Job ID = 1305237 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 11,648,528 reads read : 11,648,528 reads written : 11,648,528 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:50 11648528 reads; of these: 11648528 (100.00%) were unpaired; of these: 536100 (4.60%) aligned 0 times 8487838 (72.87%) aligned exactly 1 time 2624590 (22.53%) aligned >1 times 95.40% overall alignment rate Time searching: 00:04:50 Overall time: 00:04:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1764825 / 11112428 = 0.1588 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:18:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:18:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:18:48: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:18:48: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:18:48: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:18:48: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:18:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:18:48: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:18:48: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:18:59: 1000000 INFO @ Mon, 03 Jun 2019 21:19:00: 1000000 INFO @ Mon, 03 Jun 2019 21:19:01: 1000000 INFO @ Mon, 03 Jun 2019 21:19:11: 2000000 INFO @ Mon, 03 Jun 2019 21:19:13: 2000000 INFO @ Mon, 03 Jun 2019 21:19:14: 2000000 INFO @ Mon, 03 Jun 2019 21:19:22: 3000000 INFO @ Mon, 03 Jun 2019 21:19:25: 3000000 INFO @ Mon, 03 Jun 2019 21:19:26: 3000000 INFO @ Mon, 03 Jun 2019 21:19:33: 4000000 INFO @ Mon, 03 Jun 2019 21:19:36: 4000000 INFO @ Mon, 03 Jun 2019 21:19:39: 4000000 INFO @ Mon, 03 Jun 2019 21:19:45: 5000000 INFO @ Mon, 03 Jun 2019 21:19:48: 5000000 INFO @ Mon, 03 Jun 2019 21:19:51: 5000000 INFO @ Mon, 03 Jun 2019 21:19:58: 6000000 INFO @ Mon, 03 Jun 2019 21:19:59: 6000000 INFO @ Mon, 03 Jun 2019 21:20:03: 6000000 INFO @ Mon, 03 Jun 2019 21:20:10: 7000000 INFO @ Mon, 03 Jun 2019 21:20:11: 7000000 INFO @ Mon, 03 Jun 2019 21:20:16: 7000000 INFO @ Mon, 03 Jun 2019 21:20:21: 8000000 INFO @ Mon, 03 Jun 2019 21:20:23: 8000000 INFO @ Mon, 03 Jun 2019 21:20:28: 8000000 INFO @ Mon, 03 Jun 2019 21:20:32: 9000000 INFO @ Mon, 03 Jun 2019 21:20:35: 9000000 INFO @ Mon, 03 Jun 2019 21:20:35: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:20:35: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:20:35: #1 total tags in treatment: 9347603 INFO @ Mon, 03 Jun 2019 21:20:35: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:20:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:20:36: #1 tags after filtering in treatment: 9347603 INFO @ Mon, 03 Jun 2019 21:20:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:20:36: #1 finished! INFO @ Mon, 03 Jun 2019 21:20:36: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:20:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:20:37: #2 number of paired peaks: 46 WARNING @ Mon, 03 Jun 2019 21:20:37: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:20:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:20:39: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:20:39: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:20:39: #1 total tags in treatment: 9347603 INFO @ Mon, 03 Jun 2019 21:20:39: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:20:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:20:39: #1 tags after filtering in treatment: 9347603 INFO @ Mon, 03 Jun 2019 21:20:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:20:39: #1 finished! INFO @ Mon, 03 Jun 2019 21:20:39: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:20:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:20:40: #2 number of paired peaks: 46 WARNING @ Mon, 03 Jun 2019 21:20:40: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:20:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:20:41: 9000000 INFO @ Mon, 03 Jun 2019 21:20:45: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:20:45: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:20:45: #1 total tags in treatment: 9347603 INFO @ Mon, 03 Jun 2019 21:20:45: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:20:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:20:45: #1 tags after filtering in treatment: 9347603 INFO @ Mon, 03 Jun 2019 21:20:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:20:45: #1 finished! INFO @ Mon, 03 Jun 2019 21:20:45: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:20:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:20:46: #2 number of paired peaks: 46 WARNING @ Mon, 03 Jun 2019 21:20:46: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:20:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495273/SRX495273.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。