Job ID = 2590645 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 32,203,362 reads read : 32,203,362 reads written : 32,203,362 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:45 32203362 reads; of these: 32203362 (100.00%) were unpaired; of these: 3172148 (9.85%) aligned 0 times 22508895 (69.90%) aligned exactly 1 time 6522319 (20.25%) aligned >1 times 90.15% overall alignment rate Time searching: 00:09:45 Overall time: 00:09:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7038009 / 29031214 = 0.2424 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 23:25:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:25:41: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:25:41: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:25:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:25:42: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:25:42: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:25:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:25:43: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:25:43: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:25:48: 1000000 INFO @ Mon, 12 Aug 2019 23:25:50: 1000000 INFO @ Mon, 12 Aug 2019 23:25:51: 1000000 INFO @ Mon, 12 Aug 2019 23:25:55: 2000000 INFO @ Mon, 12 Aug 2019 23:25:57: 2000000 INFO @ Mon, 12 Aug 2019 23:25:59: 2000000 INFO @ Mon, 12 Aug 2019 23:26:02: 3000000 INFO @ Mon, 12 Aug 2019 23:26:04: 3000000 INFO @ Mon, 12 Aug 2019 23:26:06: 3000000 INFO @ Mon, 12 Aug 2019 23:26:09: 4000000 INFO @ Mon, 12 Aug 2019 23:26:11: 4000000 INFO @ Mon, 12 Aug 2019 23:26:14: 4000000 INFO @ Mon, 12 Aug 2019 23:26:16: 5000000 INFO @ Mon, 12 Aug 2019 23:26:18: 5000000 INFO @ Mon, 12 Aug 2019 23:26:21: 5000000 INFO @ Mon, 12 Aug 2019 23:26:23: 6000000 INFO @ Mon, 12 Aug 2019 23:26:25: 6000000 INFO @ Mon, 12 Aug 2019 23:26:29: 6000000 INFO @ Mon, 12 Aug 2019 23:26:31: 7000000 INFO @ Mon, 12 Aug 2019 23:26:32: 7000000 INFO @ Mon, 12 Aug 2019 23:26:37: 7000000 INFO @ Mon, 12 Aug 2019 23:26:38: 8000000 INFO @ Mon, 12 Aug 2019 23:26:39: 8000000 INFO @ Mon, 12 Aug 2019 23:26:44: 8000000 INFO @ Mon, 12 Aug 2019 23:26:46: 9000000 INFO @ Mon, 12 Aug 2019 23:26:47: 9000000 INFO @ Mon, 12 Aug 2019 23:26:52: 9000000 INFO @ Mon, 12 Aug 2019 23:26:54: 10000000 INFO @ Mon, 12 Aug 2019 23:26:56: 10000000 INFO @ Mon, 12 Aug 2019 23:27:01: 10000000 INFO @ Mon, 12 Aug 2019 23:27:03: 11000000 INFO @ Mon, 12 Aug 2019 23:27:04: 11000000 INFO @ Mon, 12 Aug 2019 23:27:09: 11000000 INFO @ Mon, 12 Aug 2019 23:27:11: 12000000 INFO @ Mon, 12 Aug 2019 23:27:13: 12000000 INFO @ Mon, 12 Aug 2019 23:27:17: 12000000 INFO @ Mon, 12 Aug 2019 23:27:18: 13000000 INFO @ Mon, 12 Aug 2019 23:27:20: 13000000 INFO @ Mon, 12 Aug 2019 23:27:25: 13000000 INFO @ Mon, 12 Aug 2019 23:27:25: 14000000 INFO @ Mon, 12 Aug 2019 23:27:26: 14000000 INFO @ Mon, 12 Aug 2019 23:27:32: 15000000 INFO @ Mon, 12 Aug 2019 23:27:32: 14000000 INFO @ Mon, 12 Aug 2019 23:27:33: 15000000 INFO @ Mon, 12 Aug 2019 23:27:39: 16000000 INFO @ Mon, 12 Aug 2019 23:27:39: 15000000 INFO @ Mon, 12 Aug 2019 23:27:40: 16000000 INFO @ Mon, 12 Aug 2019 23:27:46: 17000000 INFO @ Mon, 12 Aug 2019 23:27:47: 16000000 INFO @ Mon, 12 Aug 2019 23:27:48: 17000000 INFO @ Mon, 12 Aug 2019 23:27:53: 18000000 INFO @ Mon, 12 Aug 2019 23:27:54: 17000000 INFO @ Mon, 12 Aug 2019 23:27:56: 18000000 INFO @ Mon, 12 Aug 2019 23:27:59: 19000000 INFO @ Mon, 12 Aug 2019 23:28:02: 18000000 INFO @ Mon, 12 Aug 2019 23:28:04: 19000000 INFO @ Mon, 12 Aug 2019 23:28:06: 20000000 INFO @ Mon, 12 Aug 2019 23:28:09: 19000000 INFO @ Mon, 12 Aug 2019 23:28:11: 20000000 INFO @ Mon, 12 Aug 2019 23:28:13: 21000000 INFO @ Mon, 12 Aug 2019 23:28:17: 20000000 INFO @ Mon, 12 Aug 2019 23:28:19: 21000000 INFO @ Mon, 12 Aug 2019 23:28:20: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:28:20: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:28:20: #1 total tags in treatment: 21993205 INFO @ Mon, 12 Aug 2019 23:28:20: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:28:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:28:21: #1 tags after filtering in treatment: 21993205 INFO @ Mon, 12 Aug 2019 23:28:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:28:21: #1 finished! INFO @ Mon, 12 Aug 2019 23:28:21: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:28:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:28:22: #2 number of paired peaks: 7 WARNING @ Mon, 12 Aug 2019 23:28:22: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:28:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:28:24: 21000000 INFO @ Mon, 12 Aug 2019 23:28:27: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:28:27: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:28:27: #1 total tags in treatment: 21993205 INFO @ Mon, 12 Aug 2019 23:28:27: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:28:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:28:27: #1 tags after filtering in treatment: 21993205 INFO @ Mon, 12 Aug 2019 23:28:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:28:27: #1 finished! INFO @ Mon, 12 Aug 2019 23:28:27: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:28:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:28:29: #2 number of paired peaks: 7 WARNING @ Mon, 12 Aug 2019 23:28:29: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:28:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:28:32: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:28:32: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:28:32: #1 total tags in treatment: 21993205 INFO @ Mon, 12 Aug 2019 23:28:32: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:28:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:28:32: #1 tags after filtering in treatment: 21993205 INFO @ Mon, 12 Aug 2019 23:28:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:28:32: #1 finished! INFO @ Mon, 12 Aug 2019 23:28:32: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:28:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:28:34: #2 number of paired peaks: 7 WARNING @ Mon, 12 Aug 2019 23:28:34: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:28:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495780/SRX495780.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。