Job ID = 6498410 SRX = SRX495245 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:16:41 prefetch.2.10.7: 1) Downloading 'SRR1198750'... 2020-06-25T23:16:41 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:21:48 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:21:48 prefetch.2.10.7: 1) 'SRR1198750' was downloaded successfully Read 30861048 spots for SRR1198750/SRR1198750.sra Written 30861048 spots for SRR1198750/SRR1198750.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:27 30861048 reads; of these: 30861048 (100.00%) were unpaired; of these: 3582052 (11.61%) aligned 0 times 22320445 (72.33%) aligned exactly 1 time 4958551 (16.07%) aligned >1 times 88.39% overall alignment rate Time searching: 00:08:27 Overall time: 00:08:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5593325 / 27278996 = 0.2050 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:38:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:38:38: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:38:38: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:38:44: 1000000 INFO @ Fri, 26 Jun 2020 08:38:50: 2000000 INFO @ Fri, 26 Jun 2020 08:38:55: 3000000 INFO @ Fri, 26 Jun 2020 08:39:01: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:39:07: 5000000 INFO @ Fri, 26 Jun 2020 08:39:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:39:09: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:39:09: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:39:14: 6000000 INFO @ Fri, 26 Jun 2020 08:39:16: 1000000 INFO @ Fri, 26 Jun 2020 08:39:20: 7000000 INFO @ Fri, 26 Jun 2020 08:39:22: 2000000 INFO @ Fri, 26 Jun 2020 08:39:27: 8000000 INFO @ Fri, 26 Jun 2020 08:39:29: 3000000 INFO @ Fri, 26 Jun 2020 08:39:33: 9000000 INFO @ Fri, 26 Jun 2020 08:39:35: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:39:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:39:38: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:39:38: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:39:40: 10000000 INFO @ Fri, 26 Jun 2020 08:39:42: 5000000 INFO @ Fri, 26 Jun 2020 08:39:44: 1000000 INFO @ Fri, 26 Jun 2020 08:39:47: 11000000 INFO @ Fri, 26 Jun 2020 08:39:48: 6000000 INFO @ Fri, 26 Jun 2020 08:39:51: 2000000 INFO @ Fri, 26 Jun 2020 08:39:53: 12000000 INFO @ Fri, 26 Jun 2020 08:39:55: 7000000 INFO @ Fri, 26 Jun 2020 08:39:58: 3000000 INFO @ Fri, 26 Jun 2020 08:40:00: 13000000 INFO @ Fri, 26 Jun 2020 08:40:01: 8000000 INFO @ Fri, 26 Jun 2020 08:40:04: 4000000 INFO @ Fri, 26 Jun 2020 08:40:06: 14000000 INFO @ Fri, 26 Jun 2020 08:40:08: 9000000 INFO @ Fri, 26 Jun 2020 08:40:11: 5000000 INFO @ Fri, 26 Jun 2020 08:40:13: 15000000 INFO @ Fri, 26 Jun 2020 08:40:14: 10000000 INFO @ Fri, 26 Jun 2020 08:40:18: 6000000 INFO @ Fri, 26 Jun 2020 08:40:19: 16000000 INFO @ Fri, 26 Jun 2020 08:40:21: 11000000 INFO @ Fri, 26 Jun 2020 08:40:24: 7000000 INFO @ Fri, 26 Jun 2020 08:40:26: 17000000 INFO @ Fri, 26 Jun 2020 08:40:27: 12000000 INFO @ Fri, 26 Jun 2020 08:40:31: 8000000 INFO @ Fri, 26 Jun 2020 08:40:33: 18000000 INFO @ Fri, 26 Jun 2020 08:40:34: 13000000 INFO @ Fri, 26 Jun 2020 08:40:37: 9000000 INFO @ Fri, 26 Jun 2020 08:40:39: 19000000 INFO @ Fri, 26 Jun 2020 08:40:40: 14000000 INFO @ Fri, 26 Jun 2020 08:40:44: 10000000 INFO @ Fri, 26 Jun 2020 08:40:46: 20000000 INFO @ Fri, 26 Jun 2020 08:40:47: 15000000 INFO @ Fri, 26 Jun 2020 08:40:50: 11000000 INFO @ Fri, 26 Jun 2020 08:40:52: 21000000 INFO @ Fri, 26 Jun 2020 08:40:53: 16000000 INFO @ Fri, 26 Jun 2020 08:40:57: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:40:57: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:40:57: #1 total tags in treatment: 21685671 INFO @ Fri, 26 Jun 2020 08:40:57: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:40:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:40:57: 12000000 INFO @ Fri, 26 Jun 2020 08:40:57: #1 tags after filtering in treatment: 21685671 INFO @ Fri, 26 Jun 2020 08:40:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:40:57: #1 finished! INFO @ Fri, 26 Jun 2020 08:40:57: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:40:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:40:59: #2 number of paired peaks: 10 WARNING @ Fri, 26 Jun 2020 08:40:59: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:40:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:41:00: 17000000 INFO @ Fri, 26 Jun 2020 08:41:03: 13000000 INFO @ Fri, 26 Jun 2020 08:41:06: 18000000 INFO @ Fri, 26 Jun 2020 08:41:10: 14000000 INFO @ Fri, 26 Jun 2020 08:41:13: 19000000 INFO @ Fri, 26 Jun 2020 08:41:16: 15000000 INFO @ Fri, 26 Jun 2020 08:41:20: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 08:41:23: 16000000 INFO @ Fri, 26 Jun 2020 08:41:26: 21000000 INFO @ Fri, 26 Jun 2020 08:41:30: 17000000 INFO @ Fri, 26 Jun 2020 08:41:31: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:41:31: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:41:31: #1 total tags in treatment: 21685671 INFO @ Fri, 26 Jun 2020 08:41:31: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:41:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:41:31: #1 tags after filtering in treatment: 21685671 INFO @ Fri, 26 Jun 2020 08:41:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:41:31: #1 finished! INFO @ Fri, 26 Jun 2020 08:41:31: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:41:31: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:41:33: #2 number of paired peaks: 10 WARNING @ Fri, 26 Jun 2020 08:41:33: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:41:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 08:41:36: 18000000 INFO @ Fri, 26 Jun 2020 08:41:42: 19000000 INFO @ Fri, 26 Jun 2020 08:41:48: 20000000 INFO @ Fri, 26 Jun 2020 08:41:54: 21000000 INFO @ Fri, 26 Jun 2020 08:41:58: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 08:41:58: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 08:41:58: #1 total tags in treatment: 21685671 INFO @ Fri, 26 Jun 2020 08:41:58: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 08:41:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 08:41:59: #1 tags after filtering in treatment: 21685671 INFO @ Fri, 26 Jun 2020 08:41:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 08:41:59: #1 finished! INFO @ Fri, 26 Jun 2020 08:41:59: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 08:41:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 08:42:00: #2 number of paired peaks: 10 WARNING @ Fri, 26 Jun 2020 08:42:00: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 08:42:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495245/SRX495245.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。