Job ID = 6528271 SRX = SRX495294 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:31:21 prefetch.2.10.7: 1) Downloading 'SRR1198799'... 2020-06-29T14:31:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:36:05 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:36:05 prefetch.2.10.7: 1) 'SRR1198799' was downloaded successfully Read 28429556 spots for SRR1198799/SRR1198799.sra Written 28429556 spots for SRR1198799/SRR1198799.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:19 28429556 reads; of these: 28429556 (100.00%) were unpaired; of these: 10256805 (36.08%) aligned 0 times 13954833 (49.09%) aligned exactly 1 time 4217918 (14.84%) aligned >1 times 63.92% overall alignment rate Time searching: 00:06:19 Overall time: 00:06:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3905784 / 18172751 = 0.2149 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:54:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:54:53: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:54:53: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:54:59: 1000000 INFO @ Mon, 29 Jun 2020 23:55:05: 2000000 INFO @ Mon, 29 Jun 2020 23:55:12: 3000000 INFO @ Mon, 29 Jun 2020 23:55:18: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:55:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:55:22: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:55:22: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:55:25: 5000000 INFO @ Mon, 29 Jun 2020 23:55:29: 1000000 INFO @ Mon, 29 Jun 2020 23:55:31: 6000000 INFO @ Mon, 29 Jun 2020 23:55:35: 2000000 INFO @ Mon, 29 Jun 2020 23:55:38: 7000000 INFO @ Mon, 29 Jun 2020 23:55:41: 3000000 INFO @ Mon, 29 Jun 2020 23:55:45: 8000000 INFO @ Mon, 29 Jun 2020 23:55:48: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:55:52: 9000000 INFO @ Mon, 29 Jun 2020 23:55:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:55:52: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:55:52: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:55:54: 5000000 INFO @ Mon, 29 Jun 2020 23:55:59: 10000000 INFO @ Mon, 29 Jun 2020 23:55:59: 1000000 INFO @ Mon, 29 Jun 2020 23:56:00: 6000000 INFO @ Mon, 29 Jun 2020 23:56:06: 2000000 INFO @ Mon, 29 Jun 2020 23:56:06: 11000000 INFO @ Mon, 29 Jun 2020 23:56:06: 7000000 INFO @ Mon, 29 Jun 2020 23:56:13: 8000000 INFO @ Mon, 29 Jun 2020 23:56:13: 3000000 INFO @ Mon, 29 Jun 2020 23:56:13: 12000000 INFO @ Mon, 29 Jun 2020 23:56:19: 9000000 INFO @ Mon, 29 Jun 2020 23:56:19: 4000000 INFO @ Mon, 29 Jun 2020 23:56:21: 13000000 INFO @ Mon, 29 Jun 2020 23:56:25: 10000000 INFO @ Mon, 29 Jun 2020 23:56:26: 5000000 INFO @ Mon, 29 Jun 2020 23:56:28: 14000000 INFO @ Mon, 29 Jun 2020 23:56:30: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:56:30: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:56:30: #1 total tags in treatment: 14266967 INFO @ Mon, 29 Jun 2020 23:56:30: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:56:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:56:30: #1 tags after filtering in treatment: 14266967 INFO @ Mon, 29 Jun 2020 23:56:30: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:56:30: #1 finished! INFO @ Mon, 29 Jun 2020 23:56:30: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:56:30: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:56:31: #2 number of paired peaks: 46 WARNING @ Mon, 29 Jun 2020 23:56:31: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:56:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:56:32: 11000000 INFO @ Mon, 29 Jun 2020 23:56:32: 6000000 INFO @ Mon, 29 Jun 2020 23:56:38: 12000000 INFO @ Mon, 29 Jun 2020 23:56:38: 7000000 INFO @ Mon, 29 Jun 2020 23:56:44: 13000000 INFO @ Mon, 29 Jun 2020 23:56:44: 8000000 INFO @ Mon, 29 Jun 2020 23:56:50: 14000000 INFO @ Mon, 29 Jun 2020 23:56:51: 9000000 INFO @ Mon, 29 Jun 2020 23:56:52: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:56:52: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:56:52: #1 total tags in treatment: 14266967 INFO @ Mon, 29 Jun 2020 23:56:52: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:56:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:56:52: #1 tags after filtering in treatment: 14266967 INFO @ Mon, 29 Jun 2020 23:56:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:56:52: #1 finished! INFO @ Mon, 29 Jun 2020 23:56:52: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:56:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:56:53: #2 number of paired peaks: 46 WARNING @ Mon, 29 Jun 2020 23:56:53: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:56:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:56:57: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:57:03: 11000000 INFO @ Mon, 29 Jun 2020 23:57:09: 12000000 INFO @ Mon, 29 Jun 2020 23:57:14: 13000000 INFO @ Mon, 29 Jun 2020 23:57:20: 14000000 INFO @ Mon, 29 Jun 2020 23:57:22: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:57:22: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:57:22: #1 total tags in treatment: 14266967 INFO @ Mon, 29 Jun 2020 23:57:22: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:57:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:57:22: #1 tags after filtering in treatment: 14266967 INFO @ Mon, 29 Jun 2020 23:57:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:57:22: #1 finished! INFO @ Mon, 29 Jun 2020 23:57:22: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:57:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:57:23: #2 number of paired peaks: 46 WARNING @ Mon, 29 Jun 2020 23:57:23: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:57:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495294/SRX495294.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。