Job ID = 1305799 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 32,960,289 reads read : 32,960,289 reads written : 32,960,289 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:49 32960289 reads; of these: 32960289 (100.00%) were unpaired; of these: 4865837 (14.76%) aligned 0 times 22671953 (68.79%) aligned exactly 1 time 5422499 (16.45%) aligned >1 times 85.24% overall alignment rate Time searching: 00:10:49 Overall time: 00:10:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6368545 / 28094452 = 0.2267 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:45:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:45:08: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:45:08: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:45:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:45:08: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:45:08: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:45:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:45:08: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:45:08: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:45:16: 1000000 INFO @ Mon, 03 Jun 2019 21:45:16: 1000000 INFO @ Mon, 03 Jun 2019 21:45:17: 1000000 INFO @ Mon, 03 Jun 2019 21:45:24: 2000000 INFO @ Mon, 03 Jun 2019 21:45:24: 2000000 INFO @ Mon, 03 Jun 2019 21:45:27: 2000000 INFO @ Mon, 03 Jun 2019 21:45:31: 3000000 INFO @ Mon, 03 Jun 2019 21:45:32: 3000000 INFO @ Mon, 03 Jun 2019 21:45:36: 3000000 INFO @ Mon, 03 Jun 2019 21:45:39: 4000000 INFO @ Mon, 03 Jun 2019 21:45:40: 4000000 INFO @ Mon, 03 Jun 2019 21:45:45: 4000000 INFO @ Mon, 03 Jun 2019 21:45:47: 5000000 INFO @ Mon, 03 Jun 2019 21:45:47: 5000000 INFO @ Mon, 03 Jun 2019 21:45:54: 5000000 INFO @ Mon, 03 Jun 2019 21:45:54: 6000000 INFO @ Mon, 03 Jun 2019 21:45:55: 6000000 INFO @ Mon, 03 Jun 2019 21:46:02: 7000000 INFO @ Mon, 03 Jun 2019 21:46:03: 6000000 INFO @ Mon, 03 Jun 2019 21:46:03: 7000000 INFO @ Mon, 03 Jun 2019 21:46:10: 8000000 INFO @ Mon, 03 Jun 2019 21:46:11: 8000000 INFO @ Mon, 03 Jun 2019 21:46:12: 7000000 INFO @ Mon, 03 Jun 2019 21:46:17: 9000000 INFO @ Mon, 03 Jun 2019 21:46:19: 9000000 INFO @ Mon, 03 Jun 2019 21:46:20: 8000000 INFO @ Mon, 03 Jun 2019 21:46:25: 10000000 INFO @ Mon, 03 Jun 2019 21:46:26: 10000000 INFO @ Mon, 03 Jun 2019 21:46:29: 9000000 INFO @ Mon, 03 Jun 2019 21:46:33: 11000000 INFO @ Mon, 03 Jun 2019 21:46:34: 11000000 INFO @ Mon, 03 Jun 2019 21:46:38: 10000000 INFO @ Mon, 03 Jun 2019 21:46:40: 12000000 INFO @ Mon, 03 Jun 2019 21:46:42: 12000000 INFO @ Mon, 03 Jun 2019 21:46:48: 13000000 INFO @ Mon, 03 Jun 2019 21:46:48: 11000000 INFO @ Mon, 03 Jun 2019 21:46:50: 13000000 INFO @ Mon, 03 Jun 2019 21:46:55: 14000000 INFO @ Mon, 03 Jun 2019 21:46:56: 12000000 INFO @ Mon, 03 Jun 2019 21:46:58: 14000000 INFO @ Mon, 03 Jun 2019 21:47:03: 15000000 INFO @ Mon, 03 Jun 2019 21:47:05: 13000000 INFO @ Mon, 03 Jun 2019 21:47:05: 15000000 INFO @ Mon, 03 Jun 2019 21:47:11: 16000000 INFO @ Mon, 03 Jun 2019 21:47:13: 16000000 INFO @ Mon, 03 Jun 2019 21:47:14: 14000000 INFO @ Mon, 03 Jun 2019 21:47:18: 17000000 INFO @ Mon, 03 Jun 2019 21:47:21: 17000000 INFO @ Mon, 03 Jun 2019 21:47:23: 15000000 INFO @ Mon, 03 Jun 2019 21:47:26: 18000000 INFO @ Mon, 03 Jun 2019 21:47:29: 18000000 INFO @ Mon, 03 Jun 2019 21:47:32: 16000000 INFO @ Mon, 03 Jun 2019 21:47:34: 19000000 INFO @ Mon, 03 Jun 2019 21:47:37: 19000000 INFO @ Mon, 03 Jun 2019 21:47:40: 17000000 INFO @ Mon, 03 Jun 2019 21:47:42: 20000000 INFO @ Mon, 03 Jun 2019 21:47:45: 20000000 INFO @ Mon, 03 Jun 2019 21:47:49: 18000000 INFO @ Mon, 03 Jun 2019 21:47:49: 21000000 INFO @ Mon, 03 Jun 2019 21:47:53: 21000000 INFO @ Mon, 03 Jun 2019 21:47:55: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:47:55: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:47:55: #1 total tags in treatment: 21725907 INFO @ Mon, 03 Jun 2019 21:47:55: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:47:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:47:55: #1 tags after filtering in treatment: 21725907 INFO @ Mon, 03 Jun 2019 21:47:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:47:55: #1 finished! INFO @ Mon, 03 Jun 2019 21:47:55: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:47:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:47:57: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:47:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:47:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:47:58: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:47:58: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:47:58: #1 total tags in treatment: 21725907 INFO @ Mon, 03 Jun 2019 21:47:58: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:47:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:47:59: #1 tags after filtering in treatment: 21725907 INFO @ Mon, 03 Jun 2019 21:47:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:47:59: #1 finished! INFO @ Mon, 03 Jun 2019 21:47:59: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:47:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:47:59: 19000000 INFO @ Mon, 03 Jun 2019 21:48:01: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:48:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:48:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:48:08: 20000000 INFO @ Mon, 03 Jun 2019 21:48:17: 21000000 INFO @ Mon, 03 Jun 2019 21:48:23: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:48:23: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:48:23: #1 total tags in treatment: 21725907 INFO @ Mon, 03 Jun 2019 21:48:23: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:48:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:48:24: #1 tags after filtering in treatment: 21725907 INFO @ Mon, 03 Jun 2019 21:48:24: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:48:24: #1 finished! INFO @ Mon, 03 Jun 2019 21:48:24: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:48:24: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:48:25: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:48:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:48:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495303/SRX495303.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。