Job ID = 6498406 SRX = SRX495241 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T00:16:51 prefetch.2.10.7: 1) Downloading 'SRR1198746'... 2020-06-26T00:16:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T00:18:49 prefetch.2.10.7: HTTPS download succeed 2020-06-26T00:18:49 prefetch.2.10.7: 1) 'SRR1198746' was downloaded successfully Read 22749432 spots for SRR1198746/SRR1198746.sra Written 22749432 spots for SRR1198746/SRR1198746.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:53 22749432 reads; of these: 22749432 (100.00%) were unpaired; of these: 978727 (4.30%) aligned 0 times 17901565 (78.69%) aligned exactly 1 time 3869140 (17.01%) aligned >1 times 95.70% overall alignment rate Time searching: 00:05:53 Overall time: 00:05:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3776842 / 21770705 = 0.1735 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:32:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:32:16: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:32:16: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:32:22: 1000000 INFO @ Fri, 26 Jun 2020 09:32:27: 2000000 INFO @ Fri, 26 Jun 2020 09:32:33: 3000000 INFO @ Fri, 26 Jun 2020 09:32:38: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:32:43: 5000000 INFO @ Fri, 26 Jun 2020 09:32:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:32:45: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:32:45: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:32:49: 6000000 INFO @ Fri, 26 Jun 2020 09:32:51: 1000000 INFO @ Fri, 26 Jun 2020 09:32:55: 7000000 INFO @ Fri, 26 Jun 2020 09:32:57: 2000000 INFO @ Fri, 26 Jun 2020 09:33:01: 8000000 INFO @ Fri, 26 Jun 2020 09:33:03: 3000000 INFO @ Fri, 26 Jun 2020 09:33:07: 9000000 INFO @ Fri, 26 Jun 2020 09:33:09: 4000000 INFO @ Fri, 26 Jun 2020 09:33:13: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:33:15: 5000000 INFO @ Fri, 26 Jun 2020 09:33:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:33:15: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:33:15: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:33:19: 11000000 INFO @ Fri, 26 Jun 2020 09:33:21: 1000000 INFO @ Fri, 26 Jun 2020 09:33:21: 6000000 INFO @ Fri, 26 Jun 2020 09:33:25: 12000000 INFO @ Fri, 26 Jun 2020 09:33:26: 2000000 INFO @ Fri, 26 Jun 2020 09:33:27: 7000000 INFO @ Fri, 26 Jun 2020 09:33:31: 13000000 INFO @ Fri, 26 Jun 2020 09:33:32: 3000000 INFO @ Fri, 26 Jun 2020 09:33:33: 8000000 INFO @ Fri, 26 Jun 2020 09:33:37: 4000000 INFO @ Fri, 26 Jun 2020 09:33:37: 14000000 INFO @ Fri, 26 Jun 2020 09:33:40: 9000000 INFO @ Fri, 26 Jun 2020 09:33:42: 5000000 INFO @ Fri, 26 Jun 2020 09:33:44: 15000000 INFO @ Fri, 26 Jun 2020 09:33:46: 10000000 INFO @ Fri, 26 Jun 2020 09:33:48: 6000000 INFO @ Fri, 26 Jun 2020 09:33:50: 16000000 INFO @ Fri, 26 Jun 2020 09:33:52: 11000000 INFO @ Fri, 26 Jun 2020 09:33:53: 7000000 INFO @ Fri, 26 Jun 2020 09:33:56: 17000000 INFO @ Fri, 26 Jun 2020 09:33:58: 12000000 INFO @ Fri, 26 Jun 2020 09:33:58: 8000000 INFO @ Fri, 26 Jun 2020 09:34:03: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:34:03: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:34:03: #1 total tags in treatment: 17993863 INFO @ Fri, 26 Jun 2020 09:34:03: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:34:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:34:03: #1 tags after filtering in treatment: 17993863 INFO @ Fri, 26 Jun 2020 09:34:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:34:03: #1 finished! INFO @ Fri, 26 Jun 2020 09:34:03: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:34:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:34:04: 9000000 INFO @ Fri, 26 Jun 2020 09:34:04: 13000000 INFO @ Fri, 26 Jun 2020 09:34:04: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 09:34:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:34:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:34:09: 10000000 INFO @ Fri, 26 Jun 2020 09:34:10: 14000000 INFO @ Fri, 26 Jun 2020 09:34:15: 11000000 INFO @ Fri, 26 Jun 2020 09:34:16: 15000000 INFO @ Fri, 26 Jun 2020 09:34:20: 12000000 INFO @ Fri, 26 Jun 2020 09:34:22: 16000000 INFO @ Fri, 26 Jun 2020 09:34:25: 13000000 INFO @ Fri, 26 Jun 2020 09:34:27: 17000000 INFO @ Fri, 26 Jun 2020 09:34:31: 14000000 INFO @ Fri, 26 Jun 2020 09:34:33: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:34:33: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:34:33: #1 total tags in treatment: 17993863 INFO @ Fri, 26 Jun 2020 09:34:33: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:34:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:34:33: #1 tags after filtering in treatment: 17993863 INFO @ Fri, 26 Jun 2020 09:34:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:34:33: #1 finished! INFO @ Fri, 26 Jun 2020 09:34:33: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:34:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:34:34: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 09:34:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:34:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:34:37: 15000000 INFO @ Fri, 26 Jun 2020 09:34:42: 16000000 INFO @ Fri, 26 Jun 2020 09:34:48: 17000000 INFO @ Fri, 26 Jun 2020 09:34:54: #1 tag size is determined as 44 bps INFO @ Fri, 26 Jun 2020 09:34:54: #1 tag size = 44 INFO @ Fri, 26 Jun 2020 09:34:54: #1 total tags in treatment: 17993863 INFO @ Fri, 26 Jun 2020 09:34:54: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:34:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:34:54: #1 tags after filtering in treatment: 17993863 INFO @ Fri, 26 Jun 2020 09:34:54: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:34:54: #1 finished! INFO @ Fri, 26 Jun 2020 09:34:54: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:34:54: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:34:55: #2 number of paired peaks: 0 WARNING @ Fri, 26 Jun 2020 09:34:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:34:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX495241/SRX495241.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。