Job ID = 1306421 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 33,349,222 reads read : 33,349,222 reads written : 33,349,222 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:16 33349222 reads; of these: 33349222 (100.00%) were unpaired; of these: 4936613 (14.80%) aligned 0 times 22866992 (68.57%) aligned exactly 1 time 5545617 (16.63%) aligned >1 times 85.20% overall alignment rate Time searching: 00:11:16 Overall time: 00:11:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5663324 / 28412609 = 0.1993 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 22:14:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:14:06: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:14:06: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:14:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:14:06: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:14:06: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:14:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:14:06: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:14:06: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:14:15: 1000000 INFO @ Mon, 03 Jun 2019 22:14:15: 1000000 INFO @ Mon, 03 Jun 2019 22:14:17: 1000000 INFO @ Mon, 03 Jun 2019 22:14:22: 2000000 INFO @ Mon, 03 Jun 2019 22:14:23: 2000000 INFO @ Mon, 03 Jun 2019 22:14:27: 2000000 INFO @ Mon, 03 Jun 2019 22:14:29: 3000000 INFO @ Mon, 03 Jun 2019 22:14:31: 3000000 INFO @ Mon, 03 Jun 2019 22:14:37: 4000000 INFO @ Mon, 03 Jun 2019 22:14:38: 3000000 INFO @ Mon, 03 Jun 2019 22:14:40: 4000000 INFO @ Mon, 03 Jun 2019 22:14:45: 5000000 INFO @ Mon, 03 Jun 2019 22:14:48: 5000000 INFO @ Mon, 03 Jun 2019 22:14:48: 4000000 INFO @ Mon, 03 Jun 2019 22:14:52: 6000000 INFO @ Mon, 03 Jun 2019 22:14:56: 6000000 INFO @ Mon, 03 Jun 2019 22:14:58: 5000000 INFO @ Mon, 03 Jun 2019 22:15:00: 7000000 INFO @ Mon, 03 Jun 2019 22:15:04: 7000000 INFO @ Mon, 03 Jun 2019 22:15:07: 8000000 INFO @ Mon, 03 Jun 2019 22:15:09: 6000000 INFO @ Mon, 03 Jun 2019 22:15:12: 8000000 INFO @ Mon, 03 Jun 2019 22:15:15: 9000000 INFO @ Mon, 03 Jun 2019 22:15:19: 7000000 INFO @ Mon, 03 Jun 2019 22:15:20: 9000000 INFO @ Mon, 03 Jun 2019 22:15:22: 10000000 INFO @ Mon, 03 Jun 2019 22:15:29: 10000000 INFO @ Mon, 03 Jun 2019 22:15:29: 8000000 INFO @ Mon, 03 Jun 2019 22:15:29: 11000000 INFO @ Mon, 03 Jun 2019 22:15:37: 11000000 INFO @ Mon, 03 Jun 2019 22:15:37: 12000000 INFO @ Mon, 03 Jun 2019 22:15:39: 9000000 INFO @ Mon, 03 Jun 2019 22:15:44: 13000000 INFO @ Mon, 03 Jun 2019 22:15:45: 12000000 INFO @ Mon, 03 Jun 2019 22:15:49: 10000000 INFO @ Mon, 03 Jun 2019 22:15:51: 14000000 INFO @ Mon, 03 Jun 2019 22:15:53: 13000000 INFO @ Mon, 03 Jun 2019 22:15:59: 15000000 INFO @ Mon, 03 Jun 2019 22:15:59: 11000000 INFO @ Mon, 03 Jun 2019 22:16:01: 14000000 INFO @ Mon, 03 Jun 2019 22:16:06: 16000000 INFO @ Mon, 03 Jun 2019 22:16:09: 15000000 INFO @ Mon, 03 Jun 2019 22:16:09: 12000000 INFO @ Mon, 03 Jun 2019 22:16:13: 17000000 INFO @ Mon, 03 Jun 2019 22:16:17: 16000000 INFO @ Mon, 03 Jun 2019 22:16:19: 13000000 INFO @ Mon, 03 Jun 2019 22:16:21: 18000000 INFO @ Mon, 03 Jun 2019 22:16:25: 17000000 INFO @ Mon, 03 Jun 2019 22:16:28: 19000000 INFO @ Mon, 03 Jun 2019 22:16:29: 14000000 INFO @ Mon, 03 Jun 2019 22:16:33: 18000000 INFO @ Mon, 03 Jun 2019 22:16:36: 20000000 INFO @ Mon, 03 Jun 2019 22:16:39: 15000000 INFO @ Mon, 03 Jun 2019 22:16:41: 19000000 INFO @ Mon, 03 Jun 2019 22:16:43: 21000000 INFO @ Mon, 03 Jun 2019 22:16:49: 20000000 INFO @ Mon, 03 Jun 2019 22:16:49: 16000000 INFO @ Mon, 03 Jun 2019 22:16:50: 22000000 INFO @ Mon, 03 Jun 2019 22:16:56: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:16:56: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:16:56: #1 total tags in treatment: 22749285 INFO @ Mon, 03 Jun 2019 22:16:56: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:16:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:16:56: #1 tags after filtering in treatment: 22749285 INFO @ Mon, 03 Jun 2019 22:16:56: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:16:56: #1 finished! INFO @ Mon, 03 Jun 2019 22:16:56: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:16:56: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:16:57: 21000000 INFO @ Mon, 03 Jun 2019 22:16:58: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 22:16:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:16:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 22:17:00: 17000000 INFO @ Mon, 03 Jun 2019 22:17:05: 22000000 INFO @ Mon, 03 Jun 2019 22:17:11: 18000000 INFO @ Mon, 03 Jun 2019 22:17:11: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:17:11: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:17:11: #1 total tags in treatment: 22749285 INFO @ Mon, 03 Jun 2019 22:17:11: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:17:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:17:12: #1 tags after filtering in treatment: 22749285 INFO @ Mon, 03 Jun 2019 22:17:12: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:17:12: #1 finished! INFO @ Mon, 03 Jun 2019 22:17:12: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:17:12: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:17:14: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 22:17:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:17:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 22:17:21: 19000000 INFO @ Mon, 03 Jun 2019 22:17:32: 20000000 INFO @ Mon, 03 Jun 2019 22:17:42: 21000000 INFO @ Mon, 03 Jun 2019 22:17:53: 22000000 INFO @ Mon, 03 Jun 2019 22:18:00: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:18:00: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:18:00: #1 total tags in treatment: 22749285 INFO @ Mon, 03 Jun 2019 22:18:00: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:18:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:18:01: #1 tags after filtering in treatment: 22749285 INFO @ Mon, 03 Jun 2019 22:18:01: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:18:01: #1 finished! INFO @ Mon, 03 Jun 2019 22:18:01: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:18:01: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:18:03: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 22:18:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:18:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495782/SRX495782.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。