Job ID = 6498426 SRX = SRX495283 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:37:00 prefetch.2.10.7: 1) Downloading 'SRR1198788'... 2020-06-25T23:37:00 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:45:01 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:45:01 prefetch.2.10.7: 1) 'SRR1198788' was downloaded successfully Read 55261147 spots for SRR1198788/SRR1198788.sra Written 55261147 spots for SRR1198788/SRR1198788.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:19 55261147 reads; of these: 55261147 (100.00%) were unpaired; of these: 1067031 (1.93%) aligned 0 times 45427132 (82.20%) aligned exactly 1 time 8766984 (15.86%) aligned >1 times 98.07% overall alignment rate Time searching: 00:16:19 Overall time: 00:16:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 16998450 / 54194116 = 0.3137 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:16:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:16:17: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:16:17: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:16:23: 1000000 INFO @ Fri, 26 Jun 2020 09:16:29: 2000000 INFO @ Fri, 26 Jun 2020 09:16:35: 3000000 INFO @ Fri, 26 Jun 2020 09:16:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:16:46: 5000000 INFO @ Fri, 26 Jun 2020 09:16:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:16:47: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:16:47: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:16:53: 6000000 INFO @ Fri, 26 Jun 2020 09:16:54: 1000000 INFO @ Fri, 26 Jun 2020 09:16:59: 7000000 INFO @ Fri, 26 Jun 2020 09:17:01: 2000000 INFO @ Fri, 26 Jun 2020 09:17:06: 8000000 INFO @ Fri, 26 Jun 2020 09:17:09: 3000000 INFO @ Fri, 26 Jun 2020 09:17:12: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:17:16: 4000000 INFO @ Fri, 26 Jun 2020 09:17:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:17:17: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:17:17: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:17:18: 10000000 INFO @ Fri, 26 Jun 2020 09:17:23: 5000000 INFO @ Fri, 26 Jun 2020 09:17:24: 1000000 INFO @ Fri, 26 Jun 2020 09:17:25: 11000000 INFO @ Fri, 26 Jun 2020 09:17:31: 6000000 INFO @ Fri, 26 Jun 2020 09:17:31: 12000000 INFO @ Fri, 26 Jun 2020 09:17:32: 2000000 INFO @ Fri, 26 Jun 2020 09:17:37: 13000000 INFO @ Fri, 26 Jun 2020 09:17:38: 7000000 INFO @ Fri, 26 Jun 2020 09:17:39: 3000000 INFO @ Fri, 26 Jun 2020 09:17:44: 14000000 INFO @ Fri, 26 Jun 2020 09:17:45: 8000000 INFO @ Fri, 26 Jun 2020 09:17:47: 4000000 INFO @ Fri, 26 Jun 2020 09:17:50: 15000000 INFO @ Fri, 26 Jun 2020 09:17:53: 9000000 INFO @ Fri, 26 Jun 2020 09:17:54: 5000000 INFO @ Fri, 26 Jun 2020 09:17:57: 16000000 INFO @ Fri, 26 Jun 2020 09:18:00: 10000000 INFO @ Fri, 26 Jun 2020 09:18:01: 6000000 INFO @ Fri, 26 Jun 2020 09:18:03: 17000000 INFO @ Fri, 26 Jun 2020 09:18:07: 11000000 INFO @ Fri, 26 Jun 2020 09:18:09: 7000000 INFO @ Fri, 26 Jun 2020 09:18:09: 18000000 INFO @ Fri, 26 Jun 2020 09:18:14: 12000000 INFO @ Fri, 26 Jun 2020 09:18:16: 19000000 INFO @ Fri, 26 Jun 2020 09:18:16: 8000000 INFO @ Fri, 26 Jun 2020 09:18:21: 13000000 INFO @ Fri, 26 Jun 2020 09:18:22: 20000000 INFO @ Fri, 26 Jun 2020 09:18:23: 9000000 INFO @ Fri, 26 Jun 2020 09:18:28: 14000000 INFO @ Fri, 26 Jun 2020 09:18:29: 21000000 INFO @ Fri, 26 Jun 2020 09:18:30: 10000000 INFO @ Fri, 26 Jun 2020 09:18:35: 22000000 INFO @ Fri, 26 Jun 2020 09:18:36: 15000000 INFO @ Fri, 26 Jun 2020 09:18:37: 11000000 INFO @ Fri, 26 Jun 2020 09:18:42: 23000000 INFO @ Fri, 26 Jun 2020 09:18:43: 16000000 INFO @ Fri, 26 Jun 2020 09:18:45: 12000000 INFO @ Fri, 26 Jun 2020 09:18:48: 24000000 INFO @ Fri, 26 Jun 2020 09:18:50: 17000000 INFO @ Fri, 26 Jun 2020 09:18:52: 13000000 INFO @ Fri, 26 Jun 2020 09:18:54: 25000000 INFO @ Fri, 26 Jun 2020 09:18:57: 18000000 INFO @ Fri, 26 Jun 2020 09:18:59: 14000000 INFO @ Fri, 26 Jun 2020 09:19:01: 26000000 INFO @ Fri, 26 Jun 2020 09:19:04: 19000000 INFO @ Fri, 26 Jun 2020 09:19:06: 15000000 INFO @ Fri, 26 Jun 2020 09:19:07: 27000000 INFO @ Fri, 26 Jun 2020 09:19:12: 20000000 INFO @ Fri, 26 Jun 2020 09:19:13: 16000000 INFO @ Fri, 26 Jun 2020 09:19:14: 28000000 INFO @ Fri, 26 Jun 2020 09:19:19: 21000000 INFO @ Fri, 26 Jun 2020 09:19:21: 17000000 INFO @ Fri, 26 Jun 2020 09:19:21: 29000000 INFO @ Fri, 26 Jun 2020 09:19:26: 22000000 INFO @ Fri, 26 Jun 2020 09:19:27: 30000000 INFO @ Fri, 26 Jun 2020 09:19:28: 18000000 INFO @ Fri, 26 Jun 2020 09:19:32: 23000000 INFO @ Fri, 26 Jun 2020 09:19:34: 31000000 INFO @ Fri, 26 Jun 2020 09:19:35: 19000000 INFO @ Fri, 26 Jun 2020 09:19:39: 24000000 INFO @ Fri, 26 Jun 2020 09:19:41: 32000000 INFO @ Fri, 26 Jun 2020 09:19:42: 20000000 INFO @ Fri, 26 Jun 2020 09:19:46: 25000000 INFO @ Fri, 26 Jun 2020 09:19:47: 33000000 INFO @ Fri, 26 Jun 2020 09:19:50: 21000000 INFO @ Fri, 26 Jun 2020 09:19:53: 26000000 INFO @ Fri, 26 Jun 2020 09:19:54: 34000000 INFO @ Fri, 26 Jun 2020 09:19:57: 22000000 INFO @ Fri, 26 Jun 2020 09:20:00: 27000000 INFO @ Fri, 26 Jun 2020 09:20:01: 35000000 INFO @ Fri, 26 Jun 2020 09:20:04: 23000000 INFO @ Fri, 26 Jun 2020 09:20:06: 28000000 INFO @ Fri, 26 Jun 2020 09:20:08: 36000000 INFO @ Fri, 26 Jun 2020 09:20:12: 24000000 INFO @ Fri, 26 Jun 2020 09:20:13: 29000000 INFO @ Fri, 26 Jun 2020 09:20:15: 37000000 INFO @ Fri, 26 Jun 2020 09:20:17: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 09:20:17: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 09:20:17: #1 total tags in treatment: 37195666 INFO @ Fri, 26 Jun 2020 09:20:17: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:20:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:20:18: #1 tags after filtering in treatment: 37195666 INFO @ Fri, 26 Jun 2020 09:20:18: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:20:18: #1 finished! INFO @ Fri, 26 Jun 2020 09:20:18: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:20:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:20:19: 25000000 INFO @ Fri, 26 Jun 2020 09:20:20: 30000000 INFO @ Fri, 26 Jun 2020 09:20:20: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 09:20:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:20:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:20:26: 26000000 INFO @ Fri, 26 Jun 2020 09:20:27: 31000000 INFO @ Fri, 26 Jun 2020 09:20:33: 27000000 INFO @ Fri, 26 Jun 2020 09:20:33: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:20:40: 33000000 INFO @ Fri, 26 Jun 2020 09:20:41: 28000000 INFO @ Fri, 26 Jun 2020 09:20:47: 34000000 INFO @ Fri, 26 Jun 2020 09:20:48: 29000000 INFO @ Fri, 26 Jun 2020 09:20:54: 35000000 INFO @ Fri, 26 Jun 2020 09:20:56: 30000000 INFO @ Fri, 26 Jun 2020 09:21:01: 36000000 INFO @ Fri, 26 Jun 2020 09:21:03: 31000000 INFO @ Fri, 26 Jun 2020 09:21:08: 37000000 INFO @ Fri, 26 Jun 2020 09:21:09: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 09:21:09: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 09:21:09: #1 total tags in treatment: 37195666 INFO @ Fri, 26 Jun 2020 09:21:09: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:21:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:21:10: #1 tags after filtering in treatment: 37195666 INFO @ Fri, 26 Jun 2020 09:21:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:21:10: #1 finished! INFO @ Fri, 26 Jun 2020 09:21:10: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:21:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:21:10: 32000000 INFO @ Fri, 26 Jun 2020 09:21:13: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 09:21:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:21:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:21:17: 33000000 INFO @ Fri, 26 Jun 2020 09:21:24: 34000000 INFO @ Fri, 26 Jun 2020 09:21:31: 35000000 INFO @ Fri, 26 Jun 2020 09:21:39: 36000000 INFO @ Fri, 26 Jun 2020 09:21:46: 37000000 INFO @ Fri, 26 Jun 2020 09:21:48: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 09:21:48: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 09:21:48: #1 total tags in treatment: 37195666 INFO @ Fri, 26 Jun 2020 09:21:48: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:21:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:21:48: #1 tags after filtering in treatment: 37195666 INFO @ Fri, 26 Jun 2020 09:21:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:21:48: #1 finished! INFO @ Fri, 26 Jun 2020 09:21:48: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:21:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:21:51: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 09:21:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:21:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495283/SRX495283.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。