Job ID = 1305040 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 24,355,341 reads read : 24,355,341 reads written : 24,355,341 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:33 24355341 reads; of these: 24355341 (100.00%) were unpaired; of these: 1502843 (6.17%) aligned 0 times 19913576 (81.76%) aligned exactly 1 time 2938922 (12.07%) aligned >1 times 93.83% overall alignment rate Time searching: 00:07:33 Overall time: 00:07:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4874980 / 22852498 = 0.2133 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:17:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:17:49: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:17:49: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:17:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:17:49: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:17:49: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:17:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:17:50: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:17:50: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:18:00: 1000000 INFO @ Mon, 03 Jun 2019 21:18:00: 1000000 INFO @ Mon, 03 Jun 2019 21:18:04: 1000000 INFO @ Mon, 03 Jun 2019 21:18:10: 2000000 INFO @ Mon, 03 Jun 2019 21:18:10: 2000000 INFO @ Mon, 03 Jun 2019 21:18:18: 2000000 INFO @ Mon, 03 Jun 2019 21:18:20: 3000000 INFO @ Mon, 03 Jun 2019 21:18:20: 3000000 INFO @ Mon, 03 Jun 2019 21:18:30: 4000000 INFO @ Mon, 03 Jun 2019 21:18:30: 4000000 INFO @ Mon, 03 Jun 2019 21:18:32: 3000000 INFO @ Mon, 03 Jun 2019 21:18:40: 5000000 INFO @ Mon, 03 Jun 2019 21:18:40: 5000000 INFO @ Mon, 03 Jun 2019 21:18:45: 4000000 INFO @ Mon, 03 Jun 2019 21:18:50: 6000000 INFO @ Mon, 03 Jun 2019 21:18:50: 6000000 INFO @ Mon, 03 Jun 2019 21:18:58: 5000000 INFO @ Mon, 03 Jun 2019 21:18:59: 7000000 INFO @ Mon, 03 Jun 2019 21:18:59: 7000000 INFO @ Mon, 03 Jun 2019 21:19:09: 8000000 INFO @ Mon, 03 Jun 2019 21:19:09: 8000000 INFO @ Mon, 03 Jun 2019 21:19:10: 6000000 INFO @ Mon, 03 Jun 2019 21:19:17: 9000000 INFO @ Mon, 03 Jun 2019 21:19:17: 9000000 INFO @ Mon, 03 Jun 2019 21:19:22: 7000000 INFO @ Mon, 03 Jun 2019 21:19:26: 10000000 INFO @ Mon, 03 Jun 2019 21:19:26: 10000000 INFO @ Mon, 03 Jun 2019 21:19:32: 8000000 INFO @ Mon, 03 Jun 2019 21:19:35: 11000000 INFO @ Mon, 03 Jun 2019 21:19:35: 11000000 INFO @ Mon, 03 Jun 2019 21:19:41: 9000000 INFO @ Mon, 03 Jun 2019 21:19:44: 12000000 INFO @ Mon, 03 Jun 2019 21:19:44: 12000000 INFO @ Mon, 03 Jun 2019 21:19:52: 10000000 INFO @ Mon, 03 Jun 2019 21:19:53: 13000000 INFO @ Mon, 03 Jun 2019 21:19:53: 13000000 INFO @ Mon, 03 Jun 2019 21:20:02: 11000000 INFO @ Mon, 03 Jun 2019 21:20:02: 14000000 INFO @ Mon, 03 Jun 2019 21:20:02: 14000000 INFO @ Mon, 03 Jun 2019 21:20:12: 15000000 INFO @ Mon, 03 Jun 2019 21:20:12: 15000000 INFO @ Mon, 03 Jun 2019 21:20:13: 12000000 INFO @ Mon, 03 Jun 2019 21:20:21: 16000000 INFO @ Mon, 03 Jun 2019 21:20:21: 16000000 INFO @ Mon, 03 Jun 2019 21:20:23: 13000000 INFO @ Mon, 03 Jun 2019 21:20:30: 17000000 INFO @ Mon, 03 Jun 2019 21:20:30: 17000000 INFO @ Mon, 03 Jun 2019 21:20:34: 14000000 INFO @ Mon, 03 Jun 2019 21:20:38: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:20:38: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:20:38: #1 total tags in treatment: 17977518 INFO @ Mon, 03 Jun 2019 21:20:38: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:20:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:20:38: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:20:38: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:20:38: #1 total tags in treatment: 17977518 INFO @ Mon, 03 Jun 2019 21:20:38: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:20:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:20:39: #1 tags after filtering in treatment: 17977518 INFO @ Mon, 03 Jun 2019 21:20:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:20:39: #1 finished! INFO @ Mon, 03 Jun 2019 21:20:39: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:20:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:20:39: #1 tags after filtering in treatment: 17977518 INFO @ Mon, 03 Jun 2019 21:20:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:20:39: #1 finished! INFO @ Mon, 03 Jun 2019 21:20:39: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:20:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:20:40: #2 number of paired peaks: 29 WARNING @ Mon, 03 Jun 2019 21:20:40: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:20:40: Process for pairing-model is terminated! INFO @ Mon, 03 Jun 2019 21:20:40: #2 number of paired peaks: 29 WARNING @ Mon, 03 Jun 2019 21:20:40: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:20:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.20_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:20:44: 15000000 INFO @ Mon, 03 Jun 2019 21:20:56: 16000000 INFO @ Mon, 03 Jun 2019 21:21:08: 17000000 INFO @ Mon, 03 Jun 2019 21:21:20: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:21:20: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:21:20: #1 total tags in treatment: 17977518 INFO @ Mon, 03 Jun 2019 21:21:20: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:21:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:21:20: #1 tags after filtering in treatment: 17977518 INFO @ Mon, 03 Jun 2019 21:21:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:21:20: #1 finished! INFO @ Mon, 03 Jun 2019 21:21:20: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:21:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:21:22: #2 number of paired peaks: 29 WARNING @ Mon, 03 Jun 2019 21:21:22: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:21:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495263/SRX495263.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。