Job ID = 2590646 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 31,886,596 reads read : 31,886,596 reads written : 31,886,596 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:24 31886596 reads; of these: 31886596 (100.00%) were unpaired; of these: 2849470 (8.94%) aligned 0 times 22878855 (71.75%) aligned exactly 1 time 6158271 (19.31%) aligned >1 times 91.06% overall alignment rate Time searching: 00:10:24 Overall time: 00:10:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7307163 / 29037126 = 0.2516 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 23:24:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:24:33: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:24:33: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:24:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:24:34: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:24:34: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:24:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:24:35: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:24:35: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:24:39: 1000000 INFO @ Mon, 12 Aug 2019 23:24:41: 1000000 INFO @ Mon, 12 Aug 2019 23:24:42: 1000000 INFO @ Mon, 12 Aug 2019 23:24:46: 2000000 INFO @ Mon, 12 Aug 2019 23:24:49: 2000000 INFO @ Mon, 12 Aug 2019 23:24:50: 2000000 INFO @ Mon, 12 Aug 2019 23:24:53: 3000000 INFO @ Mon, 12 Aug 2019 23:24:56: 3000000 INFO @ Mon, 12 Aug 2019 23:24:57: 3000000 INFO @ Mon, 12 Aug 2019 23:25:00: 4000000 INFO @ Mon, 12 Aug 2019 23:25:04: 4000000 INFO @ Mon, 12 Aug 2019 23:25:05: 4000000 INFO @ Mon, 12 Aug 2019 23:25:06: 5000000 INFO @ Mon, 12 Aug 2019 23:25:12: 5000000 INFO @ Mon, 12 Aug 2019 23:25:13: 5000000 INFO @ Mon, 12 Aug 2019 23:25:13: 6000000 INFO @ Mon, 12 Aug 2019 23:25:19: 6000000 INFO @ Mon, 12 Aug 2019 23:25:20: 7000000 INFO @ Mon, 12 Aug 2019 23:25:20: 6000000 INFO @ Mon, 12 Aug 2019 23:25:27: 8000000 INFO @ Mon, 12 Aug 2019 23:25:27: 7000000 INFO @ Mon, 12 Aug 2019 23:25:28: 7000000 INFO @ Mon, 12 Aug 2019 23:25:34: 9000000 INFO @ Mon, 12 Aug 2019 23:25:35: 8000000 INFO @ Mon, 12 Aug 2019 23:25:36: 8000000 INFO @ Mon, 12 Aug 2019 23:25:40: 10000000 INFO @ Mon, 12 Aug 2019 23:25:43: 9000000 INFO @ Mon, 12 Aug 2019 23:25:43: 9000000 INFO @ Mon, 12 Aug 2019 23:25:47: 11000000 INFO @ Mon, 12 Aug 2019 23:25:51: 10000000 INFO @ Mon, 12 Aug 2019 23:25:51: 10000000 INFO @ Mon, 12 Aug 2019 23:25:54: 12000000 INFO @ Mon, 12 Aug 2019 23:25:58: 11000000 INFO @ Mon, 12 Aug 2019 23:25:59: 11000000 INFO @ Mon, 12 Aug 2019 23:26:01: 13000000 INFO @ Mon, 12 Aug 2019 23:26:06: 12000000 INFO @ Mon, 12 Aug 2019 23:26:07: 12000000 INFO @ Mon, 12 Aug 2019 23:26:08: 14000000 INFO @ Mon, 12 Aug 2019 23:26:13: 13000000 INFO @ Mon, 12 Aug 2019 23:26:15: 15000000 INFO @ Mon, 12 Aug 2019 23:26:15: 13000000 INFO @ Mon, 12 Aug 2019 23:26:21: 14000000 INFO @ Mon, 12 Aug 2019 23:26:22: 16000000 INFO @ Mon, 12 Aug 2019 23:26:23: 14000000 INFO @ Mon, 12 Aug 2019 23:26:28: 17000000 INFO @ Mon, 12 Aug 2019 23:26:29: 15000000 INFO @ Mon, 12 Aug 2019 23:26:30: 15000000 INFO @ Mon, 12 Aug 2019 23:26:35: 18000000 INFO @ Mon, 12 Aug 2019 23:26:36: 16000000 INFO @ Mon, 12 Aug 2019 23:26:38: 16000000 INFO @ Mon, 12 Aug 2019 23:26:42: 19000000 INFO @ Mon, 12 Aug 2019 23:26:44: 17000000 INFO @ Mon, 12 Aug 2019 23:26:46: 17000000 INFO @ Mon, 12 Aug 2019 23:26:49: 20000000 INFO @ Mon, 12 Aug 2019 23:26:51: 18000000 INFO @ Mon, 12 Aug 2019 23:26:53: 18000000 INFO @ Mon, 12 Aug 2019 23:26:56: 21000000 INFO @ Mon, 12 Aug 2019 23:26:59: 19000000 INFO @ Mon, 12 Aug 2019 23:27:01: 19000000 INFO @ Mon, 12 Aug 2019 23:27:01: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:27:01: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:27:01: #1 total tags in treatment: 21729963 INFO @ Mon, 12 Aug 2019 23:27:01: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:27:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:27:02: #1 tags after filtering in treatment: 21729963 INFO @ Mon, 12 Aug 2019 23:27:02: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:27:02: #1 finished! INFO @ Mon, 12 Aug 2019 23:27:02: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:27:02: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:27:04: #2 number of paired peaks: 8 WARNING @ Mon, 12 Aug 2019 23:27:04: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:27:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:27:06: 20000000 INFO @ Mon, 12 Aug 2019 23:27:08: 20000000 INFO @ Mon, 12 Aug 2019 23:27:13: 21000000 INFO @ Mon, 12 Aug 2019 23:27:16: 21000000 INFO @ Mon, 12 Aug 2019 23:27:19: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:27:19: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:27:19: #1 total tags in treatment: 21729963 INFO @ Mon, 12 Aug 2019 23:27:19: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:27:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:27:19: #1 tags after filtering in treatment: 21729963 INFO @ Mon, 12 Aug 2019 23:27:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:27:19: #1 finished! INFO @ Mon, 12 Aug 2019 23:27:19: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:27:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:27:21: #2 number of paired peaks: 8 WARNING @ Mon, 12 Aug 2019 23:27:21: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:27:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.10_peaks.narrowPeak: No such file or directory INFO @ Mon, 12 Aug 2019 23:27:21: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:27:21: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:27:21: #1 total tags in treatment: 21729963 INFO @ Mon, 12 Aug 2019 23:27:21: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:27:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:27:22: #1 tags after filtering in treatment: 21729963 INFO @ Mon, 12 Aug 2019 23:27:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:27:22: #1 finished! INFO @ Mon, 12 Aug 2019 23:27:22: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:27:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:27:24: #2 number of paired peaks: 8 WARNING @ Mon, 12 Aug 2019 23:27:24: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:27:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495781/SRX495781.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。