Job ID = 1306180 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 36,935,373 reads read : 36,935,373 reads written : 36,935,373 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:32 36935373 reads; of these: 36935373 (100.00%) were unpaired; of these: 3489203 (9.45%) aligned 0 times 23710226 (64.19%) aligned exactly 1 time 9735944 (26.36%) aligned >1 times 90.55% overall alignment rate Time searching: 00:15:32 Overall time: 00:15:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 8662816 / 33446170 = 0.2590 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 22:14:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:14:25: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:14:25: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:14:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:14:25: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:14:25: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:14:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:14:25: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:14:25: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:14:36: 1000000 INFO @ Mon, 03 Jun 2019 22:14:36: 1000000 INFO @ Mon, 03 Jun 2019 22:14:40: 1000000 INFO @ Mon, 03 Jun 2019 22:14:46: 2000000 INFO @ Mon, 03 Jun 2019 22:14:46: 2000000 INFO @ Mon, 03 Jun 2019 22:14:55: 2000000 INFO @ Mon, 03 Jun 2019 22:14:56: 3000000 INFO @ Mon, 03 Jun 2019 22:14:56: 3000000 INFO @ Mon, 03 Jun 2019 22:15:06: 4000000 INFO @ Mon, 03 Jun 2019 22:15:06: 4000000 INFO @ Mon, 03 Jun 2019 22:15:10: 3000000 INFO @ Mon, 03 Jun 2019 22:15:16: 5000000 INFO @ Mon, 03 Jun 2019 22:15:16: 5000000 INFO @ Mon, 03 Jun 2019 22:15:24: 4000000 INFO @ Mon, 03 Jun 2019 22:15:26: 6000000 INFO @ Mon, 03 Jun 2019 22:15:26: 6000000 INFO @ Mon, 03 Jun 2019 22:15:36: 7000000 INFO @ Mon, 03 Jun 2019 22:15:36: 7000000 INFO @ Mon, 03 Jun 2019 22:15:39: 5000000 INFO @ Mon, 03 Jun 2019 22:15:46: 8000000 INFO @ Mon, 03 Jun 2019 22:15:46: 8000000 INFO @ Mon, 03 Jun 2019 22:15:54: 6000000 INFO @ Mon, 03 Jun 2019 22:15:56: 9000000 INFO @ Mon, 03 Jun 2019 22:15:56: 9000000 INFO @ Mon, 03 Jun 2019 22:16:06: 10000000 INFO @ Mon, 03 Jun 2019 22:16:06: 10000000 INFO @ Mon, 03 Jun 2019 22:16:08: 7000000 INFO @ Mon, 03 Jun 2019 22:16:16: 11000000 INFO @ Mon, 03 Jun 2019 22:16:16: 11000000 INFO @ Mon, 03 Jun 2019 22:16:23: 8000000 INFO @ Mon, 03 Jun 2019 22:16:26: 12000000 INFO @ Mon, 03 Jun 2019 22:16:26: 12000000 INFO @ Mon, 03 Jun 2019 22:16:36: 13000000 INFO @ Mon, 03 Jun 2019 22:16:36: 13000000 INFO @ Mon, 03 Jun 2019 22:16:38: 9000000 INFO @ Mon, 03 Jun 2019 22:16:46: 14000000 INFO @ Mon, 03 Jun 2019 22:16:46: 14000000 INFO @ Mon, 03 Jun 2019 22:16:52: 10000000 INFO @ Mon, 03 Jun 2019 22:16:57: 15000000 INFO @ Mon, 03 Jun 2019 22:16:57: 15000000 INFO @ Mon, 03 Jun 2019 22:17:07: 11000000 INFO @ Mon, 03 Jun 2019 22:17:08: 16000000 INFO @ Mon, 03 Jun 2019 22:17:08: 16000000 INFO @ Mon, 03 Jun 2019 22:17:18: 17000000 INFO @ Mon, 03 Jun 2019 22:17:18: 17000000 INFO @ Mon, 03 Jun 2019 22:17:22: 12000000 INFO @ Mon, 03 Jun 2019 22:17:28: 18000000 INFO @ Mon, 03 Jun 2019 22:17:28: 18000000 INFO @ Mon, 03 Jun 2019 22:17:37: 13000000 INFO @ Mon, 03 Jun 2019 22:17:38: 19000000 INFO @ Mon, 03 Jun 2019 22:17:38: 19000000 INFO @ Mon, 03 Jun 2019 22:17:48: 20000000 INFO @ Mon, 03 Jun 2019 22:17:48: 20000000 INFO @ Mon, 03 Jun 2019 22:17:52: 14000000 INFO @ Mon, 03 Jun 2019 22:17:58: 21000000 INFO @ Mon, 03 Jun 2019 22:17:58: 21000000 INFO @ Mon, 03 Jun 2019 22:18:06: 15000000 INFO @ Mon, 03 Jun 2019 22:18:07: 22000000 INFO @ Mon, 03 Jun 2019 22:18:08: 22000000 INFO @ Mon, 03 Jun 2019 22:18:15: 23000000 INFO @ Mon, 03 Jun 2019 22:18:18: 23000000 INFO @ Mon, 03 Jun 2019 22:18:21: 16000000 INFO @ Mon, 03 Jun 2019 22:18:24: 24000000 INFO @ Mon, 03 Jun 2019 22:18:27: 24000000 INFO @ Mon, 03 Jun 2019 22:18:30: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:18:30: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:18:30: #1 total tags in treatment: 24783354 INFO @ Mon, 03 Jun 2019 22:18:30: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:18:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:18:31: #1 tags after filtering in treatment: 24783354 INFO @ Mon, 03 Jun 2019 22:18:31: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:18:31: #1 finished! INFO @ Mon, 03 Jun 2019 22:18:31: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:18:31: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:18:33: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 22:18:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:18:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 22:18:35: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:18:35: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:18:35: #1 total tags in treatment: 24783354 INFO @ Mon, 03 Jun 2019 22:18:35: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:18:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:18:35: #1 tags after filtering in treatment: 24783354 INFO @ Mon, 03 Jun 2019 22:18:35: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:18:35: #1 finished! INFO @ Mon, 03 Jun 2019 22:18:35: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:18:35: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:18:35: 17000000 INFO @ Mon, 03 Jun 2019 22:18:37: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 22:18:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:18:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 22:18:48: 18000000 INFO @ Mon, 03 Jun 2019 22:19:00: 19000000 INFO @ Mon, 03 Jun 2019 22:19:12: 20000000 INFO @ Mon, 03 Jun 2019 22:19:25: 21000000 INFO @ Mon, 03 Jun 2019 22:19:37: 22000000 INFO @ Mon, 03 Jun 2019 22:19:49: 23000000 INFO @ Mon, 03 Jun 2019 22:20:01: 24000000 INFO @ Mon, 03 Jun 2019 22:20:11: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:20:11: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:20:11: #1 total tags in treatment: 24783354 INFO @ Mon, 03 Jun 2019 22:20:11: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:20:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:20:11: #1 tags after filtering in treatment: 24783354 INFO @ Mon, 03 Jun 2019 22:20:11: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:20:11: #1 finished! INFO @ Mon, 03 Jun 2019 22:20:11: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:20:11: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:20:13: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 22:20:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:20:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495324/SRX495324.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。