Job ID = 6498409 SRX = SRX495244 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:43:37 prefetch.2.10.7: 1) Downloading 'SRR1198749'... 2020-06-25T23:43:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:46:56 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:46:56 prefetch.2.10.7: 1) 'SRR1198749' was downloaded successfully Read 28441419 spots for SRR1198749/SRR1198749.sra Written 28441419 spots for SRR1198749/SRR1198749.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:25 28441419 reads; of these: 28441419 (100.00%) were unpaired; of these: 6495160 (22.84%) aligned 0 times 18078926 (63.57%) aligned exactly 1 time 3867333 (13.60%) aligned >1 times 77.16% overall alignment rate Time searching: 00:06:25 Overall time: 00:06:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4410453 / 21946259 = 0.2010 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 08:59:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 08:59:52: #1 read tag files... INFO @ Fri, 26 Jun 2020 08:59:52: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 08:59:56: 1000000 INFO @ Fri, 26 Jun 2020 09:00:01: 2000000 INFO @ Fri, 26 Jun 2020 09:00:06: 3000000 INFO @ Fri, 26 Jun 2020 09:00:11: 4000000 INFO @ Fri, 26 Jun 2020 09:00:15: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:00:20: 6000000 INFO @ Fri, 26 Jun 2020 09:00:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:00:21: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:00:21: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:00:25: 7000000 INFO @ Fri, 26 Jun 2020 09:00:26: 1000000 INFO @ Fri, 26 Jun 2020 09:00:30: 8000000 INFO @ Fri, 26 Jun 2020 09:00:31: 2000000 INFO @ Fri, 26 Jun 2020 09:00:35: 9000000 INFO @ Fri, 26 Jun 2020 09:00:36: 3000000 INFO @ Fri, 26 Jun 2020 09:00:40: 10000000 INFO @ Fri, 26 Jun 2020 09:00:41: 4000000 INFO @ Fri, 26 Jun 2020 09:00:45: 11000000 INFO @ Fri, 26 Jun 2020 09:00:46: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:00:50: 12000000 INFO @ Fri, 26 Jun 2020 09:00:51: 6000000 INFO @ Fri, 26 Jun 2020 09:00:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:00:52: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:00:52: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:00:55: 13000000 INFO @ Fri, 26 Jun 2020 09:00:56: 7000000 INFO @ Fri, 26 Jun 2020 09:00:57: 1000000 INFO @ Fri, 26 Jun 2020 09:01:00: 14000000 INFO @ Fri, 26 Jun 2020 09:01:01: 8000000 INFO @ Fri, 26 Jun 2020 09:01:02: 2000000 INFO @ Fri, 26 Jun 2020 09:01:05: 15000000 INFO @ Fri, 26 Jun 2020 09:01:07: 9000000 INFO @ Fri, 26 Jun 2020 09:01:07: 3000000 INFO @ Fri, 26 Jun 2020 09:01:11: 16000000 INFO @ Fri, 26 Jun 2020 09:01:12: 10000000 INFO @ Fri, 26 Jun 2020 09:01:12: 4000000 INFO @ Fri, 26 Jun 2020 09:01:16: 17000000 INFO @ Fri, 26 Jun 2020 09:01:17: 11000000 INFO @ Fri, 26 Jun 2020 09:01:17: 5000000 INFO @ Fri, 26 Jun 2020 09:01:19: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:01:19: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:01:19: #1 total tags in treatment: 17535806 INFO @ Fri, 26 Jun 2020 09:01:19: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:01:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:01:19: #1 tags after filtering in treatment: 17535806 INFO @ Fri, 26 Jun 2020 09:01:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:01:19: #1 finished! INFO @ Fri, 26 Jun 2020 09:01:19: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:01:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:01:20: #2 number of paired peaks: 4 WARNING @ Fri, 26 Jun 2020 09:01:20: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:01:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:01:22: 12000000 INFO @ Fri, 26 Jun 2020 09:01:22: 6000000 INFO @ Fri, 26 Jun 2020 09:01:27: 13000000 INFO @ Fri, 26 Jun 2020 09:01:27: 7000000 INFO @ Fri, 26 Jun 2020 09:01:32: 14000000 INFO @ Fri, 26 Jun 2020 09:01:32: 8000000 INFO @ Fri, 26 Jun 2020 09:01:37: 15000000 INFO @ Fri, 26 Jun 2020 09:01:37: 9000000 INFO @ Fri, 26 Jun 2020 09:01:42: 16000000 INFO @ Fri, 26 Jun 2020 09:01:42: 10000000 INFO @ Fri, 26 Jun 2020 09:01:47: 17000000 INFO @ Fri, 26 Jun 2020 09:01:47: 11000000 INFO @ Fri, 26 Jun 2020 09:01:50: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:01:50: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:01:50: #1 total tags in treatment: 17535806 INFO @ Fri, 26 Jun 2020 09:01:50: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:01:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:01:50: #1 tags after filtering in treatment: 17535806 INFO @ Fri, 26 Jun 2020 09:01:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:01:50: #1 finished! INFO @ Fri, 26 Jun 2020 09:01:50: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:01:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:01:51: #2 number of paired peaks: 4 WARNING @ Fri, 26 Jun 2020 09:01:51: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:01:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:01:52: 12000000 INFO @ Fri, 26 Jun 2020 09:01:57: 13000000 INFO @ Fri, 26 Jun 2020 09:02:02: 14000000 INFO @ Fri, 26 Jun 2020 09:02:07: 15000000 INFO @ Fri, 26 Jun 2020 09:02:12: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:02:17: 17000000 INFO @ Fri, 26 Jun 2020 09:02:19: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:02:19: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:02:19: #1 total tags in treatment: 17535806 INFO @ Fri, 26 Jun 2020 09:02:19: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:02:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:02:20: #1 tags after filtering in treatment: 17535806 INFO @ Fri, 26 Jun 2020 09:02:20: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:02:20: #1 finished! INFO @ Fri, 26 Jun 2020 09:02:20: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:02:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:02:21: #2 number of paired peaks: 4 WARNING @ Fri, 26 Jun 2020 09:02:21: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:02:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495244/SRX495244.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。