Job ID = 6528267 SRX = SRX495253 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:33:21 prefetch.2.10.7: 1) Downloading 'SRR1198758'... 2020-06-29T14:33:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:33:57 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:33:57 prefetch.2.10.7: 'SRR1198758' is valid 2020-06-29T14:33:57 prefetch.2.10.7: 1) 'SRR1198758' was downloaded successfully Read 2188064 spots for SRR1198758/SRR1198758.sra Written 2188064 spots for SRR1198758/SRR1198758.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:32 2188064 reads; of these: 2188064 (100.00%) were unpaired; of these: 309526 (14.15%) aligned 0 times 1450037 (66.27%) aligned exactly 1 time 428501 (19.58%) aligned >1 times 85.85% overall alignment rate Time searching: 00:00:32 Overall time: 00:00:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 176583 / 1878538 = 0.0940 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:36:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:36:06: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:36:06: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:36:13: 1000000 INFO @ Mon, 29 Jun 2020 23:36:18: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:36:18: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:36:18: #1 total tags in treatment: 1701955 INFO @ Mon, 29 Jun 2020 23:36:18: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:36:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:36:18: #1 tags after filtering in treatment: 1701955 INFO @ Mon, 29 Jun 2020 23:36:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:36:18: #1 finished! INFO @ Mon, 29 Jun 2020 23:36:18: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:36:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:36:18: #2 number of paired peaks: 71 WARNING @ Mon, 29 Jun 2020 23:36:18: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:36:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:36:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:36:36: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:36:36: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:36:43: 1000000 INFO @ Mon, 29 Jun 2020 23:36:47: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:36:47: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:36:47: #1 total tags in treatment: 1701955 INFO @ Mon, 29 Jun 2020 23:36:47: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:36:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:36:47: #1 tags after filtering in treatment: 1701955 INFO @ Mon, 29 Jun 2020 23:36:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:36:47: #1 finished! INFO @ Mon, 29 Jun 2020 23:36:47: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:36:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:36:47: #2 number of paired peaks: 71 WARNING @ Mon, 29 Jun 2020 23:36:47: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:36:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:37:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:37:06: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:37:06: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:37:12: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:37:17: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:37:17: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:37:17: #1 total tags in treatment: 1701955 INFO @ Mon, 29 Jun 2020 23:37:17: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:37:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:37:17: #1 tags after filtering in treatment: 1701955 INFO @ Mon, 29 Jun 2020 23:37:17: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:37:17: #1 finished! INFO @ Mon, 29 Jun 2020 23:37:17: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:37:17: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:37:17: #2 number of paired peaks: 71 WARNING @ Mon, 29 Jun 2020 23:37:17: Too few paired peaks (71) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:37:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495253/SRX495253.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。