Job ID = 6528285 SRX = SRX5243664 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:32:21 prefetch.2.10.7: 1) Downloading 'SRR8436121'... 2020-06-29T14:32:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:34:31 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:34:32 prefetch.2.10.7: 'SRR8436121' is valid 2020-06-29T14:34:32 prefetch.2.10.7: 1) 'SRR8436121' was downloaded successfully Read 8266580 spots for SRR8436121/SRR8436121.sra Written 8266580 spots for SRR8436121/SRR8436121.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:21 8266580 reads; of these: 8266580 (100.00%) were unpaired; of these: 408816 (4.95%) aligned 0 times 6229472 (75.36%) aligned exactly 1 time 1628292 (19.70%) aligned >1 times 95.05% overall alignment rate Time searching: 00:02:21 Overall time: 00:02:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1193034 / 7857764 = 0.1518 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:41:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:41:32: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:41:32: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:41:37: 1000000 INFO @ Mon, 29 Jun 2020 23:41:42: 2000000 INFO @ Mon, 29 Jun 2020 23:41:47: 3000000 INFO @ Mon, 29 Jun 2020 23:41:52: 4000000 INFO @ Mon, 29 Jun 2020 23:41:57: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:42:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:42:02: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:42:02: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:42:02: 6000000 INFO @ Mon, 29 Jun 2020 23:42:06: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:42:06: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:42:06: #1 total tags in treatment: 6664730 INFO @ Mon, 29 Jun 2020 23:42:06: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:42:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:42:06: #1 tags after filtering in treatment: 6664730 INFO @ Mon, 29 Jun 2020 23:42:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:42:06: #1 finished! INFO @ Mon, 29 Jun 2020 23:42:06: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:42:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:42:06: #2 number of paired peaks: 36 WARNING @ Mon, 29 Jun 2020 23:42:06: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:42:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:42:07: 1000000 INFO @ Mon, 29 Jun 2020 23:42:13: 2000000 INFO @ Mon, 29 Jun 2020 23:42:18: 3000000 INFO @ Mon, 29 Jun 2020 23:42:23: 4000000 INFO @ Mon, 29 Jun 2020 23:42:28: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:42:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:42:32: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:42:32: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:42:33: 6000000 INFO @ Mon, 29 Jun 2020 23:42:37: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:42:37: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:42:37: #1 total tags in treatment: 6664730 INFO @ Mon, 29 Jun 2020 23:42:37: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:42:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:42:37: #1 tags after filtering in treatment: 6664730 INFO @ Mon, 29 Jun 2020 23:42:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:42:37: #1 finished! INFO @ Mon, 29 Jun 2020 23:42:37: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:42:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:42:37: 1000000 INFO @ Mon, 29 Jun 2020 23:42:38: #2 number of paired peaks: 36 WARNING @ Mon, 29 Jun 2020 23:42:38: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:42:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:42:43: 2000000 INFO @ Mon, 29 Jun 2020 23:42:48: 3000000 INFO @ Mon, 29 Jun 2020 23:42:53: 4000000 INFO @ Mon, 29 Jun 2020 23:42:59: 5000000 INFO @ Mon, 29 Jun 2020 23:43:04: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:43:08: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:43:08: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:43:08: #1 total tags in treatment: 6664730 INFO @ Mon, 29 Jun 2020 23:43:08: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:43:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:43:08: #1 tags after filtering in treatment: 6664730 INFO @ Mon, 29 Jun 2020 23:43:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:43:08: #1 finished! INFO @ Mon, 29 Jun 2020 23:43:08: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:43:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:43:08: #2 number of paired peaks: 36 WARNING @ Mon, 29 Jun 2020 23:43:08: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:43:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5243664/SRX5243664.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。