Job ID = 6498407 SRX = SRX495242 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T00:06:35 prefetch.2.10.7: 1) Downloading 'SRR1198747'... 2020-06-26T00:06:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:08:45 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:08:45 prefetch.2.10.7: 1) 'SRR1198747' was downloaded successfully Read 22749432 spots for SRR1198747/SRR1198747.sra Written 22749432 spots for SRR1198747/SRR1198747.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:02 22749432 reads; of these: 22749432 (100.00%) were unpaired; of these: 978782 (4.30%) aligned 0 times 17901616 (78.69%) aligned exactly 1 time 3869034 (17.01%) aligned >1 times 95.70% overall alignment rate Time searching: 00:07:02 Overall time: 00:07:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3776966 / 21770650 = 0.1735 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:23:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:23:18: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:23:18: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:23:24: 1000000 INFO @ Fri, 26 Jun 2020 09:23:30: 2000000 INFO @ Fri, 26 Jun 2020 09:23:37: 3000000 INFO @ Fri, 26 Jun 2020 09:23:43: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:23:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:23:48: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:23:48: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:23:49: 5000000 INFO @ Fri, 26 Jun 2020 09:23:55: 1000000 INFO @ Fri, 26 Jun 2020 09:23:56: 6000000 INFO @ Fri, 26 Jun 2020 09:24:03: 7000000 INFO @ Fri, 26 Jun 2020 09:24:03: 2000000 INFO @ Fri, 26 Jun 2020 09:24:10: 8000000 INFO @ Fri, 26 Jun 2020 09:24:11: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:24:17: 9000000 INFO @ Fri, 26 Jun 2020 09:24:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:24:18: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:24:18: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:24:19: 4000000 INFO @ Fri, 26 Jun 2020 09:24:25: 10000000 INFO @ Fri, 26 Jun 2020 09:24:25: 1000000 INFO @ Fri, 26 Jun 2020 09:24:27: 5000000 INFO @ Fri, 26 Jun 2020 09:24:32: 11000000 INFO @ Fri, 26 Jun 2020 09:24:33: 2000000 INFO @ Fri, 26 Jun 2020 09:24:35: 6000000 INFO @ Fri, 26 Jun 2020 09:24:39: 12000000 INFO @ Fri, 26 Jun 2020 09:24:40: 3000000 INFO @ Fri, 26 Jun 2020 09:24:43: 7000000 INFO @ Fri, 26 Jun 2020 09:24:46: 13000000 INFO @ Fri, 26 Jun 2020 09:24:48: 4000000 INFO @ Fri, 26 Jun 2020 09:24:51: 8000000 INFO @ Fri, 26 Jun 2020 09:24:53: 14000000 INFO @ Fri, 26 Jun 2020 09:24:55: 5000000 INFO @ Fri, 26 Jun 2020 09:24:59: 9000000 INFO @ Fri, 26 Jun 2020 09:25:01: 15000000 INFO @ Fri, 26 Jun 2020 09:25:02: 6000000 INFO @ Fri, 26 Jun 2020 09:25:07: 10000000 INFO @ Fri, 26 Jun 2020 09:25:08: 16000000 INFO @ Fri, 26 Jun 2020 09:25:10: 7000000 INFO @ Fri, 26 Jun 2020 09:25:15: 11000000 INFO @ Fri, 26 Jun 2020 09:25:15: 17000000 INFO @ Fri, 26 Jun 2020 09:25:17: 8000000 INFO @ Fri, 26 Jun 2020 09:25:23: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:25:23: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:25:23: #1 total tags in treatment: 17993684 INFO @ Fri, 26 Jun 2020 09:25:23: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:25:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:25:23: #1 tags after filtering in treatment: 17993684 INFO @ Fri, 26 Jun 2020 09:25:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:25:23: #1 finished! INFO @ Fri, 26 Jun 2020 09:25:23: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:25:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:25:23: 12000000 INFO @ Fri, 26 Jun 2020 09:25:24: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 09:25:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:25:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.05_peaks.narrowPeak: No such file or directory INFO @ Fri, 26 Jun 2020 09:25:25: 9000000 pass1 - making usageList (0 chroms): 48 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:25:31: 13000000 INFO @ Fri, 26 Jun 2020 09:25:32: 10000000 INFO @ Fri, 26 Jun 2020 09:25:39: 14000000 INFO @ Fri, 26 Jun 2020 09:25:39: 11000000 INFO @ Fri, 26 Jun 2020 09:25:46: 15000000 INFO @ Fri, 26 Jun 2020 09:25:47: 12000000 INFO @ Fri, 26 Jun 2020 09:25:54: 16000000 INFO @ Fri, 26 Jun 2020 09:25:54: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:26:01: 17000000 INFO @ Fri, 26 Jun 2020 09:26:01: 14000000 INFO @ Fri, 26 Jun 2020 09:26:09: 15000000 INFO @ Fri, 26 Jun 2020 09:26:09: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:26:09: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:26:09: #1 total tags in treatment: 17993684 INFO @ Fri, 26 Jun 2020 09:26:09: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:26:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:26:10: #1 tags after filtering in treatment: 17993684 INFO @ Fri, 26 Jun 2020 09:26:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:26:10: #1 finished! INFO @ Fri, 26 Jun 2020 09:26:10: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:26:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:26:11: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 09:26:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:26:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:26:15: 16000000 INFO @ Fri, 26 Jun 2020 09:26:22: 17000000 INFO @ Fri, 26 Jun 2020 09:26:29: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:26:29: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:26:29: #1 total tags in treatment: 17993684 INFO @ Fri, 26 Jun 2020 09:26:29: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:26:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:26:29: #1 tags after filtering in treatment: 17993684 INFO @ Fri, 26 Jun 2020 09:26:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:26:29: #1 finished! INFO @ Fri, 26 Jun 2020 09:26:29: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:26:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:26:30: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 09:26:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:26:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 5 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495242/SRX495242.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。