Job ID = 1307862 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 34,725,899 reads read : 69,451,798 reads written : 34,725,899 reads 0-length : 34,725,899 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:24 34725899 reads; of these: 34725899 (100.00%) were unpaired; of these: 1276327 (3.68%) aligned 0 times 22990416 (66.21%) aligned exactly 1 time 10459156 (30.12%) aligned >1 times 96.32% overall alignment rate Time searching: 00:19:24 Overall time: 00:19:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4349354 / 33449572 = 0.1300 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 23:17:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 23:17:18: #1 read tag files... INFO @ Mon, 03 Jun 2019 23:17:18: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 23:17:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 23:17:18: #1 read tag files... INFO @ Mon, 03 Jun 2019 23:17:18: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 23:17:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 23:17:18: #1 read tag files... INFO @ Mon, 03 Jun 2019 23:17:18: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 23:17:27: 1000000 INFO @ Mon, 03 Jun 2019 23:17:29: 1000000 INFO @ Mon, 03 Jun 2019 23:17:31: 1000000 INFO @ Mon, 03 Jun 2019 23:17:36: 2000000 INFO @ Mon, 03 Jun 2019 23:17:39: 2000000 INFO @ Mon, 03 Jun 2019 23:17:42: 2000000 INFO @ Mon, 03 Jun 2019 23:17:44: 3000000 INFO @ Mon, 03 Jun 2019 23:17:49: 3000000 INFO @ Mon, 03 Jun 2019 23:17:52: 4000000 INFO @ Mon, 03 Jun 2019 23:17:54: 3000000 INFO @ Mon, 03 Jun 2019 23:18:00: 4000000 INFO @ Mon, 03 Jun 2019 23:18:01: 5000000 INFO @ Mon, 03 Jun 2019 23:18:05: 4000000 INFO @ Mon, 03 Jun 2019 23:18:09: 6000000 INFO @ Mon, 03 Jun 2019 23:18:10: 5000000 INFO @ Mon, 03 Jun 2019 23:18:16: 5000000 INFO @ Mon, 03 Jun 2019 23:18:18: 7000000 INFO @ Mon, 03 Jun 2019 23:18:21: 6000000 INFO @ Mon, 03 Jun 2019 23:18:26: 8000000 INFO @ Mon, 03 Jun 2019 23:18:28: 6000000 INFO @ Mon, 03 Jun 2019 23:18:31: 7000000 INFO @ Mon, 03 Jun 2019 23:18:35: 9000000 INFO @ Mon, 03 Jun 2019 23:18:39: 8000000 INFO @ Mon, 03 Jun 2019 23:18:40: 7000000 INFO @ Mon, 03 Jun 2019 23:18:44: 10000000 INFO @ Mon, 03 Jun 2019 23:18:47: 9000000 INFO @ Mon, 03 Jun 2019 23:18:51: 8000000 INFO @ Mon, 03 Jun 2019 23:18:52: 11000000 INFO @ Mon, 03 Jun 2019 23:18:55: 10000000 INFO @ Mon, 03 Jun 2019 23:19:01: 12000000 INFO @ Mon, 03 Jun 2019 23:19:02: 9000000 INFO @ Mon, 03 Jun 2019 23:19:03: 11000000 INFO @ Mon, 03 Jun 2019 23:19:09: 13000000 INFO @ Mon, 03 Jun 2019 23:19:11: 12000000 INFO @ Mon, 03 Jun 2019 23:19:13: 10000000 INFO @ Mon, 03 Jun 2019 23:19:17: 14000000 INFO @ Mon, 03 Jun 2019 23:19:19: 13000000 INFO @ Mon, 03 Jun 2019 23:19:25: 11000000 INFO @ Mon, 03 Jun 2019 23:19:26: 15000000 INFO @ Mon, 03 Jun 2019 23:19:27: 14000000 INFO @ Mon, 03 Jun 2019 23:19:34: 16000000 INFO @ Mon, 03 Jun 2019 23:19:35: 15000000 INFO @ Mon, 03 Jun 2019 23:19:37: 12000000 INFO @ Mon, 03 Jun 2019 23:19:42: 17000000 INFO @ Mon, 03 Jun 2019 23:19:43: 16000000 INFO @ Mon, 03 Jun 2019 23:19:48: 13000000 INFO @ Mon, 03 Jun 2019 23:19:51: 17000000 INFO @ Mon, 03 Jun 2019 23:19:51: 18000000 INFO @ Mon, 03 Jun 2019 23:19:59: 18000000 INFO @ Mon, 03 Jun 2019 23:19:59: 14000000 INFO @ Mon, 03 Jun 2019 23:19:59: 19000000 INFO @ Mon, 03 Jun 2019 23:20:07: 19000000 INFO @ Mon, 03 Jun 2019 23:20:08: 20000000 INFO @ Mon, 03 Jun 2019 23:20:10: 15000000 INFO @ Mon, 03 Jun 2019 23:20:15: 20000000 INFO @ Mon, 03 Jun 2019 23:20:16: 21000000 INFO @ Mon, 03 Jun 2019 23:20:21: 16000000 INFO @ Mon, 03 Jun 2019 23:20:23: 21000000 INFO @ Mon, 03 Jun 2019 23:20:24: 22000000 INFO @ Mon, 03 Jun 2019 23:20:30: 22000000 INFO @ Mon, 03 Jun 2019 23:20:33: 17000000 INFO @ Mon, 03 Jun 2019 23:20:33: 23000000 INFO @ Mon, 03 Jun 2019 23:20:38: 23000000 INFO @ Mon, 03 Jun 2019 23:20:41: 24000000 INFO @ Mon, 03 Jun 2019 23:20:44: 18000000 INFO @ Mon, 03 Jun 2019 23:20:46: 24000000 INFO @ Mon, 03 Jun 2019 23:20:50: 25000000 INFO @ Mon, 03 Jun 2019 23:20:54: 25000000 INFO @ Mon, 03 Jun 2019 23:20:55: 19000000 INFO @ Mon, 03 Jun 2019 23:20:58: 26000000 INFO @ Mon, 03 Jun 2019 23:21:02: 26000000 INFO @ Mon, 03 Jun 2019 23:21:07: 27000000 INFO @ Mon, 03 Jun 2019 23:21:07: 20000000 INFO @ Mon, 03 Jun 2019 23:21:10: 27000000 INFO @ Mon, 03 Jun 2019 23:21:15: 28000000 INFO @ Mon, 03 Jun 2019 23:21:18: 21000000 INFO @ Mon, 03 Jun 2019 23:21:18: 28000000 INFO @ Mon, 03 Jun 2019 23:21:23: 29000000 INFO @ Mon, 03 Jun 2019 23:21:24: #1 tag size is determined as 65 bps INFO @ Mon, 03 Jun 2019 23:21:24: #1 tag size = 65 INFO @ Mon, 03 Jun 2019 23:21:24: #1 total tags in treatment: 29100218 INFO @ Mon, 03 Jun 2019 23:21:24: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 23:21:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 23:21:25: #1 tags after filtering in treatment: 29100218 INFO @ Mon, 03 Jun 2019 23:21:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 23:21:25: #1 finished! INFO @ Mon, 03 Jun 2019 23:21:25: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 23:21:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 23:21:26: 29000000 INFO @ Mon, 03 Jun 2019 23:21:27: #1 tag size is determined as 65 bps INFO @ Mon, 03 Jun 2019 23:21:27: #1 tag size = 65 INFO @ Mon, 03 Jun 2019 23:21:27: #1 total tags in treatment: 29100218 INFO @ Mon, 03 Jun 2019 23:21:27: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 23:21:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 23:21:27: #2 number of paired peaks: 50 WARNING @ Mon, 03 Jun 2019 23:21:27: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 23:21:27: Process for pairing-model is terminated! INFO @ Mon, 03 Jun 2019 23:21:27: #1 tags after filtering in treatment: 29100218 INFO @ Mon, 03 Jun 2019 23:21:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 23:21:27: #1 finished! INFO @ Mon, 03 Jun 2019 23:21:27: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 23:21:27: #2 looking for paired plus/minus strand peaks... cut: /home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 23:21:29: 22000000 INFO @ Mon, 03 Jun 2019 23:21:30: #2 number of paired peaks: 50 WARNING @ Mon, 03 Jun 2019 23:21:30: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 23:21:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 23:21:40: 23000000 INFO @ Mon, 03 Jun 2019 23:21:51: 24000000 INFO @ Mon, 03 Jun 2019 23:22:02: 25000000 INFO @ Mon, 03 Jun 2019 23:22:13: 26000000 INFO @ Mon, 03 Jun 2019 23:22:24: 27000000 INFO @ Mon, 03 Jun 2019 23:22:35: 28000000 INFO @ Mon, 03 Jun 2019 23:22:46: 29000000 INFO @ Mon, 03 Jun 2019 23:22:47: #1 tag size is determined as 65 bps INFO @ Mon, 03 Jun 2019 23:22:47: #1 tag size = 65 INFO @ Mon, 03 Jun 2019 23:22:47: #1 total tags in treatment: 29100218 INFO @ Mon, 03 Jun 2019 23:22:47: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 23:22:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 23:22:48: #1 tags after filtering in treatment: 29100218 INFO @ Mon, 03 Jun 2019 23:22:48: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 23:22:48: #1 finished! INFO @ Mon, 03 Jun 2019 23:22:48: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 23:22:48: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 23:22:50: #2 number of paired peaks: 50 WARNING @ Mon, 03 Jun 2019 23:22:50: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 23:22:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343120/SRX5343120.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。