Job ID = 2590641 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 27,086,977 reads read : 27,086,977 reads written : 27,086,977 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1198803.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:54 27086977 reads; of these: 27086977 (100.00%) were unpaired; of these: 3739077 (13.80%) aligned 0 times 20134151 (74.33%) aligned exactly 1 time 3213749 (11.86%) aligned >1 times 86.20% overall alignment rate Time searching: 00:06:54 Overall time: 00:06:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6944524 / 23347900 = 0.2974 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 23:05:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:05:26: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:05:26: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:05:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:05:26: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:05:26: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:05:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 23:05:27: #1 read tag files... INFO @ Mon, 12 Aug 2019 23:05:27: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 23:05:34: 1000000 INFO @ Mon, 12 Aug 2019 23:05:34: 1000000 INFO @ Mon, 12 Aug 2019 23:05:36: 1000000 INFO @ Mon, 12 Aug 2019 23:05:40: 2000000 INFO @ Mon, 12 Aug 2019 23:05:41: 2000000 INFO @ Mon, 12 Aug 2019 23:05:44: 2000000 INFO @ Mon, 12 Aug 2019 23:05:47: 3000000 INFO @ Mon, 12 Aug 2019 23:05:49: 3000000 INFO @ Mon, 12 Aug 2019 23:05:51: 3000000 INFO @ Mon, 12 Aug 2019 23:05:53: 4000000 INFO @ Mon, 12 Aug 2019 23:05:56: 4000000 INFO @ Mon, 12 Aug 2019 23:05:59: 4000000 INFO @ Mon, 12 Aug 2019 23:05:59: 5000000 INFO @ Mon, 12 Aug 2019 23:06:04: 5000000 INFO @ Mon, 12 Aug 2019 23:06:06: 6000000 INFO @ Mon, 12 Aug 2019 23:06:07: 5000000 INFO @ Mon, 12 Aug 2019 23:06:11: 6000000 INFO @ Mon, 12 Aug 2019 23:06:12: 7000000 INFO @ Mon, 12 Aug 2019 23:06:14: 6000000 INFO @ Mon, 12 Aug 2019 23:06:18: 8000000 INFO @ Mon, 12 Aug 2019 23:06:19: 7000000 INFO @ Mon, 12 Aug 2019 23:06:22: 7000000 INFO @ Mon, 12 Aug 2019 23:06:25: 9000000 INFO @ Mon, 12 Aug 2019 23:06:26: 8000000 INFO @ Mon, 12 Aug 2019 23:06:30: 8000000 INFO @ Mon, 12 Aug 2019 23:06:31: 10000000 INFO @ Mon, 12 Aug 2019 23:06:34: 9000000 INFO @ Mon, 12 Aug 2019 23:06:37: 11000000 INFO @ Mon, 12 Aug 2019 23:06:38: 9000000 INFO @ Mon, 12 Aug 2019 23:06:41: 10000000 INFO @ Mon, 12 Aug 2019 23:06:43: 12000000 INFO @ Mon, 12 Aug 2019 23:06:45: 10000000 INFO @ Mon, 12 Aug 2019 23:06:49: 11000000 INFO @ Mon, 12 Aug 2019 23:06:50: 13000000 INFO @ Mon, 12 Aug 2019 23:06:53: 11000000 INFO @ Mon, 12 Aug 2019 23:06:56: 12000000 INFO @ Mon, 12 Aug 2019 23:06:56: 14000000 INFO @ Mon, 12 Aug 2019 23:07:00: 12000000 INFO @ Mon, 12 Aug 2019 23:07:03: 15000000 INFO @ Mon, 12 Aug 2019 23:07:04: 13000000 INFO @ Mon, 12 Aug 2019 23:07:08: 13000000 INFO @ Mon, 12 Aug 2019 23:07:09: 16000000 INFO @ Mon, 12 Aug 2019 23:07:11: 14000000 INFO @ Mon, 12 Aug 2019 23:07:12: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:07:12: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:07:12: #1 total tags in treatment: 16403376 INFO @ Mon, 12 Aug 2019 23:07:12: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:07:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:07:12: #1 tags after filtering in treatment: 16403376 INFO @ Mon, 12 Aug 2019 23:07:12: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:07:12: #1 finished! INFO @ Mon, 12 Aug 2019 23:07:12: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:07:12: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:07:14: #2 number of paired peaks: 10 WARNING @ Mon, 12 Aug 2019 23:07:14: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:07:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:07:16: 14000000 INFO @ Mon, 12 Aug 2019 23:07:19: 15000000 INFO @ Mon, 12 Aug 2019 23:07:24: 15000000 INFO @ Mon, 12 Aug 2019 23:07:26: 16000000 INFO @ Mon, 12 Aug 2019 23:07:29: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:07:29: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:07:29: #1 total tags in treatment: 16403376 INFO @ Mon, 12 Aug 2019 23:07:29: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:07:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:07:29: #1 tags after filtering in treatment: 16403376 INFO @ Mon, 12 Aug 2019 23:07:29: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:07:29: #1 finished! INFO @ Mon, 12 Aug 2019 23:07:29: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:07:29: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:07:31: #2 number of paired peaks: 10 WARNING @ Mon, 12 Aug 2019 23:07:31: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:07:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 23:07:31: 16000000 INFO @ Mon, 12 Aug 2019 23:07:34: #1 tag size is determined as 50 bps INFO @ Mon, 12 Aug 2019 23:07:34: #1 tag size = 50 INFO @ Mon, 12 Aug 2019 23:07:34: #1 total tags in treatment: 16403376 INFO @ Mon, 12 Aug 2019 23:07:34: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 23:07:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 23:07:35: #1 tags after filtering in treatment: 16403376 INFO @ Mon, 12 Aug 2019 23:07:35: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 23:07:35: #1 finished! INFO @ Mon, 12 Aug 2019 23:07:35: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 23:07:35: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 23:07:36: #2 number of paired peaks: 10 WARNING @ Mon, 12 Aug 2019 23:07:36: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 23:07:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495298/SRX495298.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。