Job ID = 1305888 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 22,570,048 reads read : 22,570,048 reads written : 22,570,048 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:59 22570048 reads; of these: 22570048 (100.00%) were unpaired; of these: 4589313 (20.33%) aligned 0 times 15643525 (69.31%) aligned exactly 1 time 2337210 (10.36%) aligned >1 times 79.67% overall alignment rate Time searching: 00:05:59 Overall time: 00:05:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3637069 / 17980735 = 0.2023 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:37:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:37:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:37:14: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:37:14: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:37:14: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:37:14: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:37:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:37:14: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:37:14: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:37:22: 1000000 INFO @ Mon, 03 Jun 2019 21:37:22: 1000000 INFO @ Mon, 03 Jun 2019 21:37:25: 1000000 INFO @ Mon, 03 Jun 2019 21:37:30: 2000000 INFO @ Mon, 03 Jun 2019 21:37:30: 2000000 INFO @ Mon, 03 Jun 2019 21:37:37: 2000000 INFO @ Mon, 03 Jun 2019 21:37:37: 3000000 INFO @ Mon, 03 Jun 2019 21:37:38: 3000000 INFO @ Mon, 03 Jun 2019 21:37:45: 4000000 INFO @ Mon, 03 Jun 2019 21:37:46: 4000000 INFO @ Mon, 03 Jun 2019 21:37:47: 3000000 INFO @ Mon, 03 Jun 2019 21:37:53: 5000000 INFO @ Mon, 03 Jun 2019 21:37:54: 5000000 INFO @ Mon, 03 Jun 2019 21:37:59: 4000000 INFO @ Mon, 03 Jun 2019 21:38:01: 6000000 INFO @ Mon, 03 Jun 2019 21:38:02: 6000000 INFO @ Mon, 03 Jun 2019 21:38:08: 7000000 INFO @ Mon, 03 Jun 2019 21:38:09: 7000000 INFO @ Mon, 03 Jun 2019 21:38:10: 5000000 INFO @ Mon, 03 Jun 2019 21:38:16: 8000000 INFO @ Mon, 03 Jun 2019 21:38:17: 8000000 INFO @ Mon, 03 Jun 2019 21:38:21: 6000000 INFO @ Mon, 03 Jun 2019 21:38:24: 9000000 INFO @ Mon, 03 Jun 2019 21:38:25: 9000000 INFO @ Mon, 03 Jun 2019 21:38:32: 10000000 INFO @ Mon, 03 Jun 2019 21:38:32: 7000000 INFO @ Mon, 03 Jun 2019 21:38:33: 10000000 INFO @ Mon, 03 Jun 2019 21:38:40: 11000000 INFO @ Mon, 03 Jun 2019 21:38:41: 11000000 INFO @ Mon, 03 Jun 2019 21:38:43: 8000000 INFO @ Mon, 03 Jun 2019 21:38:48: 12000000 INFO @ Mon, 03 Jun 2019 21:38:48: 12000000 INFO @ Mon, 03 Jun 2019 21:38:54: 9000000 INFO @ Mon, 03 Jun 2019 21:38:55: 13000000 INFO @ Mon, 03 Jun 2019 21:38:56: 13000000 INFO @ Mon, 03 Jun 2019 21:39:03: 14000000 INFO @ Mon, 03 Jun 2019 21:39:04: 14000000 INFO @ Mon, 03 Jun 2019 21:39:05: 10000000 INFO @ Mon, 03 Jun 2019 21:39:06: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:39:06: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:39:06: #1 total tags in treatment: 14343666 INFO @ Mon, 03 Jun 2019 21:39:06: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:39:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:39:06: #1 tags after filtering in treatment: 14343666 INFO @ Mon, 03 Jun 2019 21:39:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:39:06: #1 finished! INFO @ Mon, 03 Jun 2019 21:39:06: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:39:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:39:07: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:39:07: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:39:07: #1 total tags in treatment: 14343666 INFO @ Mon, 03 Jun 2019 21:39:07: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:39:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:39:07: #1 tags after filtering in treatment: 14343666 INFO @ Mon, 03 Jun 2019 21:39:07: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:39:07: #1 finished! INFO @ Mon, 03 Jun 2019 21:39:07: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:39:07: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:39:08: #2 number of paired peaks: 99 WARNING @ Mon, 03 Jun 2019 21:39:08: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:39:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:39:08: #2 number of paired peaks: 99 WARNING @ Mon, 03 Jun 2019 21:39:08: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:39:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:39:17: 11000000 INFO @ Mon, 03 Jun 2019 21:39:29: 12000000 INFO @ Mon, 03 Jun 2019 21:39:39: 13000000 INFO @ Mon, 03 Jun 2019 21:39:49: 14000000 INFO @ Mon, 03 Jun 2019 21:39:52: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:39:52: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:39:52: #1 total tags in treatment: 14343666 INFO @ Mon, 03 Jun 2019 21:39:52: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:39:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:39:53: #1 tags after filtering in treatment: 14343666 INFO @ Mon, 03 Jun 2019 21:39:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:39:53: #1 finished! INFO @ Mon, 03 Jun 2019 21:39:53: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:39:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:39:54: #2 number of paired peaks: 99 WARNING @ Mon, 03 Jun 2019 21:39:54: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:39:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495307/SRX495307.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。