Job ID = 2590642 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 30,968,303 reads read : 30,968,303 reads written : 30,968,303 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1198804.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:46 30968303 reads; of these: 30968303 (100.00%) were unpaired; of these: 3705038 (11.96%) aligned 0 times 21866368 (70.61%) aligned exactly 1 time 5396897 (17.43%) aligned >1 times 88.04% overall alignment rate Time searching: 00:08:46 Overall time: 00:08:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6865045 / 27263265 = 0.2518 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 23:08:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:08:40: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:08:40: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:08:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:08:41: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:08:41: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:08:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:08:42: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:08:42: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:08:50: 1000000 INFO @ Mon, 12 Aug 2019 23:08:52: 1000000 INFO @ Mon, 12 Aug 2019 23:08:54: 1000000 INFO @ Mon, 12 Aug 2019 23:08:59: 2000000 INFO @ Mon, 12 Aug 2019 23:09:03: 2000000 INFO @ Mon, 12 Aug 2019 23:09:05: 2000000 INFO @ Mon, 12 Aug 2019 23:09:09: 3000000 INFO @ Mon, 12 Aug 2019 23:09:14: 3000000 INFO @ Mon, 12 Aug 2019 23:09:16: 3000000 INFO @ Mon, 12 Aug 2019 23:09:18: 4000000 INFO @ Mon, 12 Aug 2019 23:09:25: 4000000 INFO @ Mon, 12 Aug 2019 23:09:27: 4000000 INFO @ Mon, 12 Aug 2019 23:09:27: 5000000 INFO @ Mon, 12 Aug 2019 23:09:35: 5000000 INFO @ Mon, 12 Aug 2019 23:09:36: 5000000 INFO @ Mon, 12 Aug 2019 23:09:38: 6000000 INFO @ Mon, 12 Aug 2019 23:09:45: 6000000 INFO @ Mon, 12 Aug 2019 23:09:46: 6000000 INFO @ Mon, 12 Aug 2019 23:09:48: 7000000 INFO @ Mon, 12 Aug 2019 23:09:54: 7000000 INFO @ Mon, 12 Aug 2019 23:09:57: 7000000 INFO @ Mon, 12 Aug 2019 23:09:59: 8000000 INFO @ Mon, 12 Aug 2019 23:10:03: 8000000 INFO @ Mon, 12 Aug 2019 23:10:07: 8000000 INFO @ Mon, 12 Aug 2019 23:10:10: 9000000 INFO @ Mon, 12 Aug 2019 23:10:13: 9000000 INFO @ Mon, 12 Aug 2019 23:10:18: 9000000 INFO @ Mon, 12 Aug 2019 23:10:20: 10000000 INFO @ Mon, 12 Aug 2019 23:10:22: 10000000 INFO @ Mon, 12 Aug 2019 23:10:29: 10000000 INFO @ Mon, 12 Aug 2019 23:10:31: 11000000 INFO @ Mon, 12 Aug 2019 23:10:31: 11000000 INFO @ Mon, 12 Aug 2019 23:10:39: 11000000 INFO @ Mon, 12 Aug 2019 23:10:40: 12000000 INFO @ Mon, 12 Aug 2019 23:10:41: 12000000 INFO @ Mon, 12 Aug 2019 23:10:51: 12000000 INFO @ Mon, 12 Aug 2019 23:10:52: 13000000 INFO @ Mon, 12 Aug 2019 23:10:55: 13000000 INFO @ Mon, 12 Aug 2019 23:11:03: 14000000 INFO @ Mon, 12 Aug 2019 23:11:04: 13000000 INFO @ Mon, 12 Aug 2019 23:11:06: 14000000 INFO @ Mon, 12 Aug 2019 23:11:12: 15000000 INFO @ Mon, 12 Aug 2019 23:11:14: 14000000 INFO @ Mon, 12 Aug 2019 23:11:16: 15000000 INFO @ Mon, 12 Aug 2019 23:11:21: 16000000 INFO @ Mon, 12 Aug 2019 23:11:24: 15000000 INFO @ Mon, 12 Aug 2019 23:11:26: 16000000 INFO @ Mon, 12 Aug 2019 23:11:30: 17000000 INFO @ Mon, 12 Aug 2019 23:11:35: 16000000 INFO @ Mon, 12 Aug 2019 23:11:37: 17000000 INFO @ Mon, 12 Aug 2019 23:11:40: 18000000 INFO @ Mon, 12 Aug 2019 23:11:45: 17000000 INFO @ Mon, 12 Aug 2019 23:11:48: 18000000 INFO @ Mon, 12 Aug 2019 23:11:49: 19000000 INFO @ Mon, 12 Aug 2019 23:11:56: 18000000 INFO @ Mon, 12 Aug 2019 23:11:58: 19000000 INFO @ Mon, 12 Aug 2019 23:11:58: 20000000 INFO @ Mon, 12 Aug 2019 23:12:02: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:12:02: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:12:02: #1 total tags in treatment: 20398220 INFO @ Mon, 12 Aug 2019 23:12:02: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:12:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:12:02: #1 tags after filtering in treatment: 20398220 INFO @ Mon, 12 Aug 2019 23:12:02: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:12:02: #1 finished! INFO @ Mon, 12 Aug 2019 23:12:02: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:12:02: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:12:04: #2 number of paired peaks: 39 WARNING @ Mon, 12 Aug 2019 23:12:04: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:12:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:12:06: 19000000 INFO @ Mon, 12 Aug 2019 23:12:08: 20000000 INFO @ Mon, 12 Aug 2019 23:12:12: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:12:12: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:12:12: #1 total tags in treatment: 20398220 INFO @ Mon, 12 Aug 2019 23:12:12: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:12:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:12:12: #1 tags after filtering in treatment: 20398220 INFO @ Mon, 12 Aug 2019 23:12:12: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:12:12: #1 finished! INFO @ Mon, 12 Aug 2019 23:12:12: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:12:12: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:12:14: #2 number of paired peaks: 39 WARNING @ Mon, 12 Aug 2019 23:12:14: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:12:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:12:15: 20000000 INFO @ Mon, 12 Aug 2019 23:12:18: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:12:18: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:12:18: #1 total tags in treatment: 20398220 INFO @ Mon, 12 Aug 2019 23:12:18: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:12:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:12:19: #1 tags after filtering in treatment: 20398220 INFO @ Mon, 12 Aug 2019 23:12:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:12:19: #1 finished! INFO @ Mon, 12 Aug 2019 23:12:19: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:12:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:12:21: #2 number of paired peaks: 39 WARNING @ Mon, 12 Aug 2019 23:12:21: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:12:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495299/SRX495299.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。