Job ID = 1304871 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 20,889,019 reads read : 20,889,019 reads written : 20,889,019 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:59 20889019 reads; of these: 20889019 (100.00%) were unpaired; of these: 2896962 (13.87%) aligned 0 times 14305134 (68.48%) aligned exactly 1 time 3686923 (17.65%) aligned >1 times 86.13% overall alignment rate Time searching: 00:06:59 Overall time: 00:06:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3861086 / 17992057 = 0.2146 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:21:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:21:28: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:21:28: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:21:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:21:28: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:21:28: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:21:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:21:28: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:21:28: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:21:36: 1000000 INFO @ Mon, 03 Jun 2019 21:21:37: 1000000 INFO @ Mon, 03 Jun 2019 21:21:37: 1000000 INFO @ Mon, 03 Jun 2019 21:21:45: 2000000 INFO @ Mon, 03 Jun 2019 21:21:46: 2000000 INFO @ Mon, 03 Jun 2019 21:21:47: 2000000 INFO @ Mon, 03 Jun 2019 21:21:53: 3000000 INFO @ Mon, 03 Jun 2019 21:21:55: 3000000 INFO @ Mon, 03 Jun 2019 21:21:56: 3000000 INFO @ Mon, 03 Jun 2019 21:22:01: 4000000 INFO @ Mon, 03 Jun 2019 21:22:04: 4000000 INFO @ Mon, 03 Jun 2019 21:22:04: 4000000 INFO @ Mon, 03 Jun 2019 21:22:09: 5000000 INFO @ Mon, 03 Jun 2019 21:22:12: 5000000 INFO @ Mon, 03 Jun 2019 21:22:13: 5000000 INFO @ Mon, 03 Jun 2019 21:22:17: 6000000 INFO @ Mon, 03 Jun 2019 21:22:21: 6000000 INFO @ Mon, 03 Jun 2019 21:22:22: 6000000 INFO @ Mon, 03 Jun 2019 21:22:27: 7000000 INFO @ Mon, 03 Jun 2019 21:22:31: 7000000 INFO @ Mon, 03 Jun 2019 21:22:33: 7000000 INFO @ Mon, 03 Jun 2019 21:22:35: 8000000 INFO @ Mon, 03 Jun 2019 21:22:39: 8000000 INFO @ Mon, 03 Jun 2019 21:22:42: 8000000 INFO @ Mon, 03 Jun 2019 21:22:43: 9000000 INFO @ Mon, 03 Jun 2019 21:22:48: 9000000 INFO @ Mon, 03 Jun 2019 21:22:50: 9000000 INFO @ Mon, 03 Jun 2019 21:22:51: 10000000 INFO @ Mon, 03 Jun 2019 21:22:57: 10000000 INFO @ Mon, 03 Jun 2019 21:22:59: 10000000 INFO @ Mon, 03 Jun 2019 21:22:59: 11000000 INFO @ Mon, 03 Jun 2019 21:23:06: 11000000 INFO @ Mon, 03 Jun 2019 21:23:08: 12000000 INFO @ Mon, 03 Jun 2019 21:23:08: 11000000 INFO @ Mon, 03 Jun 2019 21:23:15: 12000000 INFO @ Mon, 03 Jun 2019 21:23:16: 13000000 INFO @ Mon, 03 Jun 2019 21:23:17: 12000000 INFO @ Mon, 03 Jun 2019 21:23:23: 13000000 INFO @ Mon, 03 Jun 2019 21:23:24: 14000000 INFO @ Mon, 03 Jun 2019 21:23:25: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:23:25: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:23:25: #1 total tags in treatment: 14130971 INFO @ Mon, 03 Jun 2019 21:23:25: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:23:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:23:25: #1 tags after filtering in treatment: 14130971 INFO @ Mon, 03 Jun 2019 21:23:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:23:25: #1 finished! INFO @ Mon, 03 Jun 2019 21:23:25: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:23:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:23:26: 13000000 INFO @ Mon, 03 Jun 2019 21:23:27: #2 number of paired peaks: 59 WARNING @ Mon, 03 Jun 2019 21:23:27: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:23:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:23:32: 14000000 INFO @ Mon, 03 Jun 2019 21:23:34: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:23:34: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:23:34: #1 total tags in treatment: 14130971 INFO @ Mon, 03 Jun 2019 21:23:34: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:23:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:23:34: #1 tags after filtering in treatment: 14130971 INFO @ Mon, 03 Jun 2019 21:23:34: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:23:34: #1 finished! INFO @ Mon, 03 Jun 2019 21:23:34: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:23:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:23:34: 14000000 INFO @ Mon, 03 Jun 2019 21:23:35: #2 number of paired peaks: 59 WARNING @ Mon, 03 Jun 2019 21:23:35: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:23:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 21:23:36: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 21:23:36: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 21:23:36: #1 total tags in treatment: 14130971 INFO @ Mon, 03 Jun 2019 21:23:36: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 21:23:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 21:23:36: #1 tags after filtering in treatment: 14130971 INFO @ Mon, 03 Jun 2019 21:23:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 21:23:36: #1 finished! INFO @ Mon, 03 Jun 2019 21:23:36: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 21:23:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 21:23:37: #2 number of paired peaks: 59 WARNING @ Mon, 03 Jun 2019 21:23:37: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 21:23:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495254/SRX495254.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。