Job ID = 5720759 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 8,155,678 reads read : 16,311,356 reads written : 8,155,678 reads 0-length : 8,155,678 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:40 8155678 reads; of these: 8155678 (100.00%) were unpaired; of these: 2004491 (24.58%) aligned 0 times 1622955 (19.90%) aligned exactly 1 time 4528232 (55.52%) aligned >1 times 75.42% overall alignment rate Time searching: 00:06:40 Overall time: 00:06:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 2369295 / 6151187 = 0.3852 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 01:25:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 01:25:38: #1 read tag files... INFO @ Thu, 16 Apr 2020 01:25:38: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 01:25:44: 1000000 INFO @ Thu, 16 Apr 2020 01:25:50: 2000000 INFO @ Thu, 16 Apr 2020 01:25:56: 3000000 INFO @ Thu, 16 Apr 2020 01:26:01: #1 tag size is determined as 75 bps INFO @ Thu, 16 Apr 2020 01:26:01: #1 tag size = 75 INFO @ Thu, 16 Apr 2020 01:26:01: #1 total tags in treatment: 3781892 INFO @ Thu, 16 Apr 2020 01:26:01: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 01:26:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 01:26:01: #1 tags after filtering in treatment: 3781892 INFO @ Thu, 16 Apr 2020 01:26:01: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 01:26:01: #1 finished! INFO @ Thu, 16 Apr 2020 01:26:01: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 01:26:01: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 01:26:02: #2 number of paired peaks: 3909 INFO @ Thu, 16 Apr 2020 01:26:02: start model_add_line... INFO @ Thu, 16 Apr 2020 01:26:02: start X-correlation... INFO @ Thu, 16 Apr 2020 01:26:02: end of X-cor INFO @ Thu, 16 Apr 2020 01:26:02: #2 finished! INFO @ Thu, 16 Apr 2020 01:26:02: #2 predicted fragment length is 92 bps INFO @ Thu, 16 Apr 2020 01:26:02: #2 alternative fragment length(s) may be 92 bps INFO @ Thu, 16 Apr 2020 01:26:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.05_model.r WARNING @ Thu, 16 Apr 2020 01:26:02: #2 Since the d (92) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 01:26:02: #2 You may need to consider one of the other alternative d(s): 92 WARNING @ Thu, 16 Apr 2020 01:26:02: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 01:26:02: #3 Call peaks... INFO @ Thu, 16 Apr 2020 01:26:02: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 01:26:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 01:26:08: #1 read tag files... INFO @ Thu, 16 Apr 2020 01:26:08: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 01:26:10: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 01:26:14: 1000000 INFO @ Thu, 16 Apr 2020 01:26:14: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.05_peaks.xls INFO @ Thu, 16 Apr 2020 01:26:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 01:26:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.05_summits.bed INFO @ Thu, 16 Apr 2020 01:26:15: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (5147 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 01:26:20: 2000000 INFO @ Thu, 16 Apr 2020 01:26:26: 3000000 INFO @ Thu, 16 Apr 2020 01:26:31: #1 tag size is determined as 75 bps INFO @ Thu, 16 Apr 2020 01:26:31: #1 tag size = 75 INFO @ Thu, 16 Apr 2020 01:26:31: #1 total tags in treatment: 3781892 INFO @ Thu, 16 Apr 2020 01:26:31: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 01:26:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 01:26:31: #1 tags after filtering in treatment: 3781892 INFO @ Thu, 16 Apr 2020 01:26:31: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 01:26:31: #1 finished! INFO @ Thu, 16 Apr 2020 01:26:31: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 01:26:31: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 01:26:32: #2 number of paired peaks: 3909 INFO @ Thu, 16 Apr 2020 01:26:32: start model_add_line... INFO @ Thu, 16 Apr 2020 01:26:32: start X-correlation... INFO @ Thu, 16 Apr 2020 01:26:32: end of X-cor INFO @ Thu, 16 Apr 2020 01:26:32: #2 finished! INFO @ Thu, 16 Apr 2020 01:26:32: #2 predicted fragment length is 92 bps INFO @ Thu, 16 Apr 2020 01:26:32: #2 alternative fragment length(s) may be 92 bps INFO @ Thu, 16 Apr 2020 01:26:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.10_model.r WARNING @ Thu, 16 Apr 2020 01:26:32: #2 Since the d (92) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 01:26:32: #2 You may need to consider one of the other alternative d(s): 92 WARNING @ Thu, 16 Apr 2020 01:26:32: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 01:26:32: #3 Call peaks... INFO @ Thu, 16 Apr 2020 01:26:32: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 01:26:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 01:26:38: #1 read tag files... INFO @ Thu, 16 Apr 2020 01:26:38: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 01:26:40: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 01:26:44: 1000000 INFO @ Thu, 16 Apr 2020 01:26:44: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.10_peaks.xls INFO @ Thu, 16 Apr 2020 01:26:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 01:26:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.10_summits.bed INFO @ Thu, 16 Apr 2020 01:26:44: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (3123 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 01:26:50: 2000000 INFO @ Thu, 16 Apr 2020 01:26:57: 3000000 INFO @ Thu, 16 Apr 2020 01:27:02: #1 tag size is determined as 75 bps INFO @ Thu, 16 Apr 2020 01:27:02: #1 tag size = 75 INFO @ Thu, 16 Apr 2020 01:27:02: #1 total tags in treatment: 3781892 INFO @ Thu, 16 Apr 2020 01:27:02: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 01:27:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 01:27:02: #1 tags after filtering in treatment: 3781892 INFO @ Thu, 16 Apr 2020 01:27:02: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 01:27:02: #1 finished! INFO @ Thu, 16 Apr 2020 01:27:02: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 01:27:02: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 01:27:02: #2 number of paired peaks: 3909 INFO @ Thu, 16 Apr 2020 01:27:02: start model_add_line... INFO @ Thu, 16 Apr 2020 01:27:02: start X-correlation... INFO @ Thu, 16 Apr 2020 01:27:02: end of X-cor INFO @ Thu, 16 Apr 2020 01:27:02: #2 finished! INFO @ Thu, 16 Apr 2020 01:27:02: #2 predicted fragment length is 92 bps INFO @ Thu, 16 Apr 2020 01:27:02: #2 alternative fragment length(s) may be 92 bps INFO @ Thu, 16 Apr 2020 01:27:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.20_model.r WARNING @ Thu, 16 Apr 2020 01:27:02: #2 Since the d (92) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 01:27:02: #2 You may need to consider one of the other alternative d(s): 92 WARNING @ Thu, 16 Apr 2020 01:27:02: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 01:27:02: #3 Call peaks... INFO @ Thu, 16 Apr 2020 01:27:02: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 01:27:11: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 01:27:15: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.20_peaks.xls INFO @ Thu, 16 Apr 2020 01:27:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 01:27:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX5226996/SRX5226996.20_summits.bed INFO @ Thu, 16 Apr 2020 01:27:15: Done! pass1 - making usageList (14 chroms): 0 millis pass2 - checking and writing primary data (1718 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。