Job ID = 1305224 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 84,993,195 reads read : 84,993,195 reads written : 84,993,195 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:30:54 84993195 reads; of these: 84993195 (100.00%) were unpaired; of these: 2870153 (3.38%) aligned 0 times 60614243 (71.32%) aligned exactly 1 time 21508799 (25.31%) aligned >1 times 96.62% overall alignment rate Time searching: 00:30:54 Overall time: 00:30:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 34981883 / 82123042 = 0.4260 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 22:05:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:05:41: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:05:41: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:05:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:05:41: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:05:41: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:05:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 22:05:41: #1 read tag files... INFO @ Mon, 03 Jun 2019 22:05:41: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 22:05:49: 1000000 INFO @ Mon, 03 Jun 2019 22:05:50: 1000000 INFO @ Mon, 03 Jun 2019 22:05:50: 1000000 INFO @ Mon, 03 Jun 2019 22:05:57: 2000000 INFO @ Mon, 03 Jun 2019 22:05:59: 2000000 INFO @ Mon, 03 Jun 2019 22:06:00: 2000000 INFO @ Mon, 03 Jun 2019 22:06:05: 3000000 INFO @ Mon, 03 Jun 2019 22:06:08: 3000000 INFO @ Mon, 03 Jun 2019 22:06:09: 3000000 INFO @ Mon, 03 Jun 2019 22:06:12: 4000000 INFO @ Mon, 03 Jun 2019 22:06:16: 4000000 INFO @ Mon, 03 Jun 2019 22:06:18: 4000000 INFO @ Mon, 03 Jun 2019 22:06:20: 5000000 INFO @ Mon, 03 Jun 2019 22:06:25: 5000000 INFO @ Mon, 03 Jun 2019 22:06:27: 5000000 INFO @ Mon, 03 Jun 2019 22:06:27: 6000000 INFO @ Mon, 03 Jun 2019 22:06:33: 6000000 INFO @ Mon, 03 Jun 2019 22:06:35: 7000000 INFO @ Mon, 03 Jun 2019 22:06:36: 6000000 INFO @ Mon, 03 Jun 2019 22:06:42: 7000000 INFO @ Mon, 03 Jun 2019 22:06:43: 8000000 INFO @ Mon, 03 Jun 2019 22:06:45: 7000000 INFO @ Mon, 03 Jun 2019 22:06:51: 8000000 INFO @ Mon, 03 Jun 2019 22:06:51: 9000000 INFO @ Mon, 03 Jun 2019 22:06:54: 8000000 INFO @ Mon, 03 Jun 2019 22:06:59: 10000000 INFO @ Mon, 03 Jun 2019 22:06:59: 9000000 INFO @ Mon, 03 Jun 2019 22:07:03: 9000000 INFO @ Mon, 03 Jun 2019 22:07:07: 11000000 INFO @ Mon, 03 Jun 2019 22:07:08: 10000000 INFO @ Mon, 03 Jun 2019 22:07:13: 10000000 INFO @ Mon, 03 Jun 2019 22:07:15: 12000000 INFO @ Mon, 03 Jun 2019 22:07:17: 11000000 INFO @ Mon, 03 Jun 2019 22:07:22: 11000000 INFO @ Mon, 03 Jun 2019 22:07:24: 13000000 INFO @ Mon, 03 Jun 2019 22:07:26: 12000000 INFO @ Mon, 03 Jun 2019 22:07:31: 12000000 INFO @ Mon, 03 Jun 2019 22:07:32: 14000000 INFO @ Mon, 03 Jun 2019 22:07:34: 13000000 INFO @ Mon, 03 Jun 2019 22:07:40: 13000000 INFO @ Mon, 03 Jun 2019 22:07:40: 15000000 INFO @ Mon, 03 Jun 2019 22:07:42: 14000000 INFO @ Mon, 03 Jun 2019 22:07:48: 16000000 INFO @ Mon, 03 Jun 2019 22:07:48: 14000000 INFO @ Mon, 03 Jun 2019 22:07:51: 15000000 INFO @ Mon, 03 Jun 2019 22:07:56: 17000000 INFO @ Mon, 03 Jun 2019 22:07:57: 15000000 INFO @ Mon, 03 Jun 2019 22:07:59: 16000000 INFO @ Mon, 03 Jun 2019 22:08:05: 18000000 INFO @ Mon, 03 Jun 2019 22:08:06: 16000000 INFO @ Mon, 03 Jun 2019 22:08:07: 17000000 INFO @ Mon, 03 Jun 2019 22:08:13: 19000000 INFO @ Mon, 03 Jun 2019 22:08:15: 17000000 INFO @ Mon, 03 Jun 2019 22:08:16: 18000000 INFO @ Mon, 03 Jun 2019 22:08:21: 20000000 INFO @ Mon, 03 Jun 2019 22:08:24: 18000000 INFO @ Mon, 03 Jun 2019 22:08:24: 19000000 INFO @ Mon, 03 Jun 2019 22:08:29: 21000000 INFO @ Mon, 03 Jun 2019 22:08:32: 20000000 INFO @ Mon, 03 Jun 2019 22:08:33: 19000000 INFO @ Mon, 03 Jun 2019 22:08:37: 22000000 INFO @ Mon, 03 Jun 2019 22:08:41: 21000000 INFO @ Mon, 03 Jun 2019 22:08:41: 20000000 INFO @ Mon, 03 Jun 2019 22:08:45: 23000000 INFO @ Mon, 03 Jun 2019 22:08:49: 22000000 INFO @ Mon, 03 Jun 2019 22:08:50: 21000000 INFO @ Mon, 03 Jun 2019 22:08:53: 24000000 INFO @ Mon, 03 Jun 2019 22:08:57: 23000000 INFO @ Mon, 03 Jun 2019 22:08:59: 22000000 INFO @ Mon, 03 Jun 2019 22:09:01: 25000000 INFO @ Mon, 03 Jun 2019 22:09:05: 24000000 INFO @ Mon, 03 Jun 2019 22:09:07: 23000000 INFO @ Mon, 03 Jun 2019 22:09:09: 26000000 INFO @ Mon, 03 Jun 2019 22:09:13: 25000000 INFO @ Mon, 03 Jun 2019 22:09:16: 24000000 INFO @ Mon, 03 Jun 2019 22:09:17: 27000000 INFO @ Mon, 03 Jun 2019 22:09:21: 26000000 INFO @ Mon, 03 Jun 2019 22:09:24: 25000000 INFO @ Mon, 03 Jun 2019 22:09:26: 28000000 INFO @ Mon, 03 Jun 2019 22:09:30: 27000000 INFO @ Mon, 03 Jun 2019 22:09:33: 26000000 INFO @ Mon, 03 Jun 2019 22:09:34: 29000000 INFO @ Mon, 03 Jun 2019 22:09:38: 28000000 INFO @ Mon, 03 Jun 2019 22:09:41: 27000000 INFO @ Mon, 03 Jun 2019 22:09:42: 30000000 INFO @ Mon, 03 Jun 2019 22:09:46: 29000000 INFO @ Mon, 03 Jun 2019 22:09:50: 31000000 INFO @ Mon, 03 Jun 2019 22:09:50: 28000000 INFO @ Mon, 03 Jun 2019 22:09:54: 30000000 INFO @ Mon, 03 Jun 2019 22:09:58: 32000000 INFO @ Mon, 03 Jun 2019 22:09:59: 29000000 INFO @ Mon, 03 Jun 2019 22:10:02: 31000000 INFO @ Mon, 03 Jun 2019 22:10:06: 33000000 INFO @ Mon, 03 Jun 2019 22:10:07: 30000000 INFO @ Mon, 03 Jun 2019 22:10:10: 32000000 INFO @ Mon, 03 Jun 2019 22:10:14: 34000000 INFO @ Mon, 03 Jun 2019 22:10:16: 31000000 INFO @ Mon, 03 Jun 2019 22:10:19: 33000000 INFO @ Mon, 03 Jun 2019 22:10:22: 35000000 INFO @ Mon, 03 Jun 2019 22:10:25: 32000000 INFO @ Mon, 03 Jun 2019 22:10:27: 34000000 INFO @ Mon, 03 Jun 2019 22:10:30: 36000000 INFO @ Mon, 03 Jun 2019 22:10:33: 33000000 INFO @ Mon, 03 Jun 2019 22:10:35: 35000000 INFO @ Mon, 03 Jun 2019 22:10:38: 37000000 INFO @ Mon, 03 Jun 2019 22:10:42: 34000000 INFO @ Mon, 03 Jun 2019 22:10:43: 36000000 INFO @ Mon, 03 Jun 2019 22:10:46: 38000000 INFO @ Mon, 03 Jun 2019 22:10:50: 35000000 INFO @ Mon, 03 Jun 2019 22:10:51: 37000000 INFO @ Mon, 03 Jun 2019 22:10:55: 39000000 INFO @ Mon, 03 Jun 2019 22:10:59: 36000000 INFO @ Mon, 03 Jun 2019 22:11:00: 38000000 INFO @ Mon, 03 Jun 2019 22:11:03: 40000000 INFO @ Mon, 03 Jun 2019 22:11:08: 37000000 INFO @ Mon, 03 Jun 2019 22:11:08: 39000000 INFO @ Mon, 03 Jun 2019 22:11:11: 41000000 INFO @ Mon, 03 Jun 2019 22:11:16: 40000000 INFO @ Mon, 03 Jun 2019 22:11:16: 38000000 INFO @ Mon, 03 Jun 2019 22:11:19: 42000000 INFO @ Mon, 03 Jun 2019 22:11:24: 41000000 INFO @ Mon, 03 Jun 2019 22:11:25: 39000000 INFO @ Mon, 03 Jun 2019 22:11:27: 43000000 INFO @ Mon, 03 Jun 2019 22:11:32: 42000000 INFO @ Mon, 03 Jun 2019 22:11:33: 40000000 INFO @ Mon, 03 Jun 2019 22:11:35: 44000000 INFO @ Mon, 03 Jun 2019 22:11:40: 43000000 INFO @ Mon, 03 Jun 2019 22:11:42: 41000000 INFO @ Mon, 03 Jun 2019 22:11:43: 45000000 INFO @ Mon, 03 Jun 2019 22:11:48: 44000000 INFO @ Mon, 03 Jun 2019 22:11:51: 42000000 INFO @ Mon, 03 Jun 2019 22:11:51: 46000000 INFO @ Mon, 03 Jun 2019 22:11:57: 45000000 INFO @ Mon, 03 Jun 2019 22:11:59: 43000000 INFO @ Mon, 03 Jun 2019 22:11:59: 47000000 INFO @ Mon, 03 Jun 2019 22:12:01: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:12:01: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:12:01: #1 total tags in treatment: 47141159 INFO @ Mon, 03 Jun 2019 22:12:01: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:12:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:12:02: #1 tags after filtering in treatment: 47141159 INFO @ Mon, 03 Jun 2019 22:12:02: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:12:02: #1 finished! INFO @ Mon, 03 Jun 2019 22:12:02: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:12:02: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:12:05: 46000000 INFO @ Mon, 03 Jun 2019 22:12:06: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 22:12:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:12:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 22:12:07: 44000000 INFO @ Mon, 03 Jun 2019 22:12:13: 47000000 INFO @ Mon, 03 Jun 2019 22:12:14: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:12:14: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:12:14: #1 total tags in treatment: 47141159 INFO @ Mon, 03 Jun 2019 22:12:14: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:12:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:12:15: #1 tags after filtering in treatment: 47141159 INFO @ Mon, 03 Jun 2019 22:12:15: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:12:15: #1 finished! INFO @ Mon, 03 Jun 2019 22:12:15: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:12:15: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:12:16: 45000000 INFO @ Mon, 03 Jun 2019 22:12:19: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 22:12:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:12:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 22:12:24: 46000000 INFO @ Mon, 03 Jun 2019 22:12:33: 47000000 INFO @ Mon, 03 Jun 2019 22:12:34: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 22:12:34: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 22:12:34: #1 total tags in treatment: 47141159 INFO @ Mon, 03 Jun 2019 22:12:34: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:12:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:12:35: #1 tags after filtering in treatment: 47141159 INFO @ Mon, 03 Jun 2019 22:12:35: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 22:12:35: #1 finished! INFO @ Mon, 03 Jun 2019 22:12:35: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:12:35: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:12:39: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 22:12:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 22:12:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495271/SRX495271.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。