Job ID = 2590655 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 47,935,093 reads read : 47,935,093 reads written : 47,935,093 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:56 47935093 reads; of these: 47935093 (100.00%) were unpaired; of these: 4046989 (8.44%) aligned 0 times 36457504 (76.06%) aligned exactly 1 time 7430600 (15.50%) aligned >1 times 91.56% overall alignment rate Time searching: 00:12:56 Overall time: 00:12:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 16607621 / 43888104 = 0.3784 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 23:39:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:39:39: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:39:39: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:39:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:39:39: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:39:39: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:39:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:39:40: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:39:40: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:39:51: 1000000 INFO @ Mon, 12 Aug 2019 23:39:51: 1000000 INFO @ Mon, 12 Aug 2019 23:39:51: 1000000 INFO @ Mon, 12 Aug 2019 23:40:02: 2000000 INFO @ Mon, 12 Aug 2019 23:40:03: 2000000 INFO @ Mon, 12 Aug 2019 23:40:03: 2000000 INFO @ Mon, 12 Aug 2019 23:40:13: 3000000 INFO @ Mon, 12 Aug 2019 23:40:14: 3000000 INFO @ Mon, 12 Aug 2019 23:40:15: 3000000 INFO @ Mon, 12 Aug 2019 23:40:23: 4000000 INFO @ Mon, 12 Aug 2019 23:40:26: 4000000 INFO @ Mon, 12 Aug 2019 23:40:26: 4000000 INFO @ Mon, 12 Aug 2019 23:40:33: 5000000 INFO @ Mon, 12 Aug 2019 23:40:38: 5000000 INFO @ Mon, 12 Aug 2019 23:40:38: 5000000 INFO @ Mon, 12 Aug 2019 23:40:44: 6000000 INFO @ Mon, 12 Aug 2019 23:40:49: 6000000 INFO @ Mon, 12 Aug 2019 23:40:49: 6000000 INFO @ Mon, 12 Aug 2019 23:40:54: 7000000 INFO @ Mon, 12 Aug 2019 23:41:00: 7000000 INFO @ Mon, 12 Aug 2019 23:41:01: 7000000 INFO @ Mon, 12 Aug 2019 23:41:04: 8000000 INFO @ Mon, 12 Aug 2019 23:41:12: 8000000 INFO @ Mon, 12 Aug 2019 23:41:12: 8000000 INFO @ Mon, 12 Aug 2019 23:41:14: 9000000 INFO @ Mon, 12 Aug 2019 23:41:23: 9000000 INFO @ Mon, 12 Aug 2019 23:41:23: 9000000 INFO @ Mon, 12 Aug 2019 23:41:24: 10000000 INFO @ Mon, 12 Aug 2019 23:41:35: 10000000 INFO @ Mon, 12 Aug 2019 23:41:35: 11000000 INFO @ Mon, 12 Aug 2019 23:41:35: 10000000 INFO @ Mon, 12 Aug 2019 23:41:45: 12000000 INFO @ Mon, 12 Aug 2019 23:41:46: 11000000 INFO @ Mon, 12 Aug 2019 23:41:46: 11000000 INFO @ Mon, 12 Aug 2019 23:41:55: 13000000 INFO @ Mon, 12 Aug 2019 23:41:57: 12000000 INFO @ Mon, 12 Aug 2019 23:41:58: 12000000 INFO @ Mon, 12 Aug 2019 23:42:05: 14000000 INFO @ Mon, 12 Aug 2019 23:42:09: 13000000 INFO @ Mon, 12 Aug 2019 23:42:09: 13000000 INFO @ Mon, 12 Aug 2019 23:42:15: 15000000 INFO @ Mon, 12 Aug 2019 23:42:20: 14000000 INFO @ Mon, 12 Aug 2019 23:42:20: 14000000 INFO @ Mon, 12 Aug 2019 23:42:25: 16000000 INFO @ Mon, 12 Aug 2019 23:42:31: 15000000 INFO @ Mon, 12 Aug 2019 23:42:32: 15000000 INFO @ Mon, 12 Aug 2019 23:42:35: 17000000 INFO @ Mon, 12 Aug 2019 23:42:42: 16000000 INFO @ Mon, 12 Aug 2019 23:42:43: 16000000 INFO @ Mon, 12 Aug 2019 23:42:46: 18000000 INFO @ Mon, 12 Aug 2019 23:42:54: 17000000 INFO @ Mon, 12 Aug 2019 23:42:54: 17000000 INFO @ Mon, 12 Aug 2019 23:42:56: 19000000 INFO @ Mon, 12 Aug 2019 23:43:05: 18000000 INFO @ Mon, 12 Aug 2019 23:43:05: 18000000 INFO @ Mon, 12 Aug 2019 23:43:06: 20000000 INFO @ Mon, 12 Aug 2019 23:43:16: 19000000 INFO @ Mon, 12 Aug 2019 23:43:16: 21000000 INFO @ Mon, 12 Aug 2019 23:43:17: 19000000 INFO @ Mon, 12 Aug 2019 23:43:27: 22000000 INFO @ Mon, 12 Aug 2019 23:43:28: 20000000 INFO @ Mon, 12 Aug 2019 23:43:28: 20000000 INFO @ Mon, 12 Aug 2019 23:43:37: 23000000 INFO @ Mon, 12 Aug 2019 23:43:39: 21000000 INFO @ Mon, 12 Aug 2019 23:43:40: 21000000 INFO @ Mon, 12 Aug 2019 23:43:47: 24000000 INFO @ Mon, 12 Aug 2019 23:43:51: 22000000 INFO @ Mon, 12 Aug 2019 23:43:51: 22000000 INFO @ Mon, 12 Aug 2019 23:43:58: 25000000 INFO @ Mon, 12 Aug 2019 23:44:02: 23000000 INFO @ Mon, 12 Aug 2019 23:44:03: 23000000 INFO @ Mon, 12 Aug 2019 23:44:08: 26000000 INFO @ Mon, 12 Aug 2019 23:44:14: 24000000 INFO @ Mon, 12 Aug 2019 23:44:14: 24000000 INFO @ Mon, 12 Aug 2019 23:44:19: 27000000 INFO @ Mon, 12 Aug 2019 23:44:22: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:44:22: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:44:22: #1 total tags in treatment: 27280483 INFO @ Mon, 12 Aug 2019 23:44:22: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:44:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:44:22: #1 tags after filtering in treatment: 27280483 INFO @ Mon, 12 Aug 2019 23:44:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:44:22: #1 finished! INFO @ Mon, 12 Aug 2019 23:44:22: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:44:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:44:25: #2 number of paired peaks: 6 WARNING @ Mon, 12 Aug 2019 23:44:25: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:44:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:44:26: 25000000 INFO @ Mon, 12 Aug 2019 23:44:26: 25000000 INFO @ Mon, 12 Aug 2019 23:44:37: 26000000 INFO @ Mon, 12 Aug 2019 23:44:38: 26000000 INFO @ Mon, 12 Aug 2019 23:44:49: 27000000 INFO @ Mon, 12 Aug 2019 23:44:49: 27000000 INFO @ Mon, 12 Aug 2019 23:44:52: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:44:52: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:44:52: #1 total tags in treatment: 27280483 INFO @ Mon, 12 Aug 2019 23:44:52: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:44:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:44:52: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:44:52: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:44:52: #1 total tags in treatment: 27280483 INFO @ Mon, 12 Aug 2019 23:44:52: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:44:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:44:52: #1 tags after filtering in treatment: 27280483 INFO @ Mon, 12 Aug 2019 23:44:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:44:52: #1 finished! INFO @ Mon, 12 Aug 2019 23:44:52: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:44:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:44:53: #1 tags after filtering in treatment: 27280483 INFO @ Mon, 12 Aug 2019 23:44:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:44:53: #1 finished! INFO @ Mon, 12 Aug 2019 23:44:53: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:44:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:44:54: #2 number of paired peaks: 6 WARNING @ Mon, 12 Aug 2019 23:44:54: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:44:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:44:55: #2 number of paired peaks: 6 WARNING @ Mon, 12 Aug 2019 23:44:55: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:44:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495792/SRX495792.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。