Job ID = 6528363 SRX = SRX5431038 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:52:54 prefetch.2.10.7: 1) Downloading 'SRR8632337'... 2020-06-29T14:52:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:53:57 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:53:58 prefetch.2.10.7: 'SRR8632337' is valid 2020-06-29T14:53:58 prefetch.2.10.7: 1) 'SRR8632337' was downloaded successfully Read 15111122 spots for SRR8632337/SRR8632337.sra Written 15111122 spots for SRR8632337/SRR8632337.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:38 15111122 reads; of these: 15111122 (100.00%) were unpaired; of these: 1025161 (6.78%) aligned 0 times 10683872 (70.70%) aligned exactly 1 time 3402089 (22.51%) aligned >1 times 93.22% overall alignment rate Time searching: 00:04:38 Overall time: 00:04:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 962416 / 14085961 = 0.0683 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:06:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:06:28: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:06:28: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:06:34: 1000000 INFO @ Tue, 30 Jun 2020 00:06:39: 2000000 INFO @ Tue, 30 Jun 2020 00:06:45: 3000000 INFO @ Tue, 30 Jun 2020 00:06:51: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:06:57: 5000000 INFO @ Tue, 30 Jun 2020 00:06:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:06:58: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:06:58: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:07:03: 6000000 INFO @ Tue, 30 Jun 2020 00:07:06: 1000000 INFO @ Tue, 30 Jun 2020 00:07:10: 7000000 INFO @ Tue, 30 Jun 2020 00:07:14: 2000000 INFO @ Tue, 30 Jun 2020 00:07:17: 8000000 INFO @ Tue, 30 Jun 2020 00:07:21: 3000000 INFO @ Tue, 30 Jun 2020 00:07:23: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:07:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:07:28: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:07:28: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:07:29: 4000000 INFO @ Tue, 30 Jun 2020 00:07:30: 10000000 INFO @ Tue, 30 Jun 2020 00:07:35: 1000000 INFO @ Tue, 30 Jun 2020 00:07:37: 5000000 INFO @ Tue, 30 Jun 2020 00:07:37: 11000000 INFO @ Tue, 30 Jun 2020 00:07:42: 2000000 INFO @ Tue, 30 Jun 2020 00:07:45: 6000000 INFO @ Tue, 30 Jun 2020 00:07:45: 12000000 INFO @ Tue, 30 Jun 2020 00:07:49: 3000000 INFO @ Tue, 30 Jun 2020 00:07:52: 13000000 INFO @ Tue, 30 Jun 2020 00:07:52: 7000000 INFO @ Tue, 30 Jun 2020 00:07:53: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:07:53: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:07:53: #1 total tags in treatment: 13123545 INFO @ Tue, 30 Jun 2020 00:07:53: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:07:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:07:53: #1 tags after filtering in treatment: 13123545 INFO @ Tue, 30 Jun 2020 00:07:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:07:53: #1 finished! INFO @ Tue, 30 Jun 2020 00:07:53: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:07:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:07:54: #2 number of paired peaks: 68 WARNING @ Tue, 30 Jun 2020 00:07:54: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:07:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:07:56: 4000000 INFO @ Tue, 30 Jun 2020 00:07:59: 8000000 INFO @ Tue, 30 Jun 2020 00:08:03: 5000000 INFO @ Tue, 30 Jun 2020 00:08:07: 9000000 INFO @ Tue, 30 Jun 2020 00:08:10: 6000000 INFO @ Tue, 30 Jun 2020 00:08:14: 10000000 INFO @ Tue, 30 Jun 2020 00:08:17: 7000000 INFO @ Tue, 30 Jun 2020 00:08:22: 11000000 INFO @ Tue, 30 Jun 2020 00:08:24: 8000000 INFO @ Tue, 30 Jun 2020 00:08:29: 12000000 INFO @ Tue, 30 Jun 2020 00:08:30: 9000000 INFO @ Tue, 30 Jun 2020 00:08:36: 13000000 INFO @ Tue, 30 Jun 2020 00:08:37: 10000000 INFO @ Tue, 30 Jun 2020 00:08:37: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:08:37: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:08:37: #1 total tags in treatment: 13123545 INFO @ Tue, 30 Jun 2020 00:08:37: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:08:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:08:37: #1 tags after filtering in treatment: 13123545 INFO @ Tue, 30 Jun 2020 00:08:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:08:37: #1 finished! INFO @ Tue, 30 Jun 2020 00:08:37: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:08:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:08:38: #2 number of paired peaks: 68 WARNING @ Tue, 30 Jun 2020 00:08:38: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:08:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:08:44: 11000000 INFO @ Tue, 30 Jun 2020 00:08:50: 12000000 INFO @ Tue, 30 Jun 2020 00:08:56: 13000000 INFO @ Tue, 30 Jun 2020 00:08:56: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:08:56: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:08:56: #1 total tags in treatment: 13123545 INFO @ Tue, 30 Jun 2020 00:08:56: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:08:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:08:56: #1 tags after filtering in treatment: 13123545 INFO @ Tue, 30 Jun 2020 00:08:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:08:56: #1 finished! INFO @ Tue, 30 Jun 2020 00:08:56: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:08:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:08:57: #2 number of paired peaks: 68 WARNING @ Tue, 30 Jun 2020 00:08:57: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:08:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5431038/SRX5431038.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。