Job ID = 1305845 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 35,836,077 reads read : 35,836,077 reads written : 35,836,077 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:33 35836077 reads; of these: 35836077 (100.00%) were unpaired; of these: 3658857 (10.21%) aligned 0 times 27705612 (77.31%) aligned exactly 1 time 4471608 (12.48%) aligned >1 times 89.79% overall alignment rate Time searching: 00:09:33 Overall time: 00:09:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 8368709 / 32177220 = 0.2601 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:45:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:45:17: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:45:17: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:45:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:45:17: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:45:17: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:45:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:45:17: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:45:17: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:45:24: 1000000 INFO @ Mon, 03 Jun 2019 21:45:26: 1000000 INFO @ Mon, 03 Jun 2019 21:45:27: 1000000 INFO @ Mon, 03 Jun 2019 21:45:31: 2000000 INFO @ Mon, 03 Jun 2019 21:45:35: 2000000 INFO @ Mon, 03 Jun 2019 21:45:36: 2000000 INFO @ Mon, 03 Jun 2019 21:45:38: 3000000 INFO @ Mon, 03 Jun 2019 21:45:43: 3000000 INFO @ Mon, 03 Jun 2019 21:45:44: 3000000 INFO @ Mon, 03 Jun 2019 21:45:45: 4000000 INFO @ Mon, 03 Jun 2019 21:45:51: 4000000 INFO @ Mon, 03 Jun 2019 21:45:51: 5000000 INFO @ Mon, 03 Jun 2019 21:45:52: 4000000 INFO @ Mon, 03 Jun 2019 21:45:58: 6000000 INFO @ Mon, 03 Jun 2019 21:45:59: 5000000 INFO @ Mon, 03 Jun 2019 21:46:01: 5000000 INFO @ Mon, 03 Jun 2019 21:46:05: 7000000 INFO @ Mon, 03 Jun 2019 21:46:07: 6000000 INFO @ Mon, 03 Jun 2019 21:46:09: 6000000 INFO @ Mon, 03 Jun 2019 21:46:12: 8000000 INFO @ Mon, 03 Jun 2019 21:46:15: 7000000 INFO @ Mon, 03 Jun 2019 21:46:17: 7000000 INFO @ Mon, 03 Jun 2019 21:46:19: 9000000 INFO @ Mon, 03 Jun 2019 21:46:24: 8000000 INFO @ Mon, 03 Jun 2019 21:46:25: 10000000 INFO @ Mon, 03 Jun 2019 21:46:26: 8000000 INFO @ Mon, 03 Jun 2019 21:46:32: 9000000 INFO @ Mon, 03 Jun 2019 21:46:32: 11000000 INFO @ Mon, 03 Jun 2019 21:46:35: 9000000 INFO @ Mon, 03 Jun 2019 21:46:39: 12000000 INFO @ Mon, 03 Jun 2019 21:46:40: 10000000 INFO @ Mon, 03 Jun 2019 21:46:43: 10000000 INFO @ Mon, 03 Jun 2019 21:46:46: 13000000 INFO @ Mon, 03 Jun 2019 21:46:48: 11000000 INFO @ Mon, 03 Jun 2019 21:46:51: 11000000 INFO @ Mon, 03 Jun 2019 21:46:53: 14000000 INFO @ Mon, 03 Jun 2019 21:46:56: 12000000 INFO @ Mon, 03 Jun 2019 21:47:00: 12000000 INFO @ Mon, 03 Jun 2019 21:47:00: 15000000 INFO @ Mon, 03 Jun 2019 21:47:04: 13000000 INFO @ Mon, 03 Jun 2019 21:47:07: 16000000 INFO @ Mon, 03 Jun 2019 21:47:08: 13000000 INFO @ Mon, 03 Jun 2019 21:47:13: 14000000 INFO @ Mon, 03 Jun 2019 21:47:14: 17000000 INFO @ Mon, 03 Jun 2019 21:47:17: 14000000 INFO @ Mon, 03 Jun 2019 21:47:20: 18000000 INFO @ Mon, 03 Jun 2019 21:47:21: 15000000 INFO @ Mon, 03 Jun 2019 21:47:25: 15000000 INFO @ Mon, 03 Jun 2019 21:47:27: 19000000 INFO @ Mon, 03 Jun 2019 21:47:30: 16000000 INFO @ Mon, 03 Jun 2019 21:47:33: 16000000 INFO @ Mon, 03 Jun 2019 21:47:34: 20000000 INFO @ Mon, 03 Jun 2019 21:47:38: 17000000 INFO @ Mon, 03 Jun 2019 21:47:40: 21000000 INFO @ Mon, 03 Jun 2019 21:47:42: 17000000 INFO @ Mon, 03 Jun 2019 21:47:46: 18000000 INFO @ Mon, 03 Jun 2019 21:47:47: 22000000 INFO @ Mon, 03 Jun 2019 21:47:51: 18000000 INFO @ Mon, 03 Jun 2019 21:47:54: 23000000 INFO @ Mon, 03 Jun 2019 21:47:54: 19000000 INFO @ Mon, 03 Jun 2019 21:47:59: 19000000 INFO @ Mon, 03 Jun 2019 21:47:59: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:47:59: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:47:59: #1 total tags in treatment: 23808511 INFO @ Mon, 03 Jun 2019 21:47:59: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:47:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:48:00: #1 tags after filtering in treatment: 23808511 INFO @ Mon, 03 Jun 2019 21:48:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:48:00: #1 finished! INFO @ Mon, 03 Jun 2019 21:48:00: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:48:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:48:02: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:48:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:48:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:48:03: 20000000 INFO @ Mon, 03 Jun 2019 21:48:07: 20000000 INFO @ Mon, 03 Jun 2019 21:48:11: 21000000 INFO @ Mon, 03 Jun 2019 21:48:16: 21000000 INFO @ Mon, 03 Jun 2019 21:48:19: 22000000 INFO @ Mon, 03 Jun 2019 21:48:24: 22000000 INFO @ Mon, 03 Jun 2019 21:48:27: 23000000 INFO @ Mon, 03 Jun 2019 21:48:33: 23000000 INFO @ Mon, 03 Jun 2019 21:48:33: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:48:33: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:48:33: #1 total tags in treatment: 23808511 INFO @ Mon, 03 Jun 2019 21:48:33: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:48:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:48:34: #1 tags after filtering in treatment: 23808511 INFO @ Mon, 03 Jun 2019 21:48:34: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:48:34: #1 finished! INFO @ Mon, 03 Jun 2019 21:48:34: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:48:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:48:36: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:48:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:48:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:48:40: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:48:40: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:48:40: #1 total tags in treatment: 23808511 INFO @ Mon, 03 Jun 2019 21:48:40: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:48:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:48:40: #1 tags after filtering in treatment: 23808511 INFO @ Mon, 03 Jun 2019 21:48:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:48:40: #1 finished! INFO @ Mon, 03 Jun 2019 21:48:40: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:48:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:48:42: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:48:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:48:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495304/SRX495304.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。