Job ID = 6498416 SRX = SRX495251 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:30:16 prefetch.2.10.7: 1) Downloading 'SRR1198756'... 2020-06-25T23:30:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:33:09 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:33:09 prefetch.2.10.7: 1) 'SRR1198756' was downloaded successfully Read 19875673 spots for SRR1198756/SRR1198756.sra Written 19875673 spots for SRR1198756/SRR1198756.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:43 19875673 reads; of these: 19875673 (100.00%) were unpaired; of these: 806362 (4.06%) aligned 0 times 15987090 (80.44%) aligned exactly 1 time 3082221 (15.51%) aligned >1 times 95.94% overall alignment rate Time searching: 00:05:43 Overall time: 00:05:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3389491 / 19069311 = 0.1777 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:44:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:44:42: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:44:42: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:44:48: 1000000 INFO @ Fri, 26 Jun 2020 08:44:53: 2000000 INFO @ Fri, 26 Jun 2020 08:44:59: 3000000 INFO @ Fri, 26 Jun 2020 08:45:05: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:45:10: 5000000 INFO @ Fri, 26 Jun 2020 08:45:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:45:12: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:45:12: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:45:16: 6000000 INFO @ Fri, 26 Jun 2020 08:45:17: 1000000 INFO @ Fri, 26 Jun 2020 08:45:22: 7000000 INFO @ Fri, 26 Jun 2020 08:45:23: 2000000 INFO @ Fri, 26 Jun 2020 08:45:28: 3000000 INFO @ Fri, 26 Jun 2020 08:45:28: 8000000 INFO @ Fri, 26 Jun 2020 08:45:33: 4000000 INFO @ Fri, 26 Jun 2020 08:45:34: 9000000 INFO @ Fri, 26 Jun 2020 08:45:39: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:45:40: 10000000 INFO @ Fri, 26 Jun 2020 08:45:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:45:42: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:45:42: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:45:44: 6000000 INFO @ Fri, 26 Jun 2020 08:45:47: 11000000 INFO @ Fri, 26 Jun 2020 08:45:48: 1000000 INFO @ Fri, 26 Jun 2020 08:45:50: 7000000 INFO @ Fri, 26 Jun 2020 08:45:53: 12000000 INFO @ Fri, 26 Jun 2020 08:45:53: 2000000 INFO @ Fri, 26 Jun 2020 08:45:55: 8000000 INFO @ Fri, 26 Jun 2020 08:45:59: 13000000 INFO @ Fri, 26 Jun 2020 08:45:59: 3000000 INFO @ Fri, 26 Jun 2020 08:46:01: 9000000 INFO @ Fri, 26 Jun 2020 08:46:05: 4000000 INFO @ Fri, 26 Jun 2020 08:46:05: 14000000 INFO @ Fri, 26 Jun 2020 08:46:07: 10000000 INFO @ Fri, 26 Jun 2020 08:46:10: 5000000 INFO @ Fri, 26 Jun 2020 08:46:11: 15000000 INFO @ Fri, 26 Jun 2020 08:46:12: 11000000 INFO @ Fri, 26 Jun 2020 08:46:15: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:46:15: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:46:15: #1 total tags in treatment: 15679820 INFO @ Fri, 26 Jun 2020 08:46:15: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:46:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:46:16: #1 tags after filtering in treatment: 15679820 INFO @ Fri, 26 Jun 2020 08:46:16: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:46:16: #1 finished! INFO @ Fri, 26 Jun 2020 08:46:16: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:46:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:46:16: 6000000 INFO @ Fri, 26 Jun 2020 08:46:17: #2 number of paired peaks: 48 WARNING @ Fri, 26 Jun 2020 08:46:17: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:46:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:46:18: 12000000 INFO @ Fri, 26 Jun 2020 08:46:21: 7000000 INFO @ Fri, 26 Jun 2020 08:46:23: 13000000 INFO @ Fri, 26 Jun 2020 08:46:27: 8000000 INFO @ Fri, 26 Jun 2020 08:46:29: 14000000 INFO @ Fri, 26 Jun 2020 08:46:32: 9000000 INFO @ Fri, 26 Jun 2020 08:46:34: 15000000 INFO @ Fri, 26 Jun 2020 08:46:38: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:46:38: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:46:38: #1 total tags in treatment: 15679820 INFO @ Fri, 26 Jun 2020 08:46:38: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:46:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:46:38: 10000000 INFO @ Fri, 26 Jun 2020 08:46:38: #1 tags after filtering in treatment: 15679820 INFO @ Fri, 26 Jun 2020 08:46:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:46:38: #1 finished! INFO @ Fri, 26 Jun 2020 08:46:38: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:46:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:46:39: #2 number of paired peaks: 48 WARNING @ Fri, 26 Jun 2020 08:46:39: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:46:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:46:43: 11000000 INFO @ Fri, 26 Jun 2020 08:46:48: 12000000 INFO @ Fri, 26 Jun 2020 08:46:53: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:46:58: 14000000 INFO @ Fri, 26 Jun 2020 08:47:04: 15000000 INFO @ Fri, 26 Jun 2020 08:47:07: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:47:07: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:47:07: #1 total tags in treatment: 15679820 INFO @ Fri, 26 Jun 2020 08:47:07: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:47:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:47:07: #1 tags after filtering in treatment: 15679820 INFO @ Fri, 26 Jun 2020 08:47:07: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:47:07: #1 finished! INFO @ Fri, 26 Jun 2020 08:47:07: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:47:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:47:08: #2 number of paired peaks: 48 WARNING @ Fri, 26 Jun 2020 08:47:08: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:47:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495251/SRX495251.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。