Job ID = 6528281 SRX = SRX511126 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:54:44 prefetch.2.10.7: 1) Downloading 'SRR1217231'... 2020-06-29T14:54:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:56:22 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:56:23 prefetch.2.10.7: 'SRR1217231' is valid 2020-06-29T14:56:23 prefetch.2.10.7: 1) 'SRR1217231' was downloaded successfully Read 10401617 spots for SRR1217231/SRR1217231.sra Written 10401617 spots for SRR1217231/SRR1217231.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:04:32 10401617 reads; of these: 10401617 (100.00%) were unpaired; of these: 170874 (1.64%) aligned 0 times 8057959 (77.47%) aligned exactly 1 time 2172784 (20.89%) aligned >1 times 98.36% overall alignment rate Time searching: 00:04:33 Overall time: 00:04:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 578697 / 10230743 = 0.0566 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:08:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:08:41: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:08:41: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:08:49: 1000000 INFO @ Tue, 30 Jun 2020 00:08:56: 2000000 INFO @ Tue, 30 Jun 2020 00:09:04: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:09:11: 4000000 INFO @ Tue, 30 Jun 2020 00:09:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:09:11: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:09:11: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:09:19: 5000000 INFO @ Tue, 30 Jun 2020 00:09:19: 1000000 INFO @ Tue, 30 Jun 2020 00:09:27: 6000000 INFO @ Tue, 30 Jun 2020 00:09:27: 2000000 INFO @ Tue, 30 Jun 2020 00:09:35: 7000000 INFO @ Tue, 30 Jun 2020 00:09:35: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:09:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:09:41: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:09:41: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:09:43: 8000000 INFO @ Tue, 30 Jun 2020 00:09:44: 4000000 INFO @ Tue, 30 Jun 2020 00:09:50: 1000000 INFO @ Tue, 30 Jun 2020 00:09:51: 9000000 INFO @ Tue, 30 Jun 2020 00:09:52: 5000000 INFO @ Tue, 30 Jun 2020 00:09:56: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:09:56: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:09:56: #1 total tags in treatment: 9652046 INFO @ Tue, 30 Jun 2020 00:09:56: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:09:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:09:56: #1 tags after filtering in treatment: 9652046 INFO @ Tue, 30 Jun 2020 00:09:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:09:56: #1 finished! INFO @ Tue, 30 Jun 2020 00:09:56: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:09:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:09:57: #2 number of paired peaks: 64 WARNING @ Tue, 30 Jun 2020 00:09:57: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:09:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:09:58: 2000000 INFO @ Tue, 30 Jun 2020 00:10:00: 6000000 INFO @ Tue, 30 Jun 2020 00:10:06: 3000000 INFO @ Tue, 30 Jun 2020 00:10:08: 7000000 INFO @ Tue, 30 Jun 2020 00:10:14: 4000000 INFO @ Tue, 30 Jun 2020 00:10:16: 8000000 INFO @ Tue, 30 Jun 2020 00:10:21: 5000000 INFO @ Tue, 30 Jun 2020 00:10:24: 9000000 INFO @ Tue, 30 Jun 2020 00:10:29: 6000000 INFO @ Tue, 30 Jun 2020 00:10:29: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:10:29: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:10:29: #1 total tags in treatment: 9652046 INFO @ Tue, 30 Jun 2020 00:10:29: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:10:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:10:30: #1 tags after filtering in treatment: 9652046 INFO @ Tue, 30 Jun 2020 00:10:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:10:30: #1 finished! INFO @ Tue, 30 Jun 2020 00:10:30: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:10:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:10:30: #2 number of paired peaks: 64 WARNING @ Tue, 30 Jun 2020 00:10:30: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:10:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:10:36: 7000000 INFO @ Tue, 30 Jun 2020 00:10:43: 8000000 INFO @ Tue, 30 Jun 2020 00:10:50: 9000000 INFO @ Tue, 30 Jun 2020 00:10:54: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:10:54: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:10:54: #1 total tags in treatment: 9652046 INFO @ Tue, 30 Jun 2020 00:10:54: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:10:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:10:55: #1 tags after filtering in treatment: 9652046 INFO @ Tue, 30 Jun 2020 00:10:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:10:55: #1 finished! INFO @ Tue, 30 Jun 2020 00:10:55: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:10:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:10:55: #2 number of paired peaks: 64 WARNING @ Tue, 30 Jun 2020 00:10:55: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:10:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX511126/SRX511126.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。