Job ID = 1306202 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,927,623 reads read : 19,927,623 reads written : 19,927,623 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:23 19927623 reads; of these: 19927623 (100.00%) were unpaired; of these: 222050 (1.11%) aligned 0 times 14963396 (75.09%) aligned exactly 1 time 4742177 (23.80%) aligned >1 times 98.89% overall alignment rate Time searching: 00:07:23 Overall time: 00:07:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4261106 / 19705573 = 0.2162 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:56:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:56:06: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:56:06: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:56:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:56:06: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:56:06: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:56:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:56:06: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:56:06: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:56:14: 1000000 INFO @ Mon, 03 Jun 2019 21:56:16: 1000000 INFO @ Mon, 03 Jun 2019 21:56:17: 1000000 INFO @ Mon, 03 Jun 2019 21:56:22: 2000000 INFO @ Mon, 03 Jun 2019 21:56:25: 2000000 INFO @ Mon, 03 Jun 2019 21:56:26: 2000000 INFO @ Mon, 03 Jun 2019 21:56:30: 3000000 INFO @ Mon, 03 Jun 2019 21:56:35: 3000000 INFO @ Mon, 03 Jun 2019 21:56:36: 3000000 INFO @ Mon, 03 Jun 2019 21:56:38: 4000000 INFO @ Mon, 03 Jun 2019 21:56:44: 4000000 INFO @ Mon, 03 Jun 2019 21:56:45: 4000000 INFO @ Mon, 03 Jun 2019 21:56:46: 5000000 INFO @ Mon, 03 Jun 2019 21:56:53: 6000000 INFO @ Mon, 03 Jun 2019 21:56:54: 5000000 INFO @ Mon, 03 Jun 2019 21:56:55: 5000000 INFO @ Mon, 03 Jun 2019 21:57:01: 7000000 INFO @ Mon, 03 Jun 2019 21:57:03: 6000000 INFO @ Mon, 03 Jun 2019 21:57:04: 6000000 INFO @ Mon, 03 Jun 2019 21:57:09: 8000000 INFO @ Mon, 03 Jun 2019 21:57:12: 7000000 INFO @ Mon, 03 Jun 2019 21:57:13: 7000000 INFO @ Mon, 03 Jun 2019 21:57:16: 9000000 INFO @ Mon, 03 Jun 2019 21:57:21: 8000000 INFO @ Mon, 03 Jun 2019 21:57:23: 8000000 INFO @ Mon, 03 Jun 2019 21:57:24: 10000000 INFO @ Mon, 03 Jun 2019 21:57:31: 9000000 INFO @ Mon, 03 Jun 2019 21:57:32: 11000000 INFO @ Mon, 03 Jun 2019 21:57:33: 9000000 INFO @ Mon, 03 Jun 2019 21:57:39: 12000000 INFO @ Mon, 03 Jun 2019 21:57:40: 10000000 INFO @ Mon, 03 Jun 2019 21:57:42: 10000000 INFO @ Mon, 03 Jun 2019 21:57:47: 13000000 INFO @ Mon, 03 Jun 2019 21:57:49: 11000000 INFO @ Mon, 03 Jun 2019 21:57:52: 11000000 INFO @ Mon, 03 Jun 2019 21:57:55: 14000000 INFO @ Mon, 03 Jun 2019 21:57:58: 12000000 INFO @ Mon, 03 Jun 2019 21:58:01: 12000000 INFO @ Mon, 03 Jun 2019 21:58:03: 15000000 INFO @ Mon, 03 Jun 2019 21:58:06: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:58:06: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:58:06: #1 total tags in treatment: 15444467 INFO @ Mon, 03 Jun 2019 21:58:06: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:58:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:58:07: #1 tags after filtering in treatment: 15444467 INFO @ Mon, 03 Jun 2019 21:58:07: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:58:07: #1 finished! INFO @ Mon, 03 Jun 2019 21:58:07: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:58:07: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:58:07: 13000000 INFO @ Mon, 03 Jun 2019 21:58:08: #2 number of paired peaks: 45 WARNING @ Mon, 03 Jun 2019 21:58:08: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:58:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:58:11: 13000000 INFO @ Mon, 03 Jun 2019 21:58:16: 14000000 INFO @ Mon, 03 Jun 2019 21:58:20: 14000000 INFO @ Mon, 03 Jun 2019 21:58:25: 15000000 INFO @ Mon, 03 Jun 2019 21:58:29: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:58:29: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:58:29: #1 total tags in treatment: 15444467 INFO @ Mon, 03 Jun 2019 21:58:29: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:58:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:58:29: #1 tags after filtering in treatment: 15444467 INFO @ Mon, 03 Jun 2019 21:58:29: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:58:29: #1 finished! INFO @ Mon, 03 Jun 2019 21:58:29: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:58:29: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:58:29: 15000000 INFO @ Mon, 03 Jun 2019 21:58:30: #2 number of paired peaks: 45 WARNING @ Mon, 03 Jun 2019 21:58:30: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:58:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:58:33: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 21:58:33: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 21:58:33: #1 total tags in treatment: 15444467 INFO @ Mon, 03 Jun 2019 21:58:33: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:58:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:58:33: #1 tags after filtering in treatment: 15444467 INFO @ Mon, 03 Jun 2019 21:58:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:58:33: #1 finished! INFO @ Mon, 03 Jun 2019 21:58:33: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:58:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:58:35: #2 number of paired peaks: 45 WARNING @ Mon, 03 Jun 2019 21:58:35: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:58:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495776/SRX495776.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。