Job ID = 2590922 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 387 reads read : 387 reads written : 387 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR8559078.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:00 387 reads; of these: 387 (100.00%) were unpaired; of these: 161 (41.60%) aligned 0 times 221 (57.11%) aligned exactly 1 time 5 (1.29%) aligned >1 times 58.40% overall alignment rate Time searching: 00:00:00 Overall time: 00:00:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 3 / 226 = 0.0133 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Mon, 12 Aug 2019 23:54:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:54:35: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:54:35: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:54:35: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:54:35: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:54:35: #1 total tags in treatment: 223 INFO @ Mon, 12 Aug 2019 23:54:35: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:54:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:54:35: #1 tags after filtering in treatment: 223 INFO @ Mon, 12 Aug 2019 23:54:35: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:54:35: #1 finished! INFO @ Mon, 12 Aug 2019 23:54:35: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:54:35: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:54:35: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 23:54:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:54:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:54:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:54:36: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:54:36: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:54:36: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:54:36: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:54:36: #1 total tags in treatment: 223 INFO @ Mon, 12 Aug 2019 23:54:36: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:54:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:54:36: #1 tags after filtering in treatment: 223 INFO @ Mon, 12 Aug 2019 23:54:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:54:36: #1 finished! INFO @ Mon, 12 Aug 2019 23:54:36: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:54:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:54:36: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 23:54:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:54:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:54:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:54:37: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:54:37: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:54:37: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:54:37: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:54:37: #1 total tags in treatment: 223 INFO @ Mon, 12 Aug 2019 23:54:37: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:54:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:54:37: #1 tags after filtering in treatment: 223 INFO @ Mon, 12 Aug 2019 23:54:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:54:37: #1 finished! INFO @ Mon, 12 Aug 2019 23:54:37: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:54:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:54:37: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 23:54:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:54:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5360602/SRX5360602.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling