:07:03: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:07:03: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:07:06: 6000000 INFO @ Tue, 30 Jun 2020 00:07:09: 1000000 INFO @ Tue, 30 Jun 2020 00:07:12: 7000000 INFO @ Tue, 30 Jun 2020 00:07:15: 2000000 INFO @ Tue, 30 Jun 2020 00:07:18: 8000000 INFO @ Tue, 30 Jun 2020 00:07:22: 3000000 INFO @ Tue, 30 Jun 2020 00:07:24: 9000000 INFO @ Tue, 30 Jun 2020 00:07:28: 4000000 INFO @ Tue, 30 Jun 2020 00:07:31: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:07:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:07:33: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:07:33: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:07:34: 5000000 INFO @ Tue, 30 Jun 2020 00:07:37: 11000000 INFO @ Tue, 30 Jun 2020 00:07:40: 1000000 INFO @ Tue, 30 Jun 2020 00:07:41: 6000000 INFO @ Tue, 30 Jun 2020 00:07:44: 12000000 INFO @ Tue, 30 Jun 2020 00:07:46: 2000000 INFO @ Tue, 30 Jun 2020 00:07:47: 7000000 INFO @ Tue, 30 Jun 2020 00:07:50: 13000000 INFO @ Tue, 30 Jun 2020 00:07:53: 3000000 INFO @ Tue, 30 Jun 2020 00:07:54: 8000000 INFO @ Tue, 30 Jun 2020 00:07:57: 14000000 INFO @ Tue, 30 Jun 2020 00:08:00: 4000000 INFO @ Tue, 30 Jun 2020 00:08:00: 9000000 INFO @ Tue, 30 Jun 2020 00:08:04: 15000000 INFO @ Tue, 30 Jun 2020 00:08:06: 5000000 INFO @ Tue, 30 Jun 2020 00:08:07: 10000000 INFO @ Tue, 30 Jun 2020 00:08:10: 16000000 INFO @ Tue, 30 Jun 2020 00:08:13: 6000000 INFO @ Tue, 30 Jun 2020 00:08:14: 11000000 INFO @ Tue, 30 Jun 2020 00:08:17: 17000000 INFO @ Tue, 30 Jun 2020 00:08:19: 7000000 INFO @ Tue, 30 Jun 2020 00:08:20: 12000000 INFO @ Tue, 30 Jun 2020 00:08:22: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:08:22: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:08:22: #1 total tags in treatment: 17793676 INFO @ Tue, 30 Jun 2020 00:08:22: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:08:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:08:22: #1 tags after filtering in treatment: 17793676 INFO @ Tue, 30 Jun 2020 00:08:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:08:22: #1 finished! INFO @ Tue, 30 Jun 2020 00:08:22: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:08:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:08:23: #2 number of paired peaks: 80 WARNING @ Tue, 30 Jun 2020 00:08:23: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:08:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:08:26: 8000000 INFO @ Tue, 30 Jun 2020 00:08:27: 13000000 INFO @ Tue, 30 Jun 2020 00:08:32: 9000000 INFO @ Tue, 30 Jun 2020 00:08:33: 14000000 INFO @ Tue, 30 Jun 2020 00:08:39: 10000000 INFO @ Tue, 30 Jun 2020 00:08:40: 15000000 INFO @ Tue, 30 Jun 2020 00:08:45: 11000000 INFO @ Tue, 30 Jun 2020 00:08:46: 16000000 INFO @ Tue, 30 Jun 2020 00:08:52: 12000000 INFO @ Tue, 30 Jun 2020 00:08:53: 17000000 INFO @ Tue, 30 Jun 2020 00:08:58: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:08:58: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:08:58: #1 total tags in treatment: 17793676 INFO @ Tue, 30 Jun 2020 00:08:58: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:08:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:08:58: #1 tags after filtering in treatment: 17793676 INFO @ Tue, 30 Jun 2020 00:08:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:08:58: #1 finished! INFO @ Tue, 30 Jun 2020 00:08:58: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:08:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:08:59: 13000000 INFO @ Tue, 30 Jun 2020 00:08:59: #2 number of paired peaks: 80 WARNING @ Tue, 30 Jun 2020 00:08:59: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:08:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:09:05: 14000000 INFO @ Tue, 30 Jun 2020 00:09:11: 15000000 INFO @ Tue, 30 Jun 2020 00:09:16: 16000000 INFO @ Tue, 30 Jun 2020 00:09:22: 17000000 INFO @ Tue, 30 Jun 2020 00:09:26: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:09:26: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:09:26: #1 total tags in treatment: 17793676 INFO @ Tue, 30 Jun 2020 00:09:26: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:09:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:09:26: #1 tags after filtering in treatment: 17793676 INFO @ Tue, 30 Jun 2020 00:09:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:09:26: #1 finished! INFO @ Tue, 30 Jun 2020 00:09:26: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:09:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:09:28: #2 number of paired peaks: 80 WARNING @ Tue, 30 Jun 2020 00:09:28: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:09:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431041/SRX5431041.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。