Job ID = 2590656 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 67,994,326 reads read : 67,994,326 reads written : 67,994,326 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:20 67994326 reads; of these: 67994326 (100.00%) were unpaired; of these: 9958718 (14.65%) aligned 0 times 46700234 (68.68%) aligned exactly 1 time 11335374 (16.67%) aligned >1 times 85.35% overall alignment rate Time searching: 00:19:20 Overall time: 00:19:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 22298973 / 58035608 = 0.3842 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 13 Aug 2019 00:00:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 00:00:18: #1 read tag files... INFO @ Tue, 13 Aug 2019 00:00:18: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 00:00:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 00:00:19: #1 read tag files... INFO @ Tue, 13 Aug 2019 00:00:19: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 00:00:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 00:00:20: #1 read tag files... INFO @ Tue, 13 Aug 2019 00:00:20: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 00:00:26: 1000000 INFO @ Tue, 13 Aug 2019 00:00:29: 1000000 INFO @ Tue, 13 Aug 2019 00:00:30: 1000000 INFO @ Tue, 13 Aug 2019 00:00:33: 2000000 INFO @ Tue, 13 Aug 2019 00:00:40: 2000000 INFO @ Tue, 13 Aug 2019 00:00:41: 2000000 INFO @ Tue, 13 Aug 2019 00:00:41: 3000000 INFO @ Tue, 13 Aug 2019 00:00:49: 4000000 INFO @ Tue, 13 Aug 2019 00:00:50: 3000000 INFO @ Tue, 13 Aug 2019 00:00:51: 3000000 INFO @ Tue, 13 Aug 2019 00:00:56: 5000000 INFO @ Tue, 13 Aug 2019 00:01:00: 4000000 INFO @ Tue, 13 Aug 2019 00:01:01: 4000000 INFO @ Tue, 13 Aug 2019 00:01:04: 6000000 INFO @ Tue, 13 Aug 2019 00:01:10: 5000000 INFO @ Tue, 13 Aug 2019 00:01:12: 5000000 INFO @ Tue, 13 Aug 2019 00:01:12: 7000000 INFO @ Tue, 13 Aug 2019 00:01:19: 8000000 INFO @ Tue, 13 Aug 2019 00:01:21: 6000000 INFO @ Tue, 13 Aug 2019 00:01:22: 6000000 INFO @ Tue, 13 Aug 2019 00:01:27: 9000000 INFO @ Tue, 13 Aug 2019 00:01:31: 7000000 INFO @ Tue, 13 Aug 2019 00:01:32: 7000000 INFO @ Tue, 13 Aug 2019 00:01:35: 10000000 INFO @ Tue, 13 Aug 2019 00:01:41: 8000000 INFO @ Tue, 13 Aug 2019 00:01:42: 11000000 INFO @ Tue, 13 Aug 2019 00:01:42: 8000000 INFO @ Tue, 13 Aug 2019 00:01:50: 12000000 INFO @ Tue, 13 Aug 2019 00:01:51: 9000000 INFO @ Tue, 13 Aug 2019 00:01:53: 9000000 INFO @ Tue, 13 Aug 2019 00:01:57: 13000000 INFO @ Tue, 13 Aug 2019 00:02:01: 10000000 INFO @ Tue, 13 Aug 2019 00:02:03: 10000000 INFO @ Tue, 13 Aug 2019 00:02:05: 14000000 INFO @ Tue, 13 Aug 2019 00:02:12: 11000000 INFO @ Tue, 13 Aug 2019 00:02:13: 15000000 INFO @ Tue, 13 Aug 2019 00:02:13: 11000000 INFO @ Tue, 13 Aug 2019 00:02:20: 16000000 INFO @ Tue, 13 Aug 2019 00:02:22: 12000000 INFO @ Tue, 13 Aug 2019 00:02:23: 12000000 INFO @ Tue, 13 Aug 2019 00:02:28: 17000000 INFO @ Tue, 13 Aug 2019 00:02:32: 13000000 INFO @ Tue, 13 Aug 2019 00:02:33: 13000000 INFO @ Tue, 13 Aug 2019 00:02:35: 18000000 INFO @ Tue, 13 Aug 2019 00:02:42: 14000000 INFO @ Tue, 13 Aug 2019 00:02:43: 19000000 INFO @ Tue, 13 Aug 2019 00:02:43: 14000000 INFO @ Tue, 13 Aug 2019 00:02:50: 20000000 INFO @ Tue, 13 Aug 2019 00:02:52: 15000000 INFO @ Tue, 13 Aug 2019 00:02:53: 15000000 INFO @ Tue, 13 Aug 2019 00:02:58: 21000000 INFO @ Tue, 13 Aug 2019 00:03:02: 16000000 INFO @ Tue, 13 Aug 2019 00:03:03: 16000000 INFO @ Tue, 13 Aug 2019 00:03:06: 22000000 INFO @ Tue, 13 Aug 2019 00:03:12: 17000000 INFO @ Tue, 13 Aug 2019 00:03:13: 23000000 INFO @ Tue, 13 Aug 2019 00:03:13: 17000000 INFO @ Tue, 13 Aug 2019 00:03:21: 24000000 INFO @ Tue, 13 Aug 2019 00:03:22: 18000000 INFO @ Tue, 13 Aug 2019 00:03:23: 18000000 INFO @ Tue, 13 Aug 2019 00:03:28: 25000000 INFO @ Tue, 13 Aug 2019 00:03:32: 19000000 INFO @ Tue, 13 Aug 2019 00:03:33: 19000000 INFO @ Tue, 13 Aug 2019 00:03:36: 26000000 INFO @ Tue, 13 Aug 2019 00:03:42: 20000000 INFO @ Tue, 13 Aug 2019 00:03:43: 20000000 INFO @ Tue, 13 Aug 2019 00:03:44: 27000000 INFO @ Tue, 13 Aug 2019 00:03:51: 28000000 INFO @ Tue, 13 Aug 2019 00:03:52: 21000000 INFO @ Tue, 13 Aug 2019 00:03:53: 21000000 INFO @ Tue, 13 Aug 2019 00:03:59: 29000000 INFO @ Tue, 13 Aug 2019 00:04:02: 22000000 INFO @ Tue, 13 Aug 2019 00:04:03: 22000000 INFO @ Tue, 13 Aug 2019 00:04:07: 30000000 INFO @ Tue, 13 Aug 2019 00:04:12: 23000000 INFO @ Tue, 13 Aug 2019 00:04:13: 23000000 INFO @ Tue, 13 Aug 2019 00:04:14: 31000000 INFO @ Tue, 13 Aug 2019 00:04:22: 32000000 INFO @ Tue, 13 Aug 2019 00:04:22: 24000000 INFO @ Tue, 13 Aug 2019 00:04:23: 24000000 INFO @ Tue, 13 Aug 2019 00:04:29: 33000000 INFO @ Tue, 13 Aug 2019 00:04:32: 25000000 INFO @ Tue, 13 Aug 2019 00:04:33: 25000000 INFO @ Tue, 13 Aug 2019 00:04:37: 34000000 INFO @ Tue, 13 Aug 2019 00:04:42: 26000000 INFO @ Tue, 13 Aug 2019 00:04:44: 26000000 INFO @ Tue, 13 Aug 2019 00:04:44: 35000000 INFO @ Tue, 13 Aug 2019 00:04:50: #1 tag size is determined as 50 bps INFO @ Tue, 13 Aug 2019 00:04:50: #1 tag size = 50 INFO @ Tue, 13 Aug 2019 00:04:50: #1 total tags in treatment: 35736635 INFO @ Tue, 13 Aug 2019 00:04:50: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 00:04:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 00:04:51: #1 tags after filtering in treatment: 35736635 INFO @ Tue, 13 Aug 2019 00:04:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 00:04:51: #1 finished! INFO @ Tue, 13 Aug 2019 00:04:51: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 00:04:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 00:04:52: 27000000 INFO @ Tue, 13 Aug 2019 00:04:54: 27000000 INFO @ Tue, 13 Aug 2019 00:04:54: #2 number of paired peaks: 0 WARNING @ Tue, 13 Aug 2019 00:04:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 00:04:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 13 Aug 2019 00:05:03: 28000000 INFO @ Tue, 13 Aug 2019 00:05:04: 28000000 INFO @ Tue, 13 Aug 2019 00:05:13: 29000000 INFO @ Tue, 13 Aug 2019 00:05:14: 29000000 INFO @ Tue, 13 Aug 2019 00:05:23: 30000000 INFO @ Tue, 13 Aug 2019 00:05:24: 30000000 INFO @ Tue, 13 Aug 2019 00:05:33: 31000000 INFO @ Tue, 13 Aug 2019 00:05:34: 31000000 INFO @ Tue, 13 Aug 2019 00:05:43: 32000000 INFO @ Tue, 13 Aug 2019 00:05:44: 32000000 INFO @ Tue, 13 Aug 2019 00:05:53: 33000000 INFO @ Tue, 13 Aug 2019 00:05:54: 33000000 INFO @ Tue, 13 Aug 2019 00:06:03: 34000000 INFO @ Tue, 13 Aug 2019 00:06:04: 34000000 INFO @ Tue, 13 Aug 2019 00:06:13: 35000000 INFO @ Tue, 13 Aug 2019 00:06:14: 35000000 INFO @ Tue, 13 Aug 2019 00:06:20: #1 tag size is determined as 50 bps INFO @ Tue, 13 Aug 2019 00:06:20: #1 tag size = 50 INFO @ Tue, 13 Aug 2019 00:06:20: #1 total tags in treatment: 35736635 INFO @ Tue, 13 Aug 2019 00:06:20: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 00:06:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 00:06:21: #1 tags after filtering in treatment: 35736635 INFO @ Tue, 13 Aug 2019 00:06:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 00:06:21: #1 finished! INFO @ Tue, 13 Aug 2019 00:06:21: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 00:06:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 00:06:21: #1 tag size is determined as 50 bps INFO @ Tue, 13 Aug 2019 00:06:21: #1 tag size = 50 INFO @ Tue, 13 Aug 2019 00:06:21: #1 total tags in treatment: 35736635 INFO @ Tue, 13 Aug 2019 00:06:21: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 00:06:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 00:06:22: #1 tags after filtering in treatment: 35736635 INFO @ Tue, 13 Aug 2019 00:06:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 00:06:22: #1 finished! INFO @ Tue, 13 Aug 2019 00:06:22: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 00:06:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 00:06:24: #2 number of paired peaks: 0 WARNING @ Tue, 13 Aug 2019 00:06:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 00:06:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 13 Aug 2019 00:06:25: #2 number of paired peaks: 0 WARNING @ Tue, 13 Aug 2019 00:06:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 00:06:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495793/SRX495793.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。