Job ID = 6498422 SRX = SRX495279 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:26:46 prefetch.2.10.7: 1) Downloading 'SRR1198784'... 2020-06-25T23:26:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:29:01 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:29:01 prefetch.2.10.7: 1) 'SRR1198784' was downloaded successfully Read 17765478 spots for SRR1198784/SRR1198784.sra Written 17765478 spots for SRR1198784/SRR1198784.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:52 17765478 reads; of these: 17765478 (100.00%) were unpaired; of these: 535852 (3.02%) aligned 0 times 14657771 (82.51%) aligned exactly 1 time 2571855 (14.48%) aligned >1 times 96.98% overall alignment rate Time searching: 00:04:52 Overall time: 00:04:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2660137 / 17229626 = 0.1544 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:39:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:39:01: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:39:01: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:39:06: 1000000 INFO @ Fri, 26 Jun 2020 08:39:12: 2000000 INFO @ Fri, 26 Jun 2020 08:39:18: 3000000 INFO @ Fri, 26 Jun 2020 08:39:23: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:39:29: 5000000 INFO @ Fri, 26 Jun 2020 08:39:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:39:31: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:39:31: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:39:35: 6000000 INFO @ Fri, 26 Jun 2020 08:39:37: 1000000 INFO @ Fri, 26 Jun 2020 08:39:41: 7000000 INFO @ Fri, 26 Jun 2020 08:39:43: 2000000 INFO @ Fri, 26 Jun 2020 08:39:48: 8000000 INFO @ Fri, 26 Jun 2020 08:39:50: 3000000 INFO @ Fri, 26 Jun 2020 08:39:54: 9000000 INFO @ Fri, 26 Jun 2020 08:39:56: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:40:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:40:01: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:40:01: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:40:01: 10000000 INFO @ Fri, 26 Jun 2020 08:40:03: 5000000 INFO @ Fri, 26 Jun 2020 08:40:07: 1000000 INFO @ Fri, 26 Jun 2020 08:40:07: 11000000 INFO @ Fri, 26 Jun 2020 08:40:09: 6000000 INFO @ Fri, 26 Jun 2020 08:40:14: 2000000 INFO @ Fri, 26 Jun 2020 08:40:14: 12000000 INFO @ Fri, 26 Jun 2020 08:40:16: 7000000 INFO @ Fri, 26 Jun 2020 08:40:21: 13000000 INFO @ Fri, 26 Jun 2020 08:40:21: 3000000 INFO @ Fri, 26 Jun 2020 08:40:22: 8000000 INFO @ Fri, 26 Jun 2020 08:40:27: 14000000 INFO @ Fri, 26 Jun 2020 08:40:28: 4000000 INFO @ Fri, 26 Jun 2020 08:40:29: 9000000 INFO @ Fri, 26 Jun 2020 08:40:31: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:40:31: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:40:31: #1 total tags in treatment: 14569489 INFO @ Fri, 26 Jun 2020 08:40:31: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:40:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:40:32: #1 tags after filtering in treatment: 14569489 INFO @ Fri, 26 Jun 2020 08:40:32: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:40:32: #1 finished! INFO @ Fri, 26 Jun 2020 08:40:32: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:40:32: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:40:33: #2 number of paired peaks: 36 WARNING @ Fri, 26 Jun 2020 08:40:33: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:40:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:40:34: 5000000 INFO @ Fri, 26 Jun 2020 08:40:36: 10000000 INFO @ Fri, 26 Jun 2020 08:40:41: 6000000 INFO @ Fri, 26 Jun 2020 08:40:42: 11000000 INFO @ Fri, 26 Jun 2020 08:40:48: 7000000 INFO @ Fri, 26 Jun 2020 08:40:49: 12000000 INFO @ Fri, 26 Jun 2020 08:40:55: 8000000 INFO @ Fri, 26 Jun 2020 08:40:55: 13000000 INFO @ Fri, 26 Jun 2020 08:41:01: 9000000 INFO @ Fri, 26 Jun 2020 08:41:02: 14000000 INFO @ Fri, 26 Jun 2020 08:41:06: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:41:06: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:41:06: #1 total tags in treatment: 14569489 INFO @ Fri, 26 Jun 2020 08:41:06: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:41:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:41:06: #1 tags after filtering in treatment: 14569489 INFO @ Fri, 26 Jun 2020 08:41:06: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:41:06: #1 finished! INFO @ Fri, 26 Jun 2020 08:41:06: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:41:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:41:07: #2 number of paired peaks: 36 WARNING @ Fri, 26 Jun 2020 08:41:07: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:41:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:41:08: 10000000 INFO @ Fri, 26 Jun 2020 08:41:15: 11000000 INFO @ Fri, 26 Jun 2020 08:41:21: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:41:28: 13000000 INFO @ Fri, 26 Jun 2020 08:41:34: 14000000 INFO @ Fri, 26 Jun 2020 08:41:37: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:41:37: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:41:37: #1 total tags in treatment: 14569489 INFO @ Fri, 26 Jun 2020 08:41:37: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:41:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:41:38: #1 tags after filtering in treatment: 14569489 INFO @ Fri, 26 Jun 2020 08:41:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:41:38: #1 finished! INFO @ Fri, 26 Jun 2020 08:41:38: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:41:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:41:39: #2 number of paired peaks: 36 WARNING @ Fri, 26 Jun 2020 08:41:39: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:41:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495279/SRX495279.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。