Job ID = 1305794 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 41,603,816 reads read : 41,603,816 reads written : 41,603,816 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:07 41603816 reads; of these: 41603816 (100.00%) were unpaired; of these: 5367042 (12.90%) aligned 0 times 30507915 (73.33%) aligned exactly 1 time 5728859 (13.77%) aligned >1 times 87.10% overall alignment rate Time searching: 00:14:07 Overall time: 00:14:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 9430460 / 36236774 = 0.2602 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:51:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:51:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:51:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:51:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:51:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:51:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:51:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:51:15: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:51:15: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:51:23: 1000000 INFO @ Mon, 03 Jun 2019 21:51:24: 1000000 INFO @ Mon, 03 Jun 2019 21:51:25: 1000000 INFO @ Mon, 03 Jun 2019 21:51:31: 2000000 INFO @ Mon, 03 Jun 2019 21:51:33: 2000000 INFO @ Mon, 03 Jun 2019 21:51:34: 2000000 INFO @ Mon, 03 Jun 2019 21:51:39: 3000000 INFO @ Mon, 03 Jun 2019 21:51:42: 3000000 INFO @ Mon, 03 Jun 2019 21:51:43: 3000000 INFO @ Mon, 03 Jun 2019 21:51:47: 4000000 INFO @ Mon, 03 Jun 2019 21:51:51: 4000000 INFO @ Mon, 03 Jun 2019 21:51:52: 4000000 INFO @ Mon, 03 Jun 2019 21:51:54: 5000000 INFO @ Mon, 03 Jun 2019 21:51:59: 5000000 INFO @ Mon, 03 Jun 2019 21:52:01: 5000000 INFO @ Mon, 03 Jun 2019 21:52:02: 6000000 INFO @ Mon, 03 Jun 2019 21:52:08: 6000000 INFO @ Mon, 03 Jun 2019 21:52:10: 7000000 INFO @ Mon, 03 Jun 2019 21:52:10: 6000000 INFO @ Mon, 03 Jun 2019 21:52:17: 7000000 INFO @ Mon, 03 Jun 2019 21:52:18: 8000000 INFO @ Mon, 03 Jun 2019 21:52:19: 7000000 INFO @ Mon, 03 Jun 2019 21:52:26: 9000000 INFO @ Mon, 03 Jun 2019 21:52:26: 8000000 INFO @ Mon, 03 Jun 2019 21:52:29: 8000000 INFO @ Mon, 03 Jun 2019 21:52:33: 10000000 INFO @ Mon, 03 Jun 2019 21:52:35: 9000000 INFO @ Mon, 03 Jun 2019 21:52:38: 9000000 INFO @ Mon, 03 Jun 2019 21:52:41: 11000000 INFO @ Mon, 03 Jun 2019 21:52:43: 10000000 INFO @ Mon, 03 Jun 2019 21:52:48: 10000000 INFO @ Mon, 03 Jun 2019 21:52:49: 12000000 INFO @ Mon, 03 Jun 2019 21:52:52: 11000000 INFO @ Mon, 03 Jun 2019 21:52:57: 11000000 INFO @ Mon, 03 Jun 2019 21:52:57: 13000000 INFO @ Mon, 03 Jun 2019 21:53:01: 12000000 INFO @ Mon, 03 Jun 2019 21:53:05: 14000000 INFO @ Mon, 03 Jun 2019 21:53:06: 12000000 INFO @ Mon, 03 Jun 2019 21:53:10: 13000000 INFO @ Mon, 03 Jun 2019 21:53:12: 15000000 INFO @ Mon, 03 Jun 2019 21:53:16: 13000000 INFO @ Mon, 03 Jun 2019 21:53:19: 14000000 INFO @ Mon, 03 Jun 2019 21:53:20: 16000000 INFO @ Mon, 03 Jun 2019 21:53:24: 14000000 INFO @ Mon, 03 Jun 2019 21:53:28: 17000000 INFO @ Mon, 03 Jun 2019 21:53:28: 15000000 INFO @ Mon, 03 Jun 2019 21:53:33: 15000000 INFO @ Mon, 03 Jun 2019 21:53:36: 18000000 INFO @ Mon, 03 Jun 2019 21:53:37: 16000000 INFO @ Mon, 03 Jun 2019 21:53:41: 16000000 INFO @ Mon, 03 Jun 2019 21:53:44: 19000000 INFO @ Mon, 03 Jun 2019 21:53:45: 17000000 INFO @ Mon, 03 Jun 2019 21:53:50: 17000000 INFO @ Mon, 03 Jun 2019 21:53:51: 20000000 INFO @ Mon, 03 Jun 2019 21:53:55: 18000000 INFO @ Mon, 03 Jun 2019 21:53:59: 21000000 INFO @ Mon, 03 Jun 2019 21:54:00: 18000000 INFO @ Mon, 03 Jun 2019 21:54:04: 19000000 INFO @ Mon, 03 Jun 2019 21:54:07: 22000000 INFO @ Mon, 03 Jun 2019 21:54:09: 19000000 INFO @ Mon, 03 Jun 2019 21:54:12: 20000000 INFO @ Mon, 03 Jun 2019 21:54:15: 23000000 INFO @ Mon, 03 Jun 2019 21:54:18: 20000000 INFO @ Mon, 03 Jun 2019 21:54:21: 21000000 INFO @ Mon, 03 Jun 2019 21:54:22: 24000000 INFO @ Mon, 03 Jun 2019 21:54:27: 21000000 INFO @ Mon, 03 Jun 2019 21:54:30: 25000000 INFO @ Mon, 03 Jun 2019 21:54:31: 22000000 INFO @ Mon, 03 Jun 2019 21:54:36: 22000000 INFO @ Mon, 03 Jun 2019 21:54:38: 26000000 INFO @ Mon, 03 Jun 2019 21:54:40: 23000000 INFO @ Mon, 03 Jun 2019 21:54:44: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:54:44: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:54:44: #1 total tags in treatment: 26806314 INFO @ Mon, 03 Jun 2019 21:54:44: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:54:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:54:45: #1 tags after filtering in treatment: 26806314 INFO @ Mon, 03 Jun 2019 21:54:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:54:45: #1 finished! INFO @ Mon, 03 Jun 2019 21:54:45: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:54:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:54:45: 23000000 INFO @ Mon, 03 Jun 2019 21:54:47: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:54:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:54:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:54:48: 24000000 INFO @ Mon, 03 Jun 2019 21:54:54: 24000000 INFO @ Mon, 03 Jun 2019 21:54:57: 25000000 INFO @ Mon, 03 Jun 2019 21:55:03: 25000000 INFO @ Mon, 03 Jun 2019 21:55:05: 26000000 INFO @ Mon, 03 Jun 2019 21:55:11: 26000000 INFO @ Mon, 03 Jun 2019 21:55:12: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:55:12: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:55:12: #1 total tags in treatment: 26806314 INFO @ Mon, 03 Jun 2019 21:55:12: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:55:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:55:13: #1 tags after filtering in treatment: 26806314 INFO @ Mon, 03 Jun 2019 21:55:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:55:13: #1 finished! INFO @ Mon, 03 Jun 2019 21:55:13: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:55:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:55:15: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:55:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:55:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:55:19: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:55:19: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:55:19: #1 total tags in treatment: 26806314 INFO @ Mon, 03 Jun 2019 21:55:19: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:55:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:55:19: #1 tags after filtering in treatment: 26806314 INFO @ Mon, 03 Jun 2019 21:55:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:55:19: #1 finished! INFO @ Mon, 03 Jun 2019 21:55:19: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:55:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:55:21: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:55:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:55:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495302/SRX495302.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。