Job ID = 6528298 SRX = SRX5287831 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:39:25 prefetch.2.10.7: 1) Downloading 'SRR8483062'... 2020-06-29T14:39:25 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:41:26 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:41:26 prefetch.2.10.7: 1) 'SRR8483062' was downloaded successfully 2020-06-29T14:41:26 prefetch.2.10.7: 'SRR8483062' has 0 unresolved dependencies Read 23430975 spots for SRR8483062/SRR8483062.sra Written 23430975 spots for SRR8483062/SRR8483062.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:37 23430975 reads; of these: 23430975 (100.00%) were unpaired; of these: 568575 (2.43%) aligned 0 times 17214880 (73.47%) aligned exactly 1 time 5647520 (24.10%) aligned >1 times 97.57% overall alignment rate Time searching: 00:10:37 Overall time: 00:10:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2231546 / 22862400 = 0.0976 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:09:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:09:14: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:09:14: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:09:20: 1000000 INFO @ Tue, 30 Jun 2020 00:09:26: 2000000 INFO @ Tue, 30 Jun 2020 00:09:32: 3000000 INFO @ Tue, 30 Jun 2020 00:09:38: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:09:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:09:44: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:09:44: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:09:45: 5000000 INFO @ Tue, 30 Jun 2020 00:09:52: 6000000 INFO @ Tue, 30 Jun 2020 00:09:52: 1000000 INFO @ Tue, 30 Jun 2020 00:09:59: 7000000 INFO @ Tue, 30 Jun 2020 00:10:00: 2000000 INFO @ Tue, 30 Jun 2020 00:10:06: 8000000 INFO @ Tue, 30 Jun 2020 00:10:08: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:10:13: 9000000 INFO @ Tue, 30 Jun 2020 00:10:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:10:14: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:10:14: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:10:15: 4000000 INFO @ Tue, 30 Jun 2020 00:10:21: 10000000 INFO @ Tue, 30 Jun 2020 00:10:22: 1000000 INFO @ Tue, 30 Jun 2020 00:10:23: 5000000 INFO @ Tue, 30 Jun 2020 00:10:28: 11000000 INFO @ Tue, 30 Jun 2020 00:10:29: 2000000 INFO @ Tue, 30 Jun 2020 00:10:31: 6000000 INFO @ Tue, 30 Jun 2020 00:10:35: 12000000 INFO @ Tue, 30 Jun 2020 00:10:36: 3000000 INFO @ Tue, 30 Jun 2020 00:10:39: 7000000 INFO @ Tue, 30 Jun 2020 00:10:43: 13000000 INFO @ Tue, 30 Jun 2020 00:10:44: 4000000 INFO @ Tue, 30 Jun 2020 00:10:47: 8000000 INFO @ Tue, 30 Jun 2020 00:10:50: 14000000 INFO @ Tue, 30 Jun 2020 00:10:51: 5000000 INFO @ Tue, 30 Jun 2020 00:10:55: 9000000 INFO @ Tue, 30 Jun 2020 00:10:57: 15000000 INFO @ Tue, 30 Jun 2020 00:10:59: 6000000 INFO @ Tue, 30 Jun 2020 00:11:03: 10000000 INFO @ Tue, 30 Jun 2020 00:11:05: 16000000 INFO @ Tue, 30 Jun 2020 00:11:06: 7000000 INFO @ Tue, 30 Jun 2020 00:11:11: 11000000 INFO @ Tue, 30 Jun 2020 00:11:12: 17000000 INFO @ Tue, 30 Jun 2020 00:11:14: 8000000 INFO @ Tue, 30 Jun 2020 00:11:18: 12000000 INFO @ Tue, 30 Jun 2020 00:11:20: 18000000 INFO @ Tue, 30 Jun 2020 00:11:21: 9000000 INFO @ Tue, 30 Jun 2020 00:11:26: 13000000 INFO @ Tue, 30 Jun 2020 00:11:27: 19000000 INFO @ Tue, 30 Jun 2020 00:11:28: 10000000 INFO @ Tue, 30 Jun 2020 00:11:34: 14000000 INFO @ Tue, 30 Jun 2020 00:11:35: 20000000 INFO @ Tue, 30 Jun 2020 00:11:35: 11000000 INFO @ Tue, 30 Jun 2020 00:11:39: #1 tag size is determined as 74 bps INFO @ Tue, 30 Jun 2020 00:11:39: #1 tag size = 74 INFO @ Tue, 30 Jun 2020 00:11:39: #1 total tags in treatment: 20630854 INFO @ Tue, 30 Jun 2020 00:11:39: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:11:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:11:40: #1 tags after filtering in treatment: 20630854 INFO @ Tue, 30 Jun 2020 00:11:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:11:40: #1 finished! INFO @ Tue, 30 Jun 2020 00:11:40: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:11:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:11:41: #2 number of paired peaks: 51 WARNING @ Tue, 30 Jun 2020 00:11:41: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:11:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:11:42: 15000000 INFO @ Tue, 30 Jun 2020 00:11:43: 12000000 INFO @ Tue, 30 Jun 2020 00:11:50: 16000000 INFO @ Tue, 30 Jun 2020 00:11:50: 13000000 INFO @ Tue, 30 Jun 2020 00:11:57: 14000000 INFO @ Tue, 30 Jun 2020 00:11:58: 17000000 INFO @ Tue, 30 Jun 2020 00:12:04: 15000000 INFO @ Tue, 30 Jun 2020 00:12:05: 18000000 INFO @ Tue, 30 Jun 2020 00:12:12: 16000000 INFO @ Tue, 30 Jun 2020 00:12:13: 19000000 INFO @ Tue, 30 Jun 2020 00:12:19: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:12:21: 20000000 INFO @ Tue, 30 Jun 2020 00:12:26: #1 tag size is determined as 74 bps INFO @ Tue, 30 Jun 2020 00:12:26: #1 tag size = 74 INFO @ Tue, 30 Jun 2020 00:12:26: #1 total tags in treatment: 20630854 INFO @ Tue, 30 Jun 2020 00:12:26: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:12:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:12:26: #1 tags after filtering in treatment: 20630854 INFO @ Tue, 30 Jun 2020 00:12:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:12:26: #1 finished! INFO @ Tue, 30 Jun 2020 00:12:26: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:12:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:12:26: 18000000 INFO @ Tue, 30 Jun 2020 00:12:27: #2 number of paired peaks: 51 WARNING @ Tue, 30 Jun 2020 00:12:27: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:12:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:12:33: 19000000 INFO @ Tue, 30 Jun 2020 00:12:39: 20000000 INFO @ Tue, 30 Jun 2020 00:12:43: #1 tag size is determined as 74 bps INFO @ Tue, 30 Jun 2020 00:12:43: #1 tag size = 74 INFO @ Tue, 30 Jun 2020 00:12:43: #1 total tags in treatment: 20630854 INFO @ Tue, 30 Jun 2020 00:12:43: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:12:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:12:43: #1 tags after filtering in treatment: 20630854 INFO @ Tue, 30 Jun 2020 00:12:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:12:43: #1 finished! INFO @ Tue, 30 Jun 2020 00:12:43: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:12:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:12:45: #2 number of paired peaks: 51 WARNING @ Tue, 30 Jun 2020 00:12:45: Too few paired peaks (51) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:12:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287831/SRX5287831.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。