Job ID = 5720783 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T16:29:59 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 24,718,173 reads read : 49,436,346 reads written : 49,436,346 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:09:06 24718173 reads; of these: 24718173 (100.00%) were paired; of these: 4366918 (17.67%) aligned concordantly 0 times 15089047 (61.04%) aligned concordantly exactly 1 time 5262208 (21.29%) aligned concordantly >1 times ---- 4366918 pairs aligned concordantly 0 times; of these: 1420671 (32.53%) aligned discordantly 1 time ---- 2946247 pairs aligned 0 times concordantly or discordantly; of these: 5892494 mates make up the pairs; of these: 2780730 (47.19%) aligned 0 times 1577798 (26.78%) aligned exactly 1 time 1533966 (26.03%) aligned >1 times 94.38% overall alignment rate Time searching: 01:09:06 Overall time: 01:09:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4255877 / 20862895 = 0.2040 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 03:12:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 03:12:45: #1 read tag files... INFO @ Thu, 16 Apr 2020 03:12:45: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 03:12:50: 1000000 INFO @ Thu, 16 Apr 2020 03:12:55: 2000000 INFO @ Thu, 16 Apr 2020 03:13:01: 3000000 INFO @ Thu, 16 Apr 2020 03:13:06: 4000000 INFO @ Thu, 16 Apr 2020 03:13:11: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 03:13:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 03:13:14: #1 read tag files... INFO @ Thu, 16 Apr 2020 03:13:14: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 03:13:16: 6000000 INFO @ Thu, 16 Apr 2020 03:13:20: 1000000 INFO @ Thu, 16 Apr 2020 03:13:22: 7000000 INFO @ Thu, 16 Apr 2020 03:13:25: 2000000 INFO @ Thu, 16 Apr 2020 03:13:27: 8000000 INFO @ Thu, 16 Apr 2020 03:13:31: 3000000 INFO @ Thu, 16 Apr 2020 03:13:33: 9000000 INFO @ Thu, 16 Apr 2020 03:13:37: 4000000 INFO @ Thu, 16 Apr 2020 03:13:38: 10000000 INFO @ Thu, 16 Apr 2020 03:13:42: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 03:13:43: 11000000 INFO @ Thu, 16 Apr 2020 03:13:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 03:13:44: #1 read tag files... INFO @ Thu, 16 Apr 2020 03:13:44: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 03:13:48: 6000000 INFO @ Thu, 16 Apr 2020 03:13:49: 12000000 INFO @ Thu, 16 Apr 2020 03:13:50: 1000000 INFO @ Thu, 16 Apr 2020 03:13:53: 7000000 INFO @ Thu, 16 Apr 2020 03:13:54: 13000000 INFO @ Thu, 16 Apr 2020 03:13:55: 2000000 INFO @ Thu, 16 Apr 2020 03:13:59: 8000000 INFO @ Thu, 16 Apr 2020 03:14:00: 14000000 INFO @ Thu, 16 Apr 2020 03:14:01: 3000000 INFO @ Thu, 16 Apr 2020 03:14:05: 9000000 INFO @ Thu, 16 Apr 2020 03:14:05: 15000000 INFO @ Thu, 16 Apr 2020 03:14:06: 4000000 INFO @ Thu, 16 Apr 2020 03:14:10: 10000000 INFO @ Thu, 16 Apr 2020 03:14:11: 16000000 INFO @ Thu, 16 Apr 2020 03:14:11: 5000000 INFO @ Thu, 16 Apr 2020 03:14:16: 11000000 INFO @ Thu, 16 Apr 2020 03:14:16: 17000000 INFO @ Thu, 16 Apr 2020 03:14:17: 6000000 INFO @ Thu, 16 Apr 2020 03:14:21: 12000000 INFO @ Thu, 16 Apr 2020 03:14:22: 18000000 INFO @ Thu, 16 Apr 2020 03:14:22: 7000000 INFO @ Thu, 16 Apr 2020 03:14:27: 13000000 INFO @ Thu, 16 Apr 2020 03:14:27: 8000000 INFO @ Thu, 16 Apr 2020 03:14:28: 19000000 INFO @ Thu, 16 Apr 2020 03:14:32: 14000000 INFO @ Thu, 16 Apr 2020 03:14:33: 9000000 INFO @ Thu, 16 Apr 2020 03:14:33: 20000000 INFO @ Thu, 16 Apr 2020 03:14:38: 15000000 INFO @ Thu, 16 Apr 2020 03:14:38: 10000000 INFO @ Thu, 16 Apr 2020 03:14:38: 21000000 INFO @ Thu, 16 Apr 2020 03:14:43: 11000000 INFO @ Thu, 16 Apr 2020 03:14:43: 16000000 INFO @ Thu, 16 Apr 2020 03:14:44: 22000000 INFO @ Thu, 16 Apr 2020 03:14:48: 12000000 INFO @ Thu, 16 Apr 2020 03:14:49: 17000000 INFO @ Thu, 16 Apr 2020 03:14:49: 23000000 INFO @ Thu, 16 Apr 2020 03:14:54: 13000000 INFO @ Thu, 16 Apr 2020 03:14:54: 18000000 INFO @ Thu, 16 Apr 2020 03:14:55: 24000000 INFO @ Thu, 16 Apr 2020 03:14:59: 14000000 INFO @ Thu, 16 Apr 2020 03:15:00: 25000000 INFO @ Thu, 16 Apr 2020 03:15:00: 19000000 INFO @ Thu, 16 Apr 2020 03:15:04: 15000000 INFO @ Thu, 16 Apr 2020 03:15:05: 26000000 INFO @ Thu, 16 Apr 2020 03:15:06: 20000000 INFO @ Thu, 16 Apr 2020 03:15:10: 16000000 INFO @ Thu, 16 Apr 2020 03:15:10: 27000000 INFO @ Thu, 16 Apr 2020 03:15:11: 21000000 INFO @ Thu, 16 Apr 2020 03:15:15: 17000000 INFO @ Thu, 16 Apr 2020 03:15:16: 28000000 INFO @ Thu, 16 Apr 2020 03:15:17: 22000000 INFO @ Thu, 16 Apr 2020 03:15:20: 18000000 INFO @ Thu, 16 Apr 2020 03:15:21: 29000000 INFO @ Thu, 16 Apr 2020 03:15:22: 23000000 INFO @ Thu, 16 Apr 2020 03:15:26: 19000000 INFO @ Thu, 16 Apr 2020 03:15:26: 30000000 INFO @ Thu, 16 Apr 2020 03:15:28: 24000000 INFO @ Thu, 16 Apr 2020 03:15:31: 20000000 INFO @ Thu, 16 Apr 2020 03:15:32: 31000000 INFO @ Thu, 16 Apr 2020 03:15:33: 25000000 INFO @ Thu, 16 Apr 2020 03:15:36: 21000000 INFO @ Thu, 16 Apr 2020 03:15:37: 32000000 INFO @ Thu, 16 Apr 2020 03:15:38: 26000000 INFO @ Thu, 16 Apr 2020 03:15:41: 22000000 INFO @ Thu, 16 Apr 2020 03:15:43: 33000000 INFO @ Thu, 16 Apr 2020 03:15:43: 27000000 INFO @ Thu, 16 Apr 2020 03:15:47: 23000000 INFO @ Thu, 16 Apr 2020 03:15:49: 28000000 INFO @ Thu, 16 Apr 2020 03:15:49: 34000000 INFO @ Thu, 16 Apr 2020 03:15:52: 24000000 INFO @ Thu, 16 Apr 2020 03:15:54: 29000000 INFO @ Thu, 16 Apr 2020 03:15:54: 35000000 INFO @ Thu, 16 Apr 2020 03:15:57: 25000000 INFO @ Thu, 16 Apr 2020 03:15:59: 30000000 INFO @ Thu, 16 Apr 2020 03:16:00: 36000000 INFO @ Thu, 16 Apr 2020 03:16:02: 26000000 INFO @ Thu, 16 Apr 2020 03:16:05: 31000000 INFO @ Thu, 16 Apr 2020 03:16:05: 37000000 INFO @ Thu, 16 Apr 2020 03:16:07: 27000000 INFO @ Thu, 16 Apr 2020 03:16:11: 38000000 INFO @ Thu, 16 Apr 2020 03:16:11: 32000000 INFO @ Thu, 16 Apr 2020 03:16:12: #1 tag size is determined as 101 bps INFO @ Thu, 16 Apr 2020 03:16:12: #1 tag size = 101 INFO @ Thu, 16 Apr 2020 03:16:12: #1 total tags in treatment: 16198476 INFO @ Thu, 16 Apr 2020 03:16:12: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 03:16:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 03:16:12: #1 tags after filtering in treatment: 15233048 INFO @ Thu, 16 Apr 2020 03:16:12: #1 Redundant rate of treatment: 0.06 INFO @ Thu, 16 Apr 2020 03:16:12: #1 finished! INFO @ Thu, 16 Apr 2020 03:16:12: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 03:16:12: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 03:16:12: 28000000 INFO @ Thu, 16 Apr 2020 03:16:13: #2 number of paired peaks: 179 WARNING @ Thu, 16 Apr 2020 03:16:13: Fewer paired peaks (179) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 179 pairs to build model! INFO @ Thu, 16 Apr 2020 03:16:13: start model_add_line... INFO @ Thu, 16 Apr 2020 03:16:13: start X-correlation... INFO @ Thu, 16 Apr 2020 03:16:13: end of X-cor INFO @ Thu, 16 Apr 2020 03:16:13: #2 finished! INFO @ Thu, 16 Apr 2020 03:16:13: #2 predicted fragment length is 164 bps INFO @ Thu, 16 Apr 2020 03:16:13: #2 alternative fragment length(s) may be 164 bps INFO @ Thu, 16 Apr 2020 03:16:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.05_model.r WARNING @ Thu, 16 Apr 2020 03:16:13: #2 Since the d (164) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 03:16:13: #2 You may need to consider one of the other alternative d(s): 164 WARNING @ Thu, 16 Apr 2020 03:16:13: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 03:16:13: #3 Call peaks... INFO @ Thu, 16 Apr 2020 03:16:13: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 03:16:16: 33000000 INFO @ Thu, 16 Apr 2020 03:16:17: 29000000 INFO @ Thu, 16 Apr 2020 03:16:22: 34000000 INFO @ Thu, 16 Apr 2020 03:16:23: 30000000 INFO @ Thu, 16 Apr 2020 03:16:27: 35000000 INFO @ Thu, 16 Apr 2020 03:16:28: 31000000 INFO @ Thu, 16 Apr 2020 03:16:32: 36000000 INFO @ Thu, 16 Apr 2020 03:16:33: 32000000 INFO @ Thu, 16 Apr 2020 03:16:37: 37000000 INFO @ Thu, 16 Apr 2020 03:16:39: 33000000 INFO @ Thu, 16 Apr 2020 03:16:42: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 03:16:43: 38000000 INFO @ Thu, 16 Apr 2020 03:16:44: #1 tag size is determined as 101 bps INFO @ Thu, 16 Apr 2020 03:16:44: #1 tag size = 101 INFO @ Thu, 16 Apr 2020 03:16:44: #1 total tags in treatment: 16198476 INFO @ Thu, 16 Apr 2020 03:16:44: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 03:16:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 03:16:44: #1 tags after filtering in treatment: 15233048 INFO @ Thu, 16 Apr 2020 03:16:44: #1 Redundant rate of treatment: 0.06 INFO @ Thu, 16 Apr 2020 03:16:44: #1 finished! INFO @ Thu, 16 Apr 2020 03:16:44: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 03:16:44: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 03:16:44: 34000000 INFO @ Thu, 16 Apr 2020 03:16:45: #2 number of paired peaks: 179 WARNING @ Thu, 16 Apr 2020 03:16:45: Fewer paired peaks (179) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 179 pairs to build model! INFO @ Thu, 16 Apr 2020 03:16:45: start model_add_line... INFO @ Thu, 16 Apr 2020 03:16:45: start X-correlation... INFO @ Thu, 16 Apr 2020 03:16:45: end of X-cor INFO @ Thu, 16 Apr 2020 03:16:45: #2 finished! INFO @ Thu, 16 Apr 2020 03:16:45: #2 predicted fragment length is 164 bps INFO @ Thu, 16 Apr 2020 03:16:45: #2 alternative fragment length(s) may be 164 bps INFO @ Thu, 16 Apr 2020 03:16:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.10_model.r WARNING @ Thu, 16 Apr 2020 03:16:45: #2 Since the d (164) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 03:16:45: #2 You may need to consider one of the other alternative d(s): 164 WARNING @ Thu, 16 Apr 2020 03:16:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 03:16:45: #3 Call peaks... INFO @ Thu, 16 Apr 2020 03:16:45: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 03:16:49: 35000000 INFO @ Thu, 16 Apr 2020 03:16:54: 36000000 INFO @ Thu, 16 Apr 2020 03:16:57: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.05_peaks.xls INFO @ Thu, 16 Apr 2020 03:16:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 03:16:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.05_summits.bed INFO @ Thu, 16 Apr 2020 03:16:57: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (2976 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 03:16:59: 37000000 INFO @ Thu, 16 Apr 2020 03:17:05: 38000000 INFO @ Thu, 16 Apr 2020 03:17:05: #1 tag size is determined as 101 bps INFO @ Thu, 16 Apr 2020 03:17:05: #1 tag size = 101 INFO @ Thu, 16 Apr 2020 03:17:05: #1 total tags in treatment: 16198476 INFO @ Thu, 16 Apr 2020 03:17:05: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 03:17:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 03:17:06: #1 tags after filtering in treatment: 15233048 INFO @ Thu, 16 Apr 2020 03:17:06: #1 Redundant rate of treatment: 0.06 INFO @ Thu, 16 Apr 2020 03:17:06: #1 finished! INFO @ Thu, 16 Apr 2020 03:17:06: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 03:17:06: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 03:17:07: #2 number of paired peaks: 179 WARNING @ Thu, 16 Apr 2020 03:17:07: Fewer paired peaks (179) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 179 pairs to build model! INFO @ Thu, 16 Apr 2020 03:17:07: start model_add_line... INFO @ Thu, 16 Apr 2020 03:17:07: start X-correlation... INFO @ Thu, 16 Apr 2020 03:17:07: end of X-cor INFO @ Thu, 16 Apr 2020 03:17:07: #2 finished! INFO @ Thu, 16 Apr 2020 03:17:07: #2 predicted fragment length is 164 bps INFO @ Thu, 16 Apr 2020 03:17:07: #2 alternative fragment length(s) may be 164 bps INFO @ Thu, 16 Apr 2020 03:17:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.20_model.r WARNING @ Thu, 16 Apr 2020 03:17:07: #2 Since the d (164) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 03:17:07: #2 You may need to consider one of the other alternative d(s): 164 WARNING @ Thu, 16 Apr 2020 03:17:07: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 03:17:07: #3 Call peaks... INFO @ Thu, 16 Apr 2020 03:17:07: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 03:17:13: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 03:17:28: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.10_peaks.xls INFO @ Thu, 16 Apr 2020 03:17:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 03:17:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.10_summits.bed INFO @ Thu, 16 Apr 2020 03:17:28: Done! pass1 - making usageList (14 chroms): 0 millis pass2 - checking and writing primary data (1823 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 03:17:36: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 03:17:51: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.20_peaks.xls INFO @ Thu, 16 Apr 2020 03:17:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 03:17:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX5246876/SRX5246876.20_summits.bed INFO @ Thu, 16 Apr 2020 03:17:51: Done! pass1 - making usageList (13 chroms): 0 millis pass2 - checking and writing primary data (1006 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。