Job ID = 1305084 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 23,779,885 reads read : 23,779,885 reads written : 23,779,885 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:28 23779885 reads; of these: 23779885 (100.00%) were unpaired; of these: 2749994 (11.56%) aligned 0 times 19054565 (80.13%) aligned exactly 1 time 1975326 (8.31%) aligned >1 times 88.44% overall alignment rate Time searching: 00:08:28 Overall time: 00:08:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4843300 / 21029891 = 0.2303 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:21:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:21:39: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:21:39: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:21:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:21:39: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:21:39: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:21:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:21:39: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:21:39: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:21:49: 1000000 INFO @ Mon, 03 Jun 2019 21:21:50: 1000000 INFO @ Mon, 03 Jun 2019 21:21:50: 1000000 INFO @ Mon, 03 Jun 2019 21:21:59: 2000000 INFO @ Mon, 03 Jun 2019 21:22:00: 2000000 INFO @ Mon, 03 Jun 2019 21:22:01: 2000000 INFO @ Mon, 03 Jun 2019 21:22:09: 3000000 INFO @ Mon, 03 Jun 2019 21:22:10: 3000000 INFO @ Mon, 03 Jun 2019 21:22:11: 3000000 INFO @ Mon, 03 Jun 2019 21:22:19: 4000000 INFO @ Mon, 03 Jun 2019 21:22:20: 4000000 INFO @ Mon, 03 Jun 2019 21:22:21: 4000000 INFO @ Mon, 03 Jun 2019 21:22:29: 5000000 INFO @ Mon, 03 Jun 2019 21:22:30: 5000000 INFO @ Mon, 03 Jun 2019 21:22:32: 5000000 INFO @ Mon, 03 Jun 2019 21:22:39: 6000000 INFO @ Mon, 03 Jun 2019 21:22:41: 6000000 INFO @ Mon, 03 Jun 2019 21:22:43: 6000000 INFO @ Mon, 03 Jun 2019 21:22:49: 7000000 INFO @ Mon, 03 Jun 2019 21:22:51: 7000000 INFO @ Mon, 03 Jun 2019 21:22:53: 7000000 INFO @ Mon, 03 Jun 2019 21:22:59: 8000000 INFO @ Mon, 03 Jun 2019 21:23:02: 8000000 INFO @ Mon, 03 Jun 2019 21:23:04: 8000000 INFO @ Mon, 03 Jun 2019 21:23:09: 9000000 INFO @ Mon, 03 Jun 2019 21:23:12: 9000000 INFO @ Mon, 03 Jun 2019 21:23:14: 9000000 INFO @ Mon, 03 Jun 2019 21:23:19: 10000000 INFO @ Mon, 03 Jun 2019 21:23:22: 10000000 INFO @ Mon, 03 Jun 2019 21:23:24: 10000000 INFO @ Mon, 03 Jun 2019 21:23:29: 11000000 INFO @ Mon, 03 Jun 2019 21:23:32: 11000000 INFO @ Mon, 03 Jun 2019 21:23:35: 11000000 INFO @ Mon, 03 Jun 2019 21:23:39: 12000000 INFO @ Mon, 03 Jun 2019 21:23:42: 12000000 INFO @ Mon, 03 Jun 2019 21:23:45: 12000000 INFO @ Mon, 03 Jun 2019 21:23:49: 13000000 INFO @ Mon, 03 Jun 2019 21:23:52: 13000000 INFO @ Mon, 03 Jun 2019 21:23:55: 13000000 INFO @ Mon, 03 Jun 2019 21:23:58: 14000000 INFO @ Mon, 03 Jun 2019 21:24:03: 14000000 INFO @ Mon, 03 Jun 2019 21:24:06: 14000000 INFO @ Mon, 03 Jun 2019 21:24:08: 15000000 INFO @ Mon, 03 Jun 2019 21:24:13: 15000000 INFO @ Mon, 03 Jun 2019 21:24:16: 15000000 INFO @ Mon, 03 Jun 2019 21:24:18: 16000000 INFO @ Mon, 03 Jun 2019 21:24:20: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:24:20: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:24:20: #1 total tags in treatment: 16186591 INFO @ Mon, 03 Jun 2019 21:24:20: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:24:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:24:20: #1 tags after filtering in treatment: 16186591 INFO @ Mon, 03 Jun 2019 21:24:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:24:20: #1 finished! INFO @ Mon, 03 Jun 2019 21:24:20: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:24:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:24:22: #2 number of paired peaks: 27 WARNING @ Mon, 03 Jun 2019 21:24:22: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:24:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:24:23: 16000000 INFO @ Mon, 03 Jun 2019 21:24:26: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:24:26: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:24:26: #1 total tags in treatment: 16186591 INFO @ Mon, 03 Jun 2019 21:24:26: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:24:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:24:26: 16000000 INFO @ Mon, 03 Jun 2019 21:24:26: #1 tags after filtering in treatment: 16186591 INFO @ Mon, 03 Jun 2019 21:24:26: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:24:26: #1 finished! INFO @ Mon, 03 Jun 2019 21:24:26: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:24:26: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:24:28: #2 number of paired peaks: 27 WARNING @ Mon, 03 Jun 2019 21:24:28: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:24:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:24:28: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:24:28: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:24:28: #1 total tags in treatment: 16186591 INFO @ Mon, 03 Jun 2019 21:24:28: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:24:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:24:28: #1 tags after filtering in treatment: 16186591 INFO @ Mon, 03 Jun 2019 21:24:28: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:24:28: #1 finished! INFO @ Mon, 03 Jun 2019 21:24:28: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:24:28: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:24:30: #2 number of paired peaks: 27 WARNING @ Mon, 03 Jun 2019 21:24:30: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:24:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495265/SRX495265.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。