Job ID = 6528295 SRX = SRX5287828 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:38:43 prefetch.2.10.7: 1) Downloading 'SRR8483059'... 2020-06-29T14:38:43 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:41:59 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:42:00 prefetch.2.10.7: 'SRR8483059' is valid 2020-06-29T14:42:00 prefetch.2.10.7: 1) 'SRR8483059' was downloaded successfully 2020-06-29T14:42:00 prefetch.2.10.7: 'SRR8483059' has 0 unresolved dependencies Read 17954539 spots for SRR8483059/SRR8483059.sra Written 17954539 spots for SRR8483059/SRR8483059.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:15 17954539 reads; of these: 17954539 (100.00%) were unpaired; of these: 624739 (3.48%) aligned 0 times 12855731 (71.60%) aligned exactly 1 time 4474069 (24.92%) aligned >1 times 96.52% overall alignment rate Time searching: 00:08:15 Overall time: 00:08:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1778141 / 17329800 = 0.1026 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:03:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:03:12: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:03:12: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:03:18: 1000000 INFO @ Tue, 30 Jun 2020 00:03:23: 2000000 INFO @ Tue, 30 Jun 2020 00:03:29: 3000000 INFO @ Tue, 30 Jun 2020 00:03:34: 4000000 INFO @ Tue, 30 Jun 2020 00:03:39: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:03:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:03:42: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:03:42: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:03:45: 6000000 INFO @ Tue, 30 Jun 2020 00:03:49: 1000000 INFO @ Tue, 30 Jun 2020 00:03:51: 7000000 INFO @ Tue, 30 Jun 2020 00:03:56: 2000000 INFO @ Tue, 30 Jun 2020 00:03:57: 8000000 INFO @ Tue, 30 Jun 2020 00:04:02: 3000000 INFO @ Tue, 30 Jun 2020 00:04:03: 9000000 INFO @ Tue, 30 Jun 2020 00:04:08: 10000000 INFO @ Tue, 30 Jun 2020 00:04:09: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:04:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:04:12: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:04:12: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:04:14: 11000000 INFO @ Tue, 30 Jun 2020 00:04:15: 5000000 INFO @ Tue, 30 Jun 2020 00:04:19: 1000000 INFO @ Tue, 30 Jun 2020 00:04:20: 12000000 INFO @ Tue, 30 Jun 2020 00:04:22: 6000000 INFO @ Tue, 30 Jun 2020 00:04:26: 2000000 INFO @ Tue, 30 Jun 2020 00:04:26: 13000000 INFO @ Tue, 30 Jun 2020 00:04:29: 7000000 INFO @ Tue, 30 Jun 2020 00:04:32: 14000000 INFO @ Tue, 30 Jun 2020 00:04:33: 3000000 INFO @ Tue, 30 Jun 2020 00:04:36: 8000000 INFO @ Tue, 30 Jun 2020 00:04:38: 15000000 INFO @ Tue, 30 Jun 2020 00:04:40: 4000000 INFO @ Tue, 30 Jun 2020 00:04:42: #1 tag size is determined as 73 bps INFO @ Tue, 30 Jun 2020 00:04:42: #1 tag size = 73 INFO @ Tue, 30 Jun 2020 00:04:42: #1 total tags in treatment: 15551659 INFO @ Tue, 30 Jun 2020 00:04:42: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:04:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:04:42: #1 tags after filtering in treatment: 15551659 INFO @ Tue, 30 Jun 2020 00:04:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:04:42: #1 finished! INFO @ Tue, 30 Jun 2020 00:04:42: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:04:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:04:43: 9000000 INFO @ Tue, 30 Jun 2020 00:04:43: #2 number of paired peaks: 27 WARNING @ Tue, 30 Jun 2020 00:04:43: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:04:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:04:47: 5000000 INFO @ Tue, 30 Jun 2020 00:04:49: 10000000 INFO @ Tue, 30 Jun 2020 00:04:54: 6000000 INFO @ Tue, 30 Jun 2020 00:04:56: 11000000 INFO @ Tue, 30 Jun 2020 00:05:01: 7000000 INFO @ Tue, 30 Jun 2020 00:05:03: 12000000 INFO @ Tue, 30 Jun 2020 00:05:07: 8000000 INFO @ Tue, 30 Jun 2020 00:05:10: 13000000 INFO @ Tue, 30 Jun 2020 00:05:14: 9000000 INFO @ Tue, 30 Jun 2020 00:05:17: 14000000 INFO @ Tue, 30 Jun 2020 00:05:21: 10000000 INFO @ Tue, 30 Jun 2020 00:05:25: 15000000 INFO @ Tue, 30 Jun 2020 00:05:27: 11000000 INFO @ Tue, 30 Jun 2020 00:05:29: #1 tag size is determined as 73 bps INFO @ Tue, 30 Jun 2020 00:05:29: #1 tag size = 73 INFO @ Tue, 30 Jun 2020 00:05:29: #1 total tags in treatment: 15551659 INFO @ Tue, 30 Jun 2020 00:05:29: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:05:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:05:29: #1 tags after filtering in treatment: 15551659 INFO @ Tue, 30 Jun 2020 00:05:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:05:29: #1 finished! INFO @ Tue, 30 Jun 2020 00:05:29: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:05:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:05:30: #2 number of paired peaks: 27 WARNING @ Tue, 30 Jun 2020 00:05:30: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:05:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:05:34: 12000000 INFO @ Tue, 30 Jun 2020 00:05:41: 13000000 INFO @ Tue, 30 Jun 2020 00:05:48: 14000000 INFO @ Tue, 30 Jun 2020 00:05:55: 15000000 INFO @ Tue, 30 Jun 2020 00:05:58: #1 tag size is determined as 73 bps INFO @ Tue, 30 Jun 2020 00:05:58: #1 tag size = 73 INFO @ Tue, 30 Jun 2020 00:05:58: #1 total tags in treatment: 15551659 INFO @ Tue, 30 Jun 2020 00:05:58: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:05:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:05:59: #1 tags after filtering in treatment: 15551659 INFO @ Tue, 30 Jun 2020 00:05:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:05:59: #1 finished! INFO @ Tue, 30 Jun 2020 00:05:59: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:05:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:06:00: #2 number of paired peaks: 27 WARNING @ Tue, 30 Jun 2020 00:06:00: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:06:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5287828/SRX5287828.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。