Job ID = 1305747 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 31,586,842 reads read : 31,586,842 reads written : 31,586,842 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:29 31586842 reads; of these: 31586842 (100.00%) were unpaired; of these: 1435526 (4.54%) aligned 0 times 24318064 (76.99%) aligned exactly 1 time 5833252 (18.47%) aligned >1 times 95.46% overall alignment rate Time searching: 00:10:29 Overall time: 00:10:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6854578 / 30151316 = 0.2273 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:44:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:44:35: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:44:35: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:44:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:44:35: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:44:35: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:44:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:44:35: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:44:35: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:44:44: 1000000 INFO @ Mon, 03 Jun 2019 21:44:44: 1000000 INFO @ Mon, 03 Jun 2019 21:44:45: 1000000 INFO @ Mon, 03 Jun 2019 21:44:53: 2000000 INFO @ Mon, 03 Jun 2019 21:44:53: 2000000 INFO @ Mon, 03 Jun 2019 21:44:55: 2000000 INFO @ Mon, 03 Jun 2019 21:45:02: 3000000 INFO @ Mon, 03 Jun 2019 21:45:02: 3000000 INFO @ Mon, 03 Jun 2019 21:45:06: 3000000 INFO @ Mon, 03 Jun 2019 21:45:11: 4000000 INFO @ Mon, 03 Jun 2019 21:45:11: 4000000 INFO @ Mon, 03 Jun 2019 21:45:15: 4000000 INFO @ Mon, 03 Jun 2019 21:45:20: 5000000 INFO @ Mon, 03 Jun 2019 21:45:20: 5000000 INFO @ Mon, 03 Jun 2019 21:45:25: 5000000 INFO @ Mon, 03 Jun 2019 21:45:29: 6000000 INFO @ Mon, 03 Jun 2019 21:45:29: 6000000 INFO @ Mon, 03 Jun 2019 21:45:36: 6000000 INFO @ Mon, 03 Jun 2019 21:45:37: 7000000 INFO @ Mon, 03 Jun 2019 21:45:37: 7000000 INFO @ Mon, 03 Jun 2019 21:45:46: 8000000 INFO @ Mon, 03 Jun 2019 21:45:46: 8000000 INFO @ Mon, 03 Jun 2019 21:45:46: 7000000 INFO @ Mon, 03 Jun 2019 21:45:54: 9000000 INFO @ Mon, 03 Jun 2019 21:45:54: 9000000 INFO @ Mon, 03 Jun 2019 21:45:56: 8000000 INFO @ Mon, 03 Jun 2019 21:46:03: 10000000 INFO @ Mon, 03 Jun 2019 21:46:03: 10000000 INFO @ Mon, 03 Jun 2019 21:46:06: 9000000 INFO @ Mon, 03 Jun 2019 21:46:12: 11000000 INFO @ Mon, 03 Jun 2019 21:46:12: 11000000 INFO @ Mon, 03 Jun 2019 21:46:16: 10000000 INFO @ Mon, 03 Jun 2019 21:46:20: 12000000 INFO @ Mon, 03 Jun 2019 21:46:20: 12000000 INFO @ Mon, 03 Jun 2019 21:46:26: 11000000 INFO @ Mon, 03 Jun 2019 21:46:29: 13000000 INFO @ Mon, 03 Jun 2019 21:46:29: 13000000 INFO @ Mon, 03 Jun 2019 21:46:36: 12000000 INFO @ Mon, 03 Jun 2019 21:46:37: 14000000 INFO @ Mon, 03 Jun 2019 21:46:37: 14000000 INFO @ Mon, 03 Jun 2019 21:46:46: 15000000 INFO @ Mon, 03 Jun 2019 21:46:46: 15000000 INFO @ Mon, 03 Jun 2019 21:46:46: 13000000 INFO @ Mon, 03 Jun 2019 21:46:54: 16000000 INFO @ Mon, 03 Jun 2019 21:46:54: 16000000 INFO @ Mon, 03 Jun 2019 21:46:56: 14000000 INFO @ Mon, 03 Jun 2019 21:47:03: 17000000 INFO @ Mon, 03 Jun 2019 21:47:03: 17000000 INFO @ Mon, 03 Jun 2019 21:47:06: 15000000 INFO @ Mon, 03 Jun 2019 21:47:12: 18000000 INFO @ Mon, 03 Jun 2019 21:47:12: 18000000 INFO @ Mon, 03 Jun 2019 21:47:15: 16000000 INFO @ Mon, 03 Jun 2019 21:47:20: 19000000 INFO @ Mon, 03 Jun 2019 21:47:20: 19000000 INFO @ Mon, 03 Jun 2019 21:47:25: 17000000 INFO @ Mon, 03 Jun 2019 21:47:29: 20000000 INFO @ Mon, 03 Jun 2019 21:47:29: 20000000 INFO @ Mon, 03 Jun 2019 21:47:34: 18000000 INFO @ Mon, 03 Jun 2019 21:47:38: 21000000 INFO @ Mon, 03 Jun 2019 21:47:38: 21000000 INFO @ Mon, 03 Jun 2019 21:47:44: 19000000 INFO @ Mon, 03 Jun 2019 21:47:47: 22000000 INFO @ Mon, 03 Jun 2019 21:47:47: 22000000 INFO @ Mon, 03 Jun 2019 21:47:54: 20000000 INFO @ Mon, 03 Jun 2019 21:47:55: 23000000 INFO @ Mon, 03 Jun 2019 21:47:55: 23000000 INFO @ Mon, 03 Jun 2019 21:47:58: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:47:58: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:47:58: #1 total tags in treatment: 23296738 INFO @ Mon, 03 Jun 2019 21:47:58: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:47:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:47:58: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:47:58: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:47:58: #1 total tags in treatment: 23296738 INFO @ Mon, 03 Jun 2019 21:47:58: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:47:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:47:59: #1 tags after filtering in treatment: 23296738 INFO @ Mon, 03 Jun 2019 21:47:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:47:59: #1 finished! INFO @ Mon, 03 Jun 2019 21:47:59: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:47:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:47:59: #1 tags after filtering in treatment: 23296738 INFO @ Mon, 03 Jun 2019 21:47:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:47:59: #1 finished! INFO @ Mon, 03 Jun 2019 21:47:59: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:47:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:48:01: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:48:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:48:01: Process for pairing-model is terminated! INFO @ Mon, 03 Jun 2019 21:48:01: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:48:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:48:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis cut: /home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.20_peaks.narrowPeak: No such file or directory needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:48:03: 21000000 INFO @ Mon, 03 Jun 2019 21:48:13: 22000000 INFO @ Mon, 03 Jun 2019 21:48:22: 23000000 INFO @ Mon, 03 Jun 2019 21:48:25: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:48:25: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:48:25: #1 total tags in treatment: 23296738 INFO @ Mon, 03 Jun 2019 21:48:25: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:48:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:48:26: #1 tags after filtering in treatment: 23296738 INFO @ Mon, 03 Jun 2019 21:48:26: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:48:26: #1 finished! INFO @ Mon, 03 Jun 2019 21:48:26: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:48:26: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:48:28: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 21:48:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:48:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495300/SRX495300.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。