Job ID = 1307680 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 32,207,368 reads read : 64,414,736 reads written : 32,207,368 reads 0-length : 32,207,368 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:24 32207368 reads; of these: 32207368 (100.00%) were unpaired; of these: 992837 (3.08%) aligned 0 times 21559916 (66.94%) aligned exactly 1 time 9654615 (29.98%) aligned >1 times 96.92% overall alignment rate Time searching: 00:17:24 Overall time: 00:17:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3901557 / 31214531 = 0.1250 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 23:06:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 23:06:42: #1 read tag files... INFO @ Mon, 03 Jun 2019 23:06:42: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 23:06:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 23:06:42: #1 read tag files... INFO @ Mon, 03 Jun 2019 23:06:42: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 23:06:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 23:06:42: #1 read tag files... INFO @ Mon, 03 Jun 2019 23:06:42: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 23:06:50: 1000000 INFO @ Mon, 03 Jun 2019 23:06:51: 1000000 INFO @ Mon, 03 Jun 2019 23:06:51: 1000000 INFO @ Mon, 03 Jun 2019 23:06:57: 2000000 INFO @ Mon, 03 Jun 2019 23:07:00: 2000000 INFO @ Mon, 03 Jun 2019 23:07:00: 2000000 INFO @ Mon, 03 Jun 2019 23:07:05: 3000000 INFO @ Mon, 03 Jun 2019 23:07:09: 3000000 INFO @ Mon, 03 Jun 2019 23:07:09: 3000000 INFO @ Mon, 03 Jun 2019 23:07:12: 4000000 INFO @ Mon, 03 Jun 2019 23:07:18: 4000000 INFO @ Mon, 03 Jun 2019 23:07:19: 4000000 INFO @ Mon, 03 Jun 2019 23:07:20: 5000000 INFO @ Mon, 03 Jun 2019 23:07:27: 5000000 INFO @ Mon, 03 Jun 2019 23:07:28: 5000000 INFO @ Mon, 03 Jun 2019 23:07:28: 6000000 INFO @ Mon, 03 Jun 2019 23:07:36: 7000000 INFO @ Mon, 03 Jun 2019 23:07:37: 6000000 INFO @ Mon, 03 Jun 2019 23:07:37: 6000000 INFO @ Mon, 03 Jun 2019 23:07:43: 8000000 INFO @ Mon, 03 Jun 2019 23:07:46: 7000000 INFO @ Mon, 03 Jun 2019 23:07:46: 7000000 INFO @ Mon, 03 Jun 2019 23:07:50: 9000000 INFO @ Mon, 03 Jun 2019 23:07:55: 8000000 INFO @ Mon, 03 Jun 2019 23:07:55: 8000000 INFO @ Mon, 03 Jun 2019 23:07:58: 10000000 INFO @ Mon, 03 Jun 2019 23:08:04: 9000000 INFO @ Mon, 03 Jun 2019 23:08:04: 9000000 INFO @ Mon, 03 Jun 2019 23:08:05: 11000000 INFO @ Mon, 03 Jun 2019 23:08:13: 12000000 INFO @ Mon, 03 Jun 2019 23:08:13: 10000000 INFO @ Mon, 03 Jun 2019 23:08:14: 10000000 INFO @ Mon, 03 Jun 2019 23:08:20: 13000000 INFO @ Mon, 03 Jun 2019 23:08:23: 11000000 INFO @ Mon, 03 Jun 2019 23:08:23: 11000000 INFO @ Mon, 03 Jun 2019 23:08:27: 14000000 INFO @ Mon, 03 Jun 2019 23:08:32: 12000000 INFO @ Mon, 03 Jun 2019 23:08:32: 12000000 INFO @ Mon, 03 Jun 2019 23:08:35: 15000000 INFO @ Mon, 03 Jun 2019 23:08:41: 13000000 INFO @ Mon, 03 Jun 2019 23:08:41: 13000000 INFO @ Mon, 03 Jun 2019 23:08:42: 16000000 INFO @ Mon, 03 Jun 2019 23:08:50: 17000000 INFO @ Mon, 03 Jun 2019 23:08:50: 14000000 INFO @ Mon, 03 Jun 2019 23:08:50: 14000000 INFO @ Mon, 03 Jun 2019 23:08:58: 18000000 INFO @ Mon, 03 Jun 2019 23:08:59: 15000000 INFO @ Mon, 03 Jun 2019 23:08:59: 15000000 INFO @ Mon, 03 Jun 2019 23:09:07: 19000000 INFO @ Mon, 03 Jun 2019 23:09:08: 16000000 INFO @ Mon, 03 Jun 2019 23:09:08: 16000000 INFO @ Mon, 03 Jun 2019 23:09:14: 20000000 INFO @ Mon, 03 Jun 2019 23:09:17: 17000000 INFO @ Mon, 03 Jun 2019 23:09:18: 17000000 INFO @ Mon, 03 Jun 2019 23:09:22: 21000000 INFO @ Mon, 03 Jun 2019 23:09:27: 18000000 INFO @ Mon, 03 Jun 2019 23:09:27: 18000000 INFO @ Mon, 03 Jun 2019 23:09:29: 22000000 INFO @ Mon, 03 Jun 2019 23:09:36: 19000000 INFO @ Mon, 03 Jun 2019 23:09:36: 19000000 INFO @ Mon, 03 Jun 2019 23:09:37: 23000000 INFO @ Mon, 03 Jun 2019 23:09:44: 24000000 INFO @ Mon, 03 Jun 2019 23:09:45: 20000000 INFO @ Mon, 03 Jun 2019 23:09:45: 20000000 INFO @ Mon, 03 Jun 2019 23:09:52: 25000000 INFO @ Mon, 03 Jun 2019 23:09:54: 21000000 INFO @ Mon, 03 Jun 2019 23:09:54: 21000000 INFO @ Mon, 03 Jun 2019 23:09:59: 26000000 INFO @ Mon, 03 Jun 2019 23:10:03: 22000000 INFO @ Mon, 03 Jun 2019 23:10:03: 22000000 INFO @ Mon, 03 Jun 2019 23:10:06: 27000000 INFO @ Mon, 03 Jun 2019 23:10:09: #1 tag size is determined as 65 bps INFO @ Mon, 03 Jun 2019 23:10:09: #1 tag size = 65 INFO @ Mon, 03 Jun 2019 23:10:09: #1 total tags in treatment: 27312974 INFO @ Mon, 03 Jun 2019 23:10:09: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 23:10:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 23:10:10: #1 tags after filtering in treatment: 27312974 INFO @ Mon, 03 Jun 2019 23:10:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 23:10:10: #1 finished! INFO @ Mon, 03 Jun 2019 23:10:10: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 23:10:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 23:10:12: #2 number of paired peaks: 62 WARNING @ Mon, 03 Jun 2019 23:10:12: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 23:10:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 23:10:12: 23000000 INFO @ Mon, 03 Jun 2019 23:10:12: 23000000 INFO @ Mon, 03 Jun 2019 23:10:21: 24000000 INFO @ Mon, 03 Jun 2019 23:10:21: 24000000 INFO @ Mon, 03 Jun 2019 23:10:30: 25000000 INFO @ Mon, 03 Jun 2019 23:10:30: 25000000 INFO @ Mon, 03 Jun 2019 23:10:38: 26000000 INFO @ Mon, 03 Jun 2019 23:10:38: 26000000 INFO @ Mon, 03 Jun 2019 23:10:47: 27000000 INFO @ Mon, 03 Jun 2019 23:10:47: 27000000 INFO @ Mon, 03 Jun 2019 23:10:50: #1 tag size is determined as 65 bps INFO @ Mon, 03 Jun 2019 23:10:50: #1 tag size is determined as 65 bps INFO @ Mon, 03 Jun 2019 23:10:50: #1 tag size = 65 INFO @ Mon, 03 Jun 2019 23:10:50: #1 total tags in treatment: 27312974 INFO @ Mon, 03 Jun 2019 23:10:50: #1 tag size = 65 INFO @ Mon, 03 Jun 2019 23:10:50: #1 total tags in treatment: 27312974 INFO @ Mon, 03 Jun 2019 23:10:50: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 23:10:50: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 23:10:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 23:10:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 23:10:50: #1 tags after filtering in treatment: 27312974 INFO @ Mon, 03 Jun 2019 23:10:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 23:10:50: #1 finished! INFO @ Mon, 03 Jun 2019 23:10:50: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 23:10:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 23:10:50: #1 tags after filtering in treatment: 27312974 INFO @ Mon, 03 Jun 2019 23:10:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 23:10:50: #1 finished! INFO @ Mon, 03 Jun 2019 23:10:50: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 23:10:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 23:10:53: #2 number of paired peaks: 62 WARNING @ Mon, 03 Jun 2019 23:10:53: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 23:10:53: Process for pairing-model is terminated! INFO @ Mon, 03 Jun 2019 23:10:53: #2 number of paired peaks: 62 WARNING @ Mon, 03 Jun 2019 23:10:53: Too few paired peaks (62) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 23:10:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5343104/SRX5343104.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。