Job ID = 1306120 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 32,482,612 reads read : 32,482,612 reads written : 32,482,612 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:19 32482612 reads; of these: 32482612 (100.00%) were unpaired; of these: 1726498 (5.32%) aligned 0 times 21221794 (65.33%) aligned exactly 1 time 9534320 (29.35%) aligned >1 times 94.68% overall alignment rate Time searching: 00:14:19 Overall time: 00:14:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 8814423 / 30756114 = 0.2866 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 22:11:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:11:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:11:03: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:11:03: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:11:03: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:11:03: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:11:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:11:03: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:11:03: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:11:10: 1000000 INFO @ Mon, 03 Jun 2019 22:11:11: 1000000 INFO @ Mon, 03 Jun 2019 22:11:11: 1000000 INFO @ Mon, 03 Jun 2019 22:11:18: 2000000 INFO @ Mon, 03 Jun 2019 22:11:20: 2000000 INFO @ Mon, 03 Jun 2019 22:11:20: 2000000 INFO @ Mon, 03 Jun 2019 22:11:25: 3000000 INFO @ Mon, 03 Jun 2019 22:11:29: 3000000 INFO @ Mon, 03 Jun 2019 22:11:29: 3000000 INFO @ Mon, 03 Jun 2019 22:11:32: 4000000 INFO @ Mon, 03 Jun 2019 22:11:37: 4000000 INFO @ Mon, 03 Jun 2019 22:11:37: 4000000 INFO @ Mon, 03 Jun 2019 22:11:39: 5000000 INFO @ Mon, 03 Jun 2019 22:11:45: 5000000 INFO @ Mon, 03 Jun 2019 22:11:45: 5000000 INFO @ Mon, 03 Jun 2019 22:11:47: 6000000 INFO @ Mon, 03 Jun 2019 22:11:54: 7000000 INFO @ Mon, 03 Jun 2019 22:11:54: 6000000 INFO @ Mon, 03 Jun 2019 22:11:54: 6000000 INFO @ Mon, 03 Jun 2019 22:12:01: 8000000 INFO @ Mon, 03 Jun 2019 22:12:02: 7000000 INFO @ Mon, 03 Jun 2019 22:12:02: 7000000 INFO @ Mon, 03 Jun 2019 22:12:08: 9000000 INFO @ Mon, 03 Jun 2019 22:12:11: 8000000 INFO @ Mon, 03 Jun 2019 22:12:11: 8000000 INFO @ Mon, 03 Jun 2019 22:12:16: 10000000 INFO @ Mon, 03 Jun 2019 22:12:19: 9000000 INFO @ Mon, 03 Jun 2019 22:12:19: 9000000 INFO @ Mon, 03 Jun 2019 22:12:23: 11000000 INFO @ Mon, 03 Jun 2019 22:12:28: 10000000 INFO @ Mon, 03 Jun 2019 22:12:28: 10000000 INFO @ Mon, 03 Jun 2019 22:12:30: 12000000 INFO @ Mon, 03 Jun 2019 22:12:36: 11000000 INFO @ Mon, 03 Jun 2019 22:12:36: 11000000 INFO @ Mon, 03 Jun 2019 22:12:37: 13000000 INFO @ Mon, 03 Jun 2019 22:12:45: 12000000 INFO @ Mon, 03 Jun 2019 22:12:45: 12000000 INFO @ Mon, 03 Jun 2019 22:12:45: 14000000 INFO @ Mon, 03 Jun 2019 22:12:52: 15000000 INFO @ Mon, 03 Jun 2019 22:12:53: 13000000 INFO @ Mon, 03 Jun 2019 22:12:53: 13000000 INFO @ Mon, 03 Jun 2019 22:12:59: 16000000 INFO @ Mon, 03 Jun 2019 22:13:02: 14000000 INFO @ Mon, 03 Jun 2019 22:13:02: 14000000 INFO @ Mon, 03 Jun 2019 22:13:06: 17000000 INFO @ Mon, 03 Jun 2019 22:13:10: 15000000 INFO @ Mon, 03 Jun 2019 22:13:10: 15000000 INFO @ Mon, 03 Jun 2019 22:13:14: 18000000 INFO @ Mon, 03 Jun 2019 22:13:18: 16000000 INFO @ Mon, 03 Jun 2019 22:13:18: 16000000 INFO @ Mon, 03 Jun 2019 22:13:21: 19000000 INFO @ Mon, 03 Jun 2019 22:13:27: 17000000 INFO @ Mon, 03 Jun 2019 22:13:27: 17000000 INFO @ Mon, 03 Jun 2019 22:13:28: 20000000 INFO @ Mon, 03 Jun 2019 22:13:35: 18000000 INFO @ Mon, 03 Jun 2019 22:13:35: 18000000 INFO @ Mon, 03 Jun 2019 22:13:35: 21000000 INFO @ Mon, 03 Jun 2019 22:13:43: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:13:43: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:13:43: #1 total tags in treatment: 21941691 INFO @ Mon, 03 Jun 2019 22:13:43: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:13:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:13:43: #1 tags after filtering in treatment: 21941691 INFO @ Mon, 03 Jun 2019 22:13:43: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:13:43: #1 finished! INFO @ Mon, 03 Jun 2019 22:13:43: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:13:43: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:13:44: 19000000 INFO @ Mon, 03 Jun 2019 22:13:44: 19000000 INFO @ Mon, 03 Jun 2019 22:13:45: #2 number of paired peaks: 46 WARNING @ Mon, 03 Jun 2019 22:13:45: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:13:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 22:13:53: 20000000 INFO @ Mon, 03 Jun 2019 22:13:53: 20000000 INFO @ Mon, 03 Jun 2019 22:14:02: 21000000 INFO @ Mon, 03 Jun 2019 22:14:02: 21000000 INFO @ Mon, 03 Jun 2019 22:14:10: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:14:10: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:14:10: #1 total tags in treatment: 21941691 INFO @ Mon, 03 Jun 2019 22:14:10: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:14:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:14:10: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:14:10: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:14:10: #1 total tags in treatment: 21941691 INFO @ Mon, 03 Jun 2019 22:14:10: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:14:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:14:11: #1 tags after filtering in treatment: 21941691 INFO @ Mon, 03 Jun 2019 22:14:11: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:14:11: #1 finished! INFO @ Mon, 03 Jun 2019 22:14:11: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:14:11: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:14:11: #1 tags after filtering in treatment: 21941691 INFO @ Mon, 03 Jun 2019 22:14:11: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:14:11: #1 finished! INFO @ Mon, 03 Jun 2019 22:14:11: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:14:11: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:14:13: #2 number of paired peaks: 46 WARNING @ Mon, 03 Jun 2019 22:14:13: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:14:13: Process for pairing-model is terminated! INFO @ Mon, 03 Jun 2019 22:14:13: #2 number of paired peaks: 46 WARNING @ Mon, 03 Jun 2019 22:14:13: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:14:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.20_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.20_model.r’: No such file or directoryrm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.20_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495322/SRX495322.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。